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Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Laennecia confusa.

Martínez Ruiz MG, Richard-Greenblatt M, Juárez ZN, Av-Gay Y, Bach H, Hernández LR - ScientificWorldJournal (2012)

Bottom Line: The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G.All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages.In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula, PUE, Mexico. luisr.hernandez@udlap.mx

ABSTRACT
The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G. L. Nesom (Asteraceae). Fractions from the hexane, chloroform, methanol, and aqueous extracts were analyzed for antibacterial, antifungal, anti-inflammatory, and antiparasitic activities. The antimicrobial activity of the extracts and fractions was assessed on bacterial and fungal strains, in addition to the protozoa Leishmania donovani, using a microdilution assay. The propensity of the plant's compounds to produce adverse effects on human health was also evaluated using propidium iodine to identify damage to human macrophages. The anti-inflammatory activity of the extracts and fractions was investigated by measuring the secretion of interleukin-6. Chemical analyses demonstrated the presence of flavonoids, cyanogenic and cardiotonic glycosides, saponins, sesquiterpene lactones, and triterpenes in the chloroform extract. A number of extracts and fractions show antibacterial activity. Of particular interest is antibacterial activity against Staphylococcus aureus and its relative methicillin-resistant strain, MRSA. Hexanic and chloroformic fractions also exhibit antifungal activity and two extracts and the fraction CE 2 antiparasitic activity against Leishmania donovani. All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages. In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

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Antiparasitic activity exhibited by fractions of Laennecia confusa. Leishmania donovani promastigote growth inhibition was evaluated after incubation of the parasites with the compounds. Untreated promastigotes and DMSO were used as negative controls. Control: untreated promastigotes. Shown is the mean ± SD of three independent experiments. *P value < 0.0001.
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fig2: Antiparasitic activity exhibited by fractions of Laennecia confusa. Leishmania donovani promastigote growth inhibition was evaluated after incubation of the parasites with the compounds. Untreated promastigotes and DMSO were used as negative controls. Control: untreated promastigotes. Shown is the mean ± SD of three independent experiments. *P value < 0.0001.

Mentions: A reduction in growth of L. donovani in comparison to the controls from the aqueous and chloroform extracts and the chloroformic fraction CE 2 was observed in the antiparasitic assay. A decrease of approximately 50% in the number of parasites was measured after 72 h after exposure of the tested compounds (Figure 2). IC50 values of 20 μg/mL were calculated for both aqueous and chloroform extracts, whereas an IC50 of 200 μg/mL was measured for the chloroformic fraction CE 2 (Figure 2).


Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Laennecia confusa.

Martínez Ruiz MG, Richard-Greenblatt M, Juárez ZN, Av-Gay Y, Bach H, Hernández LR - ScientificWorldJournal (2012)

Antiparasitic activity exhibited by fractions of Laennecia confusa. Leishmania donovani promastigote growth inhibition was evaluated after incubation of the parasites with the compounds. Untreated promastigotes and DMSO were used as negative controls. Control: untreated promastigotes. Shown is the mean ± SD of three independent experiments. *P value < 0.0001.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351095&req=5

fig2: Antiparasitic activity exhibited by fractions of Laennecia confusa. Leishmania donovani promastigote growth inhibition was evaluated after incubation of the parasites with the compounds. Untreated promastigotes and DMSO were used as negative controls. Control: untreated promastigotes. Shown is the mean ± SD of three independent experiments. *P value < 0.0001.
Mentions: A reduction in growth of L. donovani in comparison to the controls from the aqueous and chloroform extracts and the chloroformic fraction CE 2 was observed in the antiparasitic assay. A decrease of approximately 50% in the number of parasites was measured after 72 h after exposure of the tested compounds (Figure 2). IC50 values of 20 μg/mL were calculated for both aqueous and chloroform extracts, whereas an IC50 of 200 μg/mL was measured for the chloroformic fraction CE 2 (Figure 2).

Bottom Line: The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G.All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages.In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula, PUE, Mexico. luisr.hernandez@udlap.mx

ABSTRACT
The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G. L. Nesom (Asteraceae). Fractions from the hexane, chloroform, methanol, and aqueous extracts were analyzed for antibacterial, antifungal, anti-inflammatory, and antiparasitic activities. The antimicrobial activity of the extracts and fractions was assessed on bacterial and fungal strains, in addition to the protozoa Leishmania donovani, using a microdilution assay. The propensity of the plant's compounds to produce adverse effects on human health was also evaluated using propidium iodine to identify damage to human macrophages. The anti-inflammatory activity of the extracts and fractions was investigated by measuring the secretion of interleukin-6. Chemical analyses demonstrated the presence of flavonoids, cyanogenic and cardiotonic glycosides, saponins, sesquiterpene lactones, and triterpenes in the chloroform extract. A number of extracts and fractions show antibacterial activity. Of particular interest is antibacterial activity against Staphylococcus aureus and its relative methicillin-resistant strain, MRSA. Hexanic and chloroformic fractions also exhibit antifungal activity and two extracts and the fraction CE 2 antiparasitic activity against Leishmania donovani. All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages. In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

Show MeSH
Related in: MedlinePlus