Limits...
Camel milk modulates the expression of aryl hydrocarbon receptor-regulated genes, Cyp1a1, Nqo1, and Gsta1, in murine hepatoma Hepa 1c1c7 cells.

Korashy HM, El Gendy MA, Alhaider AA, El-Kadi AO - J. Biomed. Biotechnol. (2012)

Bottom Line: In addition, camel milk significantly decreased the xenobiotic responsive element (XRE)-dependent luciferase activity, suggesting a transcriptional mechanism is involved.Furthermore, this inhibitory effect of camel milk was associated with a proportional increase in heme oxygenase 1.In conclusion, camel milk modulates the expression of Cyp1a1, Nqo1, and Gsta1 at the transcriptional and posttranscriptional levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, 11451 Riyadh, Saudi Arabia.

ABSTRACT
There is a traditional belief in the Middle East that camel milk may aid in prevention and treatment of numerous cases of cancer yet, the exact mechanism was not investigated. Therefore, we examined the ability of camel milk to modulate the expression of a well-known cancer-activating gene, Cytochrome P450 1a1 (Cyp1a1), and cancer-protective genes, NAD(P)H:quinone oxidoreductase 1 (Nqo1) and glutathione S-transferase a1 (Gsta1), in murine hepatoma Hepa 1c1c7 cell line. Our results showed that camel milk significantly inhibited the induction of Cyp1a1 gene expression by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the most potent Cyp1a1 inducer and known carcinogenic chemical, at mRNA, protein, and activity levels in a concentration-dependent manner. In addition, camel milk significantly decreased the xenobiotic responsive element (XRE)-dependent luciferase activity, suggesting a transcriptional mechanism is involved. Furthermore, this inhibitory effect of camel milk was associated with a proportional increase in heme oxygenase 1. On the other hand, camel milk significantly induced Nqo1 and Gsta1 mRNA expression level in a concentration-dependent fashion. The RNA synthesis inhibitor, actinomycin D, completely blocked the induction of Nqo1 mRNA by camel milk suggesting the requirement of de novo RNA synthesis through a transcriptional mechanism. In conclusion, camel milk modulates the expression of Cyp1a1, Nqo1, and Gsta1 at the transcriptional and posttranscriptional levels.

Show MeSH

Related in: MedlinePlus

Effect of camel milk on Ho-1 mRNA expression level. Hepa 1c1c7 cells were treated for 6 h with increasing concentrations of fat-free camel milk (0, 25, and 100 μL/mL) or SFN (5 μM), as a positive control. The amount of Ho-1 mRNA was quantified using real-time PCR and normalized to β-actin housekeeping gene. Duplicate reactions were performed for each experiment, and the values represent mean of fold change ± SEM. (n = 4).   +P < 0.05 compared with control (sterile water-treated cells).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3345340&req=5

fig6: Effect of camel milk on Ho-1 mRNA expression level. Hepa 1c1c7 cells were treated for 6 h with increasing concentrations of fat-free camel milk (0, 25, and 100 μL/mL) or SFN (5 μM), as a positive control. The amount of Ho-1 mRNA was quantified using real-time PCR and normalized to β-actin housekeeping gene. Duplicate reactions were performed for each experiment, and the values represent mean of fold change ± SEM. (n = 4).   +P < 0.05 compared with control (sterile water-treated cells).

Mentions: The capacity of camel milk to inhibit TCDD-mediated induction of Cyp1a1 at the activity level more than at the protein levels prompted us to investigate the possible role of Ho-1, a rate-limiting enzyme of heme degradation, in this inhibitory effect. For this purpose, Ho-1 mRNA level was determined in Hepa 1c1c7 cells treated with increasing concentrations of camel milk (0, 25, and 100 μL/mL) or SFN (5 μM), as a positive control, for 6 h. Our results show that camel milk (fat-free) significantly increased the Ho-1 mRNA expression level by the highest concentration tested (100 μL/mL) by approximately 3-fold (Figure 6). Similarly, SFN caused a 9-fold induction in Ho-1 mRNA level. These data may suggest a role for Ho-1 in camel milk-mediated effects.


