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Analysis of In Vitro Effects of Sex Steroids on Lymphocyte Responsiveness in Murrah Buffaloes (Bubalus bubalis).

Pampori ZA, Pandita S - Vet Med Int (2012)

Bottom Line: Present study was carried out on forty four apparently healthy Murrah buffaloes of different age groups of both sexes to investigate the effects of sex steroids on cell mediated immunity in vitro.Significantly high levels of NO in culture supernatant were found in prepubertal buffalo calves and least in post pubertal buffaloes, which had an inverse relation with lymphocyte proliferation in presence of estradiol.No significant difference in NO culture supernatant was observed between different concentrations of testosterone treatment.

View Article: PubMed Central - PubMed

Affiliation: Dairy Cattle Physiology Division, National Dairy Research Institute (ICAR), Haryana, Karnal 132001, India.

ABSTRACT
Present study was carried out on forty four apparently healthy Murrah buffaloes of different age groups of both sexes to investigate the effects of sex steroids on cell mediated immunity in vitro. Estrogen inhibited proliferation in mitogen-stimulated lymphocytes from prepubertal but not post pubertal buffaloes of either sex. Estrogen at 100 pg/mL concentration stimulating the proliferation significantly (P < 0.05). in all groups and had higher stimulatory effect in lymphocytes from day 10 than day 0 of estrous cycle. Progesterone inhibited lymphocyte proliferation, and inhibition was directly related to the dose, in all groups of either sex. Testosterone did not inhibit proliferation at any dose level and did not show any consistent and lucid effects on lymphocyte proliferation. Present study revealed that buffalo lymphocytes produce appreciable amounts of NO in culture system after treatment with estradiol. Significantly high levels of NO in culture supernatant were found in prepubertal buffalo calves and least in post pubertal buffaloes, which had an inverse relation with lymphocyte proliferation in presence of estradiol. NO in culture supernatant was high at the lowest dose of progesterone which was proportional to the lymphocyte proliferation when treated with progesterone. No significant difference in NO culture supernatant was observed between different concentrations of testosterone treatment.

No MeSH data available.


NO in culture supernatant supplemented with different levels of progesterone in Murrah buffaloes.
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fig5: NO in culture supernatant supplemented with different levels of progesterone in Murrah buffaloes.

Mentions: Significant (P < 0.001) difference in levels of nitric oxide in culture supernatant supplemented with different concentrations of progesterone was registered between the sexes (6.58 ± 0.23 and 4.97 ± 0.23 μM/L in males and females resp.) and between the age groups (4.50 ± 0.28, 6.56 ± 0.28, and 6.24 ± 0.28 μM/L in group-I, group-II, & group-III buffaloes, resp.). Among the groups, group-II males registered highest (P < 0.05) levels of NO in culture supernatant where as group-I females the lowest (Figure 4). Similarly nitric oxide levels in culture supernatant differed significantly (P < 0.01) between various concentrations of progesterone treatment (Figure 5).


Analysis of In Vitro Effects of Sex Steroids on Lymphocyte Responsiveness in Murrah Buffaloes (Bubalus bubalis).

Pampori ZA, Pandita S - Vet Med Int (2012)

NO in culture supernatant supplemented with different levels of progesterone in Murrah buffaloes.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3350872&req=5

fig5: NO in culture supernatant supplemented with different levels of progesterone in Murrah buffaloes.
Mentions: Significant (P < 0.001) difference in levels of nitric oxide in culture supernatant supplemented with different concentrations of progesterone was registered between the sexes (6.58 ± 0.23 and 4.97 ± 0.23 μM/L in males and females resp.) and between the age groups (4.50 ± 0.28, 6.56 ± 0.28, and 6.24 ± 0.28 μM/L in group-I, group-II, & group-III buffaloes, resp.). Among the groups, group-II males registered highest (P < 0.05) levels of NO in culture supernatant where as group-I females the lowest (Figure 4). Similarly nitric oxide levels in culture supernatant differed significantly (P < 0.01) between various concentrations of progesterone treatment (Figure 5).

Bottom Line: Present study was carried out on forty four apparently healthy Murrah buffaloes of different age groups of both sexes to investigate the effects of sex steroids on cell mediated immunity in vitro.Significantly high levels of NO in culture supernatant were found in prepubertal buffalo calves and least in post pubertal buffaloes, which had an inverse relation with lymphocyte proliferation in presence of estradiol.No significant difference in NO culture supernatant was observed between different concentrations of testosterone treatment.

View Article: PubMed Central - PubMed

Affiliation: Dairy Cattle Physiology Division, National Dairy Research Institute (ICAR), Haryana, Karnal 132001, India.

ABSTRACT
Present study was carried out on forty four apparently healthy Murrah buffaloes of different age groups of both sexes to investigate the effects of sex steroids on cell mediated immunity in vitro. Estrogen inhibited proliferation in mitogen-stimulated lymphocytes from prepubertal but not post pubertal buffaloes of either sex. Estrogen at 100 pg/mL concentration stimulating the proliferation significantly (P < 0.05). in all groups and had higher stimulatory effect in lymphocytes from day 10 than day 0 of estrous cycle. Progesterone inhibited lymphocyte proliferation, and inhibition was directly related to the dose, in all groups of either sex. Testosterone did not inhibit proliferation at any dose level and did not show any consistent and lucid effects on lymphocyte proliferation. Present study revealed that buffalo lymphocytes produce appreciable amounts of NO in culture system after treatment with estradiol. Significantly high levels of NO in culture supernatant were found in prepubertal buffalo calves and least in post pubertal buffaloes, which had an inverse relation with lymphocyte proliferation in presence of estradiol. NO in culture supernatant was high at the lowest dose of progesterone which was proportional to the lymphocyte proliferation when treated with progesterone. No significant difference in NO culture supernatant was observed between different concentrations of testosterone treatment.

No MeSH data available.