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Analysis of In Vitro Effects of Sex Steroids on Lymphocyte Responsiveness in Murrah Buffaloes (Bubalus bubalis).

Pampori ZA, Pandita S - Vet Med Int (2012)

Bottom Line: Present study was carried out on forty four apparently healthy Murrah buffaloes of different age groups of both sexes to investigate the effects of sex steroids on cell mediated immunity in vitro.Significantly high levels of NO in culture supernatant were found in prepubertal buffalo calves and least in post pubertal buffaloes, which had an inverse relation with lymphocyte proliferation in presence of estradiol.No significant difference in NO culture supernatant was observed between different concentrations of testosterone treatment.

View Article: PubMed Central - PubMed

Affiliation: Dairy Cattle Physiology Division, National Dairy Research Institute (ICAR), Haryana, Karnal 132001, India.

ABSTRACT
Present study was carried out on forty four apparently healthy Murrah buffaloes of different age groups of both sexes to investigate the effects of sex steroids on cell mediated immunity in vitro. Estrogen inhibited proliferation in mitogen-stimulated lymphocytes from prepubertal but not post pubertal buffaloes of either sex. Estrogen at 100 pg/mL concentration stimulating the proliferation significantly (P < 0.05). in all groups and had higher stimulatory effect in lymphocytes from day 10 than day 0 of estrous cycle. Progesterone inhibited lymphocyte proliferation, and inhibition was directly related to the dose, in all groups of either sex. Testosterone did not inhibit proliferation at any dose level and did not show any consistent and lucid effects on lymphocyte proliferation. Present study revealed that buffalo lymphocytes produce appreciable amounts of NO in culture system after treatment with estradiol. Significantly high levels of NO in culture supernatant were found in prepubertal buffalo calves and least in post pubertal buffaloes, which had an inverse relation with lymphocyte proliferation in presence of estradiol. NO in culture supernatant was high at the lowest dose of progesterone which was proportional to the lymphocyte proliferation when treated with progesterone. No significant difference in NO culture supernatant was observed between different concentrations of testosterone treatment.

No MeSH data available.


NO culture supernatant in Murrah buffaloes of different sex, age, and stage of cycle.
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Related In: Results  -  Collection


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fig1: NO culture supernatant in Murrah buffaloes of different sex, age, and stage of cycle.

Mentions: Nitric oxide was evaluated in supernatant collected 36 hours after lymphocyte culture (Figure 1). Males produced significantly (P < 0.001) higher (8.52 ± 0.39 μM/L) NO in culture than females (6.13 ± 0.39 μM/L). The difference was also significant (P < 0.05) among the three groups with lowest in group-III (6.23 ± 0.48 μM/L), followed by group-II (8.07 ± 0.48 μM/L) and group-I (7.68 ± 0.48 μM/L). Among all groups, group-II male buffaloes registered the highest (9.89 ± 1.18 μM/L) and group-III female buffaloes the lowest (4.87 ± 0.42 μM/L) levels of nitric oxide in culture supernatant. In cyclic buffaloes the nitric oxide concentration in culture supernatant did not differ significantly with the stage of estrous cycle and was 5.20 ± 0.29 and 4.88 ± 0.41 μM/L on day 0 (estrus) and day 10 (diestrus), respectively (Figure 1).


Analysis of In Vitro Effects of Sex Steroids on Lymphocyte Responsiveness in Murrah Buffaloes (Bubalus bubalis).

Pampori ZA, Pandita S - Vet Med Int (2012)

NO culture supernatant in Murrah buffaloes of different sex, age, and stage of cycle.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3350872&req=5

fig1: NO culture supernatant in Murrah buffaloes of different sex, age, and stage of cycle.
Mentions: Nitric oxide was evaluated in supernatant collected 36 hours after lymphocyte culture (Figure 1). Males produced significantly (P < 0.001) higher (8.52 ± 0.39 μM/L) NO in culture than females (6.13 ± 0.39 μM/L). The difference was also significant (P < 0.05) among the three groups with lowest in group-III (6.23 ± 0.48 μM/L), followed by group-II (8.07 ± 0.48 μM/L) and group-I (7.68 ± 0.48 μM/L). Among all groups, group-II male buffaloes registered the highest (9.89 ± 1.18 μM/L) and group-III female buffaloes the lowest (4.87 ± 0.42 μM/L) levels of nitric oxide in culture supernatant. In cyclic buffaloes the nitric oxide concentration in culture supernatant did not differ significantly with the stage of estrous cycle and was 5.20 ± 0.29 and 4.88 ± 0.41 μM/L on day 0 (estrus) and day 10 (diestrus), respectively (Figure 1).

Bottom Line: Present study was carried out on forty four apparently healthy Murrah buffaloes of different age groups of both sexes to investigate the effects of sex steroids on cell mediated immunity in vitro.Significantly high levels of NO in culture supernatant were found in prepubertal buffalo calves and least in post pubertal buffaloes, which had an inverse relation with lymphocyte proliferation in presence of estradiol.No significant difference in NO culture supernatant was observed between different concentrations of testosterone treatment.

View Article: PubMed Central - PubMed

Affiliation: Dairy Cattle Physiology Division, National Dairy Research Institute (ICAR), Haryana, Karnal 132001, India.

ABSTRACT
Present study was carried out on forty four apparently healthy Murrah buffaloes of different age groups of both sexes to investigate the effects of sex steroids on cell mediated immunity in vitro. Estrogen inhibited proliferation in mitogen-stimulated lymphocytes from prepubertal but not post pubertal buffaloes of either sex. Estrogen at 100 pg/mL concentration stimulating the proliferation significantly (P < 0.05). in all groups and had higher stimulatory effect in lymphocytes from day 10 than day 0 of estrous cycle. Progesterone inhibited lymphocyte proliferation, and inhibition was directly related to the dose, in all groups of either sex. Testosterone did not inhibit proliferation at any dose level and did not show any consistent and lucid effects on lymphocyte proliferation. Present study revealed that buffalo lymphocytes produce appreciable amounts of NO in culture system after treatment with estradiol. Significantly high levels of NO in culture supernatant were found in prepubertal buffalo calves and least in post pubertal buffaloes, which had an inverse relation with lymphocyte proliferation in presence of estradiol. NO in culture supernatant was high at the lowest dose of progesterone which was proportional to the lymphocyte proliferation when treated with progesterone. No significant difference in NO culture supernatant was observed between different concentrations of testosterone treatment.

No MeSH data available.