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Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Laennecia confusa.

Martínez Ruiz MG, Richard-Greenblatt M, Juárez ZN, Av-Gay Y, Bach H, Hernández LR - ScientificWorldJournal (2012)

Bottom Line: The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G.All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages.In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula, PUE, Mexico. luisr.hernandez@udlap.mx

ABSTRACT
The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G. L. Nesom (Asteraceae). Fractions from the hexane, chloroform, methanol, and aqueous extracts were analyzed for antibacterial, antifungal, anti-inflammatory, and antiparasitic activities. The antimicrobial activity of the extracts and fractions was assessed on bacterial and fungal strains, in addition to the protozoa Leishmania donovani, using a microdilution assay. The propensity of the plant's compounds to produce adverse effects on human health was also evaluated using propidium iodine to identify damage to human macrophages. The anti-inflammatory activity of the extracts and fractions was investigated by measuring the secretion of interleukin-6. Chemical analyses demonstrated the presence of flavonoids, cyanogenic and cardiotonic glycosides, saponins, sesquiterpene lactones, and triterpenes in the chloroform extract. A number of extracts and fractions show antibacterial activity. Of particular interest is antibacterial activity against Staphylococcus aureus and its relative methicillin-resistant strain, MRSA. Hexanic and chloroformic fractions also exhibit antifungal activity and two extracts and the fraction CE 2 antiparasitic activity against Leishmania donovani. All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages. In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

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Related in: MedlinePlus

Diagram showing the fractionation of the hexane, chloroform, methanol, and aqueous extracts extracted from the aerial parts of Laennecia confusa. Ratios of the combined solvents are expressed in volume/volume.
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fig1: Diagram showing the fractionation of the hexane, chloroform, methanol, and aqueous extracts extracted from the aerial parts of Laennecia confusa. Ratios of the combined solvents are expressed in volume/volume.

Mentions: A total of 200 g of air-dried aerial parts of L. confusa were sequentially extracted with n-hexane (hexane), chloroform, and methanol, after macerating the material in each solvent in three rounds for 48 h at room temperature. To simulate a herb infusion (according to the traditional preparation), an aqueous extraction was carried out by soaking 200 g of the same plant material in boiled distilled water for 2 h. Following filtering, solvent volatilization, and lyophilization (for aqueous extract), the extracts were chromatographed on silica gel (70–230 mesh) using approximately 300 mL of pure or combined solvents according to Figure 1. Fractions were dried in a vacuum using a Rotavapor and stock solutions were prepared by dissolving 20 mg of each fraction in 100 μL DMSO (or equivalent to obtain the same final amount), following sonication (Branson 3210) for 60 min at 30°C until the material was completely dissolved.


Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Laennecia confusa.

Martínez Ruiz MG, Richard-Greenblatt M, Juárez ZN, Av-Gay Y, Bach H, Hernández LR - ScientificWorldJournal (2012)

Diagram showing the fractionation of the hexane, chloroform, methanol, and aqueous extracts extracted from the aerial parts of Laennecia confusa. Ratios of the combined solvents are expressed in volume/volume.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351095&req=5

fig1: Diagram showing the fractionation of the hexane, chloroform, methanol, and aqueous extracts extracted from the aerial parts of Laennecia confusa. Ratios of the combined solvents are expressed in volume/volume.
Mentions: A total of 200 g of air-dried aerial parts of L. confusa were sequentially extracted with n-hexane (hexane), chloroform, and methanol, after macerating the material in each solvent in three rounds for 48 h at room temperature. To simulate a herb infusion (according to the traditional preparation), an aqueous extraction was carried out by soaking 200 g of the same plant material in boiled distilled water for 2 h. Following filtering, solvent volatilization, and lyophilization (for aqueous extract), the extracts were chromatographed on silica gel (70–230 mesh) using approximately 300 mL of pure or combined solvents according to Figure 1. Fractions were dried in a vacuum using a Rotavapor and stock solutions were prepared by dissolving 20 mg of each fraction in 100 μL DMSO (or equivalent to obtain the same final amount), following sonication (Branson 3210) for 60 min at 30°C until the material was completely dissolved.

Bottom Line: The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G.All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages.In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciencias Químico-Biológicas, Universidad de las Américas Puebla, Santa Catarina Mártir s/n, Cholula, PUE, Mexico. luisr.hernandez@udlap.mx

ABSTRACT
The current paper investigated the potential benefit of the traditional Mexican medicinal plant Laennecia confusa (Cronquist) G. L. Nesom (Asteraceae). Fractions from the hexane, chloroform, methanol, and aqueous extracts were analyzed for antibacterial, antifungal, anti-inflammatory, and antiparasitic activities. The antimicrobial activity of the extracts and fractions was assessed on bacterial and fungal strains, in addition to the protozoa Leishmania donovani, using a microdilution assay. The propensity of the plant's compounds to produce adverse effects on human health was also evaluated using propidium iodine to identify damage to human macrophages. The anti-inflammatory activity of the extracts and fractions was investigated by measuring the secretion of interleukin-6. Chemical analyses demonstrated the presence of flavonoids, cyanogenic and cardiotonic glycosides, saponins, sesquiterpene lactones, and triterpenes in the chloroform extract. A number of extracts and fractions show antibacterial activity. Of particular interest is antibacterial activity against Staphylococcus aureus and its relative methicillin-resistant strain, MRSA. Hexanic and chloroformic fractions also exhibit antifungal activity and two extracts and the fraction CE 2 antiparasitic activity against Leishmania donovani. All bioactive extracts and fractions assayed were also found to be cytotoxic to macrophages. In addition, the hexane and methane extracts show anti-inflammatory activity by suppressing the secretion of interleukine-6.

Show MeSH
Related in: MedlinePlus