Limits...
Expression and immunolocalization of Gpnmb, a glioma-associated glycoprotein, in normal and inflamed central nervous systems of adult rats.

Huang JJ, Ma WJ, Yokoyama S - Brain Behav (2012)

Bottom Line: Double immunofluorescence staining showed that these cells were co-stained most frequently with the microglia/macrophage marker OX42, and occasionally with the radial glia marker RC2 or the neuronal marker NeuN.Furthermore, an intraperitoneal injection of bacterial endotoxin lipopolysaccharide increased the number of Gpnmb and OX42 double-positive cells in the area postrema, which is one of the circumventricular organs, indicating infiltration of hematogenous macrophages.These results suggest that Gpnmb, which is expressed in microglia and macrophages in non-tumorous neural tissues, plays an important role in the regulation of immune/inflammatory responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Biophysical Genetics, Kanazawa University Graduate School of Medicine Kanazawa 920-8640, Japan.

ABSTRACT
Glycoprotein nonmetastatic melanoma B (Gpnmb) is a type I transmembrane protein implicated in cell differentiation, inflammation, tissue regeneration, and tumor progression. Gpnmb, which is highly expressed in glioblastoma cells, is a potential therapeutic target. However, little is known about its expression, cellular localization, and roles in non-tumorous neural tissues. In this study, we examined Gpnmb expression in the central nervous system of adult rats under both normal and inflammatory conditions. Reverse transcription-polymerase chain reaction analysis revealed that Gpnmb mRNA was expressed in the cerebrum, cerebellum, brain stem, and spinal cord of normal adult rats. Immunoperoxidase staining revealed that Gpnmb-immunoreactive cells were widely distributed in the parenchyma of all brain regions examined, with the cells being most prevalent in the hippocampal dentate gyrus, cerebellar cortex, spinal dorsal horn, choroid plexus, ependyma, periventricular regions, and in layers II and III of the cerebral cortex. Double immunofluorescence staining showed that these cells were co-stained most frequently with the microglia/macrophage marker OX42, and occasionally with the radial glia marker RC2 or the neuronal marker NeuN. Furthermore, an intraperitoneal injection of bacterial endotoxin lipopolysaccharide increased the number of Gpnmb and OX42 double-positive cells in the area postrema, which is one of the circumventricular organs, indicating infiltration of hematogenous macrophages. These results suggest that Gpnmb, which is expressed in microglia and macrophages in non-tumorous neural tissues, plays an important role in the regulation of immune/inflammatory responses.

No MeSH data available.


Related in: MedlinePlus

Characterization of Gpnmb-IR cells in cortical layers II–III with multiple markers. Sections were double-stained for Gpnmb (FITC, green) and the indicated markers (Texas Red, red). Note that Gpnmb-IR cells are co-stained with OX42, IB4, and RC2 (arrows). Scale bars: A, 10 μm; B–F, 30 μm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3345354&req=5

fig04: Characterization of Gpnmb-IR cells in cortical layers II–III with multiple markers. Sections were double-stained for Gpnmb (FITC, green) and the indicated markers (Texas Red, red). Note that Gpnmb-IR cells are co-stained with OX42, IB4, and RC2 (arrows). Scale bars: A, 10 μm; B–F, 30 μm.

Mentions: Gpnmb-IR was observed in all layers of the cerebral cortex, but staining was most prominent in layers II and III (Fig. 3A). IR was abolished by preadsorption of the primary antibody with the peptide used for immunization (Fig. 3B). Some of the Gpnmb-IR cells appeared to have fine processes (Fig. 3C, D). Double immunofluorescence staining revealed that Gpnmb-IR cells in layers II and III were positive for the microglia/macrophage markers OX42 (Fig. 4A) and IB4 (Fig. 4B). Although less frequently, some Gpnmb-IR cells were co-stained for the radial glial lineage marker RC2 (Fig. 4C). In contrast, no co-staining was observed with antibodies to the astrocyte lineage markers glial fibrillary acidic protein (GFAP; Fig. 4D) and protein S-100β (Fig. 4E) and the neuronal marker NeuN (Fig. 4F). This tendency was the same in other layers, except that occasional co-staining with NeuN was detected in layer VI (Fig. S1).


