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Molecular markers of dental pulp tissue during orthodontic tooth movement: a pilot study.

Abdul Wahab RM, Zainal Ariffin SH, Yeen WW, Ahmad NA, Senafi S - ScientificWorldJournal (2012)

Bottom Line: Three specific orthodontic tooth movement genes, that is, FCRL1, HSPG2, and LAMB2 were detected at upper first premolar (with appliance) dental pulp tissue by using GeneFishing technique as compared to lower first premolar (without appliance).These three differentially expressed genes have the potential as molecular markers during orthodontic tooth movement by looking at molecular changes of pulp tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthodontics, Faculty of Dentistry, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia.

ABSTRACT
Three specific orthodontic tooth movement genes, that is, FCRL1, HSPG2, and LAMB2 were detected at upper first premolar (with appliance) dental pulp tissue by using GeneFishing technique as compared to lower first premolar (without appliance). These three differentially expressed genes have the potential as molecular markers during orthodontic tooth movement by looking at molecular changes of pulp tissue.

Show MeSH
GeneFishing DEG screening results. There are 5 genes activated in pulp tissue (arrows). Lane 1: Normal; Lane 2: 14 days after force application; Lane 3: 28 days after force application; Lane M: 100 bp DNA marker.
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fig1: GeneFishing DEG screening results. There are 5 genes activated in pulp tissue (arrows). Lane 1: Normal; Lane 2: 14 days after force application; Lane 3: 28 days after force application; Lane M: 100 bp DNA marker.

Mentions: The mRNA expression profiles of extracted teeth on the 14th and 28th days after orthodontic treatment were compared with normal pulp tissue to identify genes that specifically or predominantly expressed in pulp tissue during OTM. The mRNA from both types of tissues was extracted and subjected to ACP 1-20 RT-PCR analysis. The expression pattern of differentially expressed transcripts was obtained only when subjected to ACPs 5, 7, 8, 9, and 10 in response to the orthodontic treatment of various periods, that is, days 0, 14, and 28 as shown in Table 1 and Figure 1. All of the DEGs obtained were cloned, sequenced, and confirmed with GenBank. The sequence similarities of differentially expressed transcripts were analyzed using BLASTN analysis (http://www.ncbi.nlm.nih.gov/). ACPs 5, 7, and 8 which were used in this study, encoded the same gene expression before and after treatment except for ACPs 9 and 10 (Table 1; Figure 1).


Molecular markers of dental pulp tissue during orthodontic tooth movement: a pilot study.

Abdul Wahab RM, Zainal Ariffin SH, Yeen WW, Ahmad NA, Senafi S - ScientificWorldJournal (2012)

GeneFishing DEG screening results. There are 5 genes activated in pulp tissue (arrows). Lane 1: Normal; Lane 2: 14 days after force application; Lane 3: 28 days after force application; Lane M: 100 bp DNA marker.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3351084&req=5

fig1: GeneFishing DEG screening results. There are 5 genes activated in pulp tissue (arrows). Lane 1: Normal; Lane 2: 14 days after force application; Lane 3: 28 days after force application; Lane M: 100 bp DNA marker.
Mentions: The mRNA expression profiles of extracted teeth on the 14th and 28th days after orthodontic treatment were compared with normal pulp tissue to identify genes that specifically or predominantly expressed in pulp tissue during OTM. The mRNA from both types of tissues was extracted and subjected to ACP 1-20 RT-PCR analysis. The expression pattern of differentially expressed transcripts was obtained only when subjected to ACPs 5, 7, 8, 9, and 10 in response to the orthodontic treatment of various periods, that is, days 0, 14, and 28 as shown in Table 1 and Figure 1. All of the DEGs obtained were cloned, sequenced, and confirmed with GenBank. The sequence similarities of differentially expressed transcripts were analyzed using BLASTN analysis (http://www.ncbi.nlm.nih.gov/). ACPs 5, 7, and 8 which were used in this study, encoded the same gene expression before and after treatment except for ACPs 9 and 10 (Table 1; Figure 1).

Bottom Line: Three specific orthodontic tooth movement genes, that is, FCRL1, HSPG2, and LAMB2 were detected at upper first premolar (with appliance) dental pulp tissue by using GeneFishing technique as compared to lower first premolar (without appliance).These three differentially expressed genes have the potential as molecular markers during orthodontic tooth movement by looking at molecular changes of pulp tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthodontics, Faculty of Dentistry, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia.

ABSTRACT
Three specific orthodontic tooth movement genes, that is, FCRL1, HSPG2, and LAMB2 were detected at upper first premolar (with appliance) dental pulp tissue by using GeneFishing technique as compared to lower first premolar (without appliance). These three differentially expressed genes have the potential as molecular markers during orthodontic tooth movement by looking at molecular changes of pulp tissue.

Show MeSH