Camel milk modulates the expression of aryl hydrocarbon receptor-regulated genes, Cyp1a1, Nqo1, and Gsta1, in murine hepatoma Hepa 1c1c7 cells.

Korashy HM, El Gendy MA, Alhaider AA, El-Kadi AO - J. Biomed. Biotechnol. (2012)

Effect of camel milk on Ho-1 mRNA expression level. Hepa 1c1c7 cells were treated for 6 h with increasing concentrations of fat-free camel milk (0, 25, and 100 μL/mL) or SFN (5 μM), as a positive control. The amount of Ho-1 mRNA was quantified using real-time PCR and normalized to β-actin housekeeping gene. Duplicate reactions were performed for each experiment, and the values represent mean of fold change ± SEM. (n = 4).   +P < 0.05 compared with control (sterile water-treated cells).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3345340&req=5

fig6: Effect of camel milk on Ho-1 mRNA expression level. Hepa 1c1c7 cells were treated for 6 h with increasing concentrations of fat-free camel milk (0, 25, and 100 μL/mL) or SFN (5 μM), as a positive control. The amount of Ho-1 mRNA was quantified using real-time PCR and normalized to β-actin housekeeping gene. Duplicate reactions were performed for each experiment, and the values represent mean of fold change ± SEM. (n = 4).   +P < 0.05 compared with control (sterile water-treated cells).
Mentions: The capacity of camel milk to inhibit TCDD-mediated induction of Cyp1a1 at the activity level more than at the protein levels prompted us to investigate the possible role of Ho-1, a rate-limiting enzyme of heme degradation, in this inhibitory effect. For this purpose, Ho-1 mRNA level was determined in Hepa 1c1c7 cells treated with increasing concentrations of camel milk (0, 25, and 100 μL/mL) or SFN (5 μM), as a positive control, for 6 h. Our results show that camel milk (fat-free) significantly increased the Ho-1 mRNA expression level by the highest concentration tested (100 μL/mL) by approximately 3-fold (Figure 6). Similarly, SFN caused a 9-fold induction in Ho-1 mRNA level. These data may suggest a role for Ho-1 in camel milk-mediated effects.

Bottom Line: In addition, camel milk significantly decreased the xenobiotic responsive element (XRE)-dependent luciferase activity, suggesting a transcriptional mechanism is involved.Furthermore, this inhibitory effect of camel milk was associated with a proportional increase in heme oxygenase 1.In conclusion, camel milk modulates the expression of Cyp1a1, Nqo1, and Gsta1 at the transcriptional and posttranscriptional levels.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, 11451 Riyadh, Saudi Arabia.

ABSTRACT
There is a traditional belief in the Middle East that camel milk may aid in prevention and treatment of numerous cases of cancer yet, the exact mechanism was not investigated. Therefore, we examined the ability of camel milk to modulate the expression of a well-known cancer-activating gene, Cytochrome P450 1a1 (Cyp1a1), and cancer-protective genes, NAD(P)H:quinone oxidoreductase 1 (Nqo1) and glutathione S-transferase a1 (Gsta1), in murine hepatoma Hepa 1c1c7 cell line. Our results showed that camel milk significantly inhibited the induction of Cyp1a1 gene expression by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), the most potent Cyp1a1 inducer and known carcinogenic chemical, at mRNA, protein, and activity levels in a concentration-dependent manner. In addition, camel milk significantly decreased the xenobiotic responsive element (XRE)-dependent luciferase activity, suggesting a transcriptional mechanism is involved. Furthermore, this inhibitory effect of camel milk was associated with a proportional increase in heme oxygenase 1. On the other hand, camel milk significantly induced Nqo1 and Gsta1 mRNA expression level in a concentration-dependent fashion. The RNA synthesis inhibitor, actinomycin D, completely blocked the induction of Nqo1 mRNA by camel milk suggesting the requirement of de novo RNA synthesis through a transcriptional mechanism. In conclusion, camel milk modulates the expression of Cyp1a1, Nqo1, and Gsta1 at the transcriptional and posttranscriptional levels.

Show MeSH
Related in: MedlinePlus