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Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Laennecia confusa.

Martínez Ruiz MG, Richard-Greenblatt M, Juárez ZN, Av-Gay Y, Bach H, Hernández LR - ScientificWorldJournal (2012)

Bottom Line: The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G.All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages.In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula, PUE, Mexico. luisr.hernandez@udlap.mx

ABSTRACT
The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G. L. Nesom (Asteraceae). Fractions from the hexane, chloroform, methanol, and aqueous extracts were analyzed for antibacterial, antifungal, anti-inflammatory, and antiparasitic activities. The antimicrobial activity of the extracts and fractions was assessed on bacterial and fungal strains, in addition to the protozoa Leishmania donovani, using a microdilution assay. The propensity of the plant's compounds to produce adverse effects on human health was also evaluated using propidium iodine to identify damage to human macrophages. The anti-inflammatory activity of the extracts and fractions was investigated by measuring the secretion of interleukin-6. Chemical analyses demonstrated the presence of flavonoids, cyanogenic and cardiotonic glycosides, saponins, sesquiterpene lactones, and triterpenes in the chloroform extract. A number of extracts and fractions show antibacterial activity. Of particular interest is antibacterial activity against Staphylococcus aureus and its relative methicillin-resistant strain, MRSA. Hexanic and chloroformic fractions also exhibit antifungal activity and two extracts and the fraction CE 2 antiparasitic activity against Leishmania donovani. All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages. In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

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Related in: MedlinePlus

Anti-inflammatory activity of extracts and fractions from Laennecia confusa. An inflammatory process was elicited by exposing macrophages to LPS. Crude extracts were exposed to macrophages before the addition of LPS. The anti-inflammatory process was evaluated by measuring the secretion of IL-6 into the supernatant. Shown is the mean ± SD of three independent experiments. Numbers on the bars indicate the concentration of IL-6 in pg/mL. HEE, CEE, MEE, and AEE are hexane, chloroform, methanol, and aqueous extracts, respectively. *P value < 0.0001.
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fig4: Anti-inflammatory activity of extracts and fractions from Laennecia confusa. An inflammatory process was elicited by exposing macrophages to LPS. Crude extracts were exposed to macrophages before the addition of LPS. The anti-inflammatory process was evaluated by measuring the secretion of IL-6 into the supernatant. Shown is the mean ± SD of three independent experiments. Numbers on the bars indicate the concentration of IL-6 in pg/mL. HEE, CEE, MEE, and AEE are hexane, chloroform, methanol, and aqueous extracts, respectively. *P value < 0.0001.

Mentions: Human-derived macrophages were exposed to each of the extracts and then an inflammatory response was induced with LPS. To evaluate the inflammatory response, the secretion of the pro- and anti-inflammatory IL-6 cytokine was measured in the supernatant of the cultures. A concentration of 580 pg/mL of IL-6 was measured in the supernatant when LPS was added to the macrophages, while levels of 233 and 251 pg/mL were measured when these cells were pretreated with HEE and MEE, respectively (Figure 4). These values represent a reduction of approximately 60% in the secretion of IL-6. Similar results were measured when macrophages were exposed to prednisolone or dexamethasone, suggesting both extracts contains anti-inflammatory compounds. No effects were observed when either the CEE or AEE was supplemented to the cultures.


Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Laennecia confusa.

Martínez Ruiz MG, Richard-Greenblatt M, Juárez ZN, Av-Gay Y, Bach H, Hernández LR - ScientificWorldJournal (2012)

Anti-inflammatory activity of extracts and fractions from Laennecia confusa. An inflammatory process was elicited by exposing macrophages to LPS. Crude extracts were exposed to macrophages before the addition of LPS. The anti-inflammatory process was evaluated by measuring the secretion of IL-6 into the supernatant. Shown is the mean ± SD of three independent experiments. Numbers on the bars indicate the concentration of IL-6 in pg/mL. HEE, CEE, MEE, and AEE are hexane, chloroform, methanol, and aqueous extracts, respectively. *P value < 0.0001.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351095&req=5

fig4: Anti-inflammatory activity of extracts and fractions from Laennecia confusa. An inflammatory process was elicited by exposing macrophages to LPS. Crude extracts were exposed to macrophages before the addition of LPS. The anti-inflammatory process was evaluated by measuring the secretion of IL-6 into the supernatant. Shown is the mean ± SD of three independent experiments. Numbers on the bars indicate the concentration of IL-6 in pg/mL. HEE, CEE, MEE, and AEE are hexane, chloroform, methanol, and aqueous extracts, respectively. *P value < 0.0001.
Mentions: Human-derived macrophages were exposed to each of the extracts and then an inflammatory response was induced with LPS. To evaluate the inflammatory response, the secretion of the pro- and anti-inflammatory IL-6 cytokine was measured in the supernatant of the cultures. A concentration of 580 pg/mL of IL-6 was measured in the supernatant when LPS was added to the macrophages, while levels of 233 and 251 pg/mL were measured when these cells were pretreated with HEE and MEE, respectively (Figure 4). These values represent a reduction of approximately 60% in the secretion of IL-6. Similar results were measured when macrophages were exposed to prednisolone or dexamethasone, suggesting both extracts contains anti-inflammatory compounds. No effects were observed when either the CEE or AEE was supplemented to the cultures.

Bottom Line: The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G.All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages.In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula, PUE, Mexico. luisr.hernandez@udlap.mx

ABSTRACT
The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G. L. Nesom (Asteraceae). Fractions from the hexane, chloroform, methanol, and aqueous extracts were analyzed for antibacterial, antifungal, anti-inflammatory, and antiparasitic activities. The antimicrobial activity of the extracts and fractions was assessed on bacterial and fungal strains, in addition to the protozoa Leishmania donovani, using a microdilution assay. The propensity of the plant's compounds to produce adverse effects on human health was also evaluated using propidium iodine to identify damage to human macrophages. The anti-inflammatory activity of the extracts and fractions was investigated by measuring the secretion of interleukin-6. Chemical analyses demonstrated the presence of flavonoids, cyanogenic and cardiotonic glycosides, saponins, sesquiterpene lactones, and triterpenes in the chloroform extract. A number of extracts and fractions show antibacterial activity. Of particular interest is antibacterial activity against Staphylococcus aureus and its relative methicillin-resistant strain, MRSA. Hexanic and chloroformic fractions also exhibit antifungal activity and two extracts and the fraction CE 2 antiparasitic activity against Leishmania donovani. All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages. In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

Show MeSH
Related in: MedlinePlus