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Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Laennecia confusa.

Martínez Ruiz MG, Richard-Greenblatt M, Juárez ZN, Av-Gay Y, Bach H, Hernández LR - ScientificWorldJournal (2012)

Bottom Line: The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G.All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages.In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula, PUE, Mexico. luisr.hernandez@udlap.mx

ABSTRACT
The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G. L. Nesom (Asteraceae). Fractions from the hexane, chloroform, methanol, and aqueous extracts were analyzed for antibacterial, antifungal, anti-inflammatory, and antiparasitic activities. The antimicrobial activity of the extracts and fractions was assessed on bacterial and fungal strains, in addition to the protozoa Leishmania donovani, using a microdilution assay. The propensity of the plant's compounds to produce adverse effects on human health was also evaluated using propidium iodine to identify damage to human macrophages. The anti-inflammatory activity of the extracts and fractions was investigated by measuring the secretion of interleukin-6. Chemical analyses demonstrated the presence of flavonoids, cyanogenic and cardiotonic glycosides, saponins, sesquiterpene lactones, and triterpenes in the chloroform extract. A number of extracts and fractions show antibacterial activity. Of particular interest is antibacterial activity against Staphylococcus aureus and its relative methicillin-resistant strain, MRSA. Hexanic and chloroformic fractions also exhibit antifungal activity and two extracts and the fraction CE 2 antiparasitic activity against Leishmania donovani. All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages. In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

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Cytotoxic effects of compounds from Laennecia confusa. (a) THP-1 cells were used to assess the cytotoxic effects of bioactive compounds using propidium iodine staining and the obtained values were used to calculate the IC50 (b). Dashed lines represent treatment with 5% H2O2 (upper line) (positive control) and untreated cells (lower line) (negative control). Shown is the mean ± SD of three independent experiments. CE is a chloroformic fraction, and AEE and CEE are aqueous and chloroform extracts, respectively. *P value < 0.0001.
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fig3: Cytotoxic effects of compounds from Laennecia confusa. (a) THP-1 cells were used to assess the cytotoxic effects of bioactive compounds using propidium iodine staining and the obtained values were used to calculate the IC50 (b). Dashed lines represent treatment with 5% H2O2 (upper line) (positive control) and untreated cells (lower line) (negative control). Shown is the mean ± SD of three independent experiments. CE is a chloroformic fraction, and AEE and CEE are aqueous and chloroform extracts, respectively. *P value < 0.0001.

Mentions: The extracts and fractions demonstrating antiparasitic activity in the previous assay were incubated with the human-derived monocyte THP-1 cells to determine their cytotoxic effects. Following 24 h exposure, all of the tested compounds exhibited toxicity to macrophages (Figure 3(a)) with calculated IC50 (±SD) values of 24.8 ± 1.89, 25 ± 2.35, and 24.2 ± 1.23 μg/mL for the AEE and CEE and the chloroformic fraction CE 2, respectively (Figure 3(b)).


Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Laennecia confusa.

Martínez Ruiz MG, Richard-Greenblatt M, Juárez ZN, Av-Gay Y, Bach H, Hernández LR - ScientificWorldJournal (2012)

Cytotoxic effects of compounds from Laennecia confusa. (a) THP-1 cells were used to assess the cytotoxic effects of bioactive compounds using propidium iodine staining and the obtained values were used to calculate the IC50 (b). Dashed lines represent treatment with 5% H2O2 (upper line) (positive control) and untreated cells (lower line) (negative control). Shown is the mean ± SD of three independent experiments. CE is a chloroformic fraction, and AEE and CEE are aqueous and chloroform extracts, respectively. *P value < 0.0001.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351095&req=5

fig3: Cytotoxic effects of compounds from Laennecia confusa. (a) THP-1 cells were used to assess the cytotoxic effects of bioactive compounds using propidium iodine staining and the obtained values were used to calculate the IC50 (b). Dashed lines represent treatment with 5% H2O2 (upper line) (positive control) and untreated cells (lower line) (negative control). Shown is the mean ± SD of three independent experiments. CE is a chloroformic fraction, and AEE and CEE are aqueous and chloroform extracts, respectively. *P value < 0.0001.
Mentions: The extracts and fractions demonstrating antiparasitic activity in the previous assay were incubated with the human-derived monocyte THP-1 cells to determine their cytotoxic effects. Following 24 h exposure, all of the tested compounds exhibited toxicity to macrophages (Figure 3(a)) with calculated IC50 (±SD) values of 24.8 ± 1.89, 25 ± 2.35, and 24.2 ± 1.23 μg/mL for the AEE and CEE and the chloroformic fraction CE 2, respectively (Figure 3(b)).

Bottom Line: The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G.All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages.In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula, PUE, Mexico. luisr.hernandez@udlap.mx

ABSTRACT
The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G. L. Nesom (Asteraceae). Fractions from the hexane, chloroform, methanol, and aqueous extracts were analyzed for antibacterial, antifungal, anti-inflammatory, and antiparasitic activities. The antimicrobial activity of the extracts and fractions was assessed on bacterial and fungal strains, in addition to the protozoa Leishmania donovani, using a microdilution assay. The propensity of the plant's compounds to produce adverse effects on human health was also evaluated using propidium iodine to identify damage to human macrophages. The anti-inflammatory activity of the extracts and fractions was investigated by measuring the secretion of interleukin-6. Chemical analyses demonstrated the presence of flavonoids, cyanogenic and cardiotonic glycosides, saponins, sesquiterpene lactones, and triterpenes in the chloroform extract. A number of extracts and fractions show antibacterial activity. Of particular interest is antibacterial activity against Staphylococcus aureus and its relative methicillin-resistant strain, MRSA. Hexanic and chloroformic fractions also exhibit antifungal activity and two extracts and the fraction CE 2 antiparasitic activity against Leishmania donovani. All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages. In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

Show MeSH
Related in: MedlinePlus