Expression and immunolocalization of Gpnmb, a glioma-associated glycoprotein, in normal and inflamed central nervous systems of adult rats.

Huang JJ, Ma WJ, Yokoyama S - Brain Behav (2012)

Characterization of Gpnmb-IR cells in cortical layers II–III with multiple markers. Sections were double-stained for Gpnmb (FITC, green) and the indicated markers (Texas Red, red). Note that Gpnmb-IR cells are co-stained with OX42, IB4, and RC2 (arrows). Scale bars: A, 10 μm; B–F, 30 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3345354&req=5

fig04: Characterization of Gpnmb-IR cells in cortical layers II–III with multiple markers. Sections were double-stained for Gpnmb (FITC, green) and the indicated markers (Texas Red, red). Note that Gpnmb-IR cells are co-stained with OX42, IB4, and RC2 (arrows). Scale bars: A, 10 μm; B–F, 30 μm.
Mentions: Gpnmb-IR was observed in all layers of the cerebral cortex, but staining was most prominent in layers II and III (Fig. 3A). IR was abolished by preadsorption of the primary antibody with the peptide used for immunization (Fig. 3B). Some of the Gpnmb-IR cells appeared to have fine processes (Fig. 3C, D). Double immunofluorescence staining revealed that Gpnmb-IR cells in layers II and III were positive for the microglia/macrophage markers OX42 (Fig. 4A) and IB4 (Fig. 4B). Although less frequently, some Gpnmb-IR cells were co-stained for the radial glial lineage marker RC2 (Fig. 4C). In contrast, no co-staining was observed with antibodies to the astrocyte lineage markers glial fibrillary acidic protein (GFAP; Fig. 4D) and protein S-100β (Fig. 4E) and the neuronal marker NeuN (Fig. 4F). This tendency was the same in other layers, except that occasional co-staining with NeuN was detected in layer VI (Fig. S1).

Bottom Line: Double immunofluorescence staining showed that these cells were co-stained most frequently with the microglia/macrophage marker OX42, and occasionally with the radial glia marker RC2 or the neuronal marker NeuN.Furthermore, an intraperitoneal injection of bacterial endotoxin lipopolysaccharide increased the number of Gpnmb and OX42 double-positive cells in the area postrema, which is one of the circumventricular organs, indicating infiltration of hematogenous macrophages.These results suggest that Gpnmb, which is expressed in microglia and macrophages in non-tumorous neural tissues, plays an important role in the regulation of immune/inflammatory responses.

View Article: PubMed Central - PubMed

Affiliation: Department of Biophysical Genetics, Kanazawa University Graduate School of Medicine Kanazawa 920-8640, Japan.

ABSTRACT
Glycoprotein nonmetastatic melanoma B (Gpnmb) is a type I transmembrane protein implicated in cell differentiation, inflammation, tissue regeneration, and tumor progression. Gpnmb, which is highly expressed in glioblastoma cells, is a potential therapeutic target. However, little is known about its expression, cellular localization, and roles in non-tumorous neural tissues. In this study, we examined Gpnmb expression in the central nervous system of adult rats under both normal and inflammatory conditions. Reverse transcription-polymerase chain reaction analysis revealed that Gpnmb mRNA was expressed in the cerebrum, cerebellum, brain stem, and spinal cord of normal adult rats. Immunoperoxidase staining revealed that Gpnmb-immunoreactive cells were widely distributed in the parenchyma of all brain regions examined, with the cells being most prevalent in the hippocampal dentate gyrus, cerebellar cortex, spinal dorsal horn, choroid plexus, ependyma, periventricular regions, and in layers II and III of the cerebral cortex. Double immunofluorescence staining showed that these cells were co-stained most frequently with the microglia/macrophage marker OX42, and occasionally with the radial glia marker RC2 or the neuronal marker NeuN. Furthermore, an intraperitoneal injection of bacterial endotoxin lipopolysaccharide increased the number of Gpnmb and OX42 double-positive cells in the area postrema, which is one of the circumventricular organs, indicating infiltration of hematogenous macrophages. These results suggest that Gpnmb, which is expressed in microglia and macrophages in non-tumorous neural tissues, plays an important role in the regulation of immune/inflammatory responses.

No MeSH data available.


Related in: MedlinePlus