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Immunoglobulin E-binding reactivities of natural pollen grain extracts from selected grass species in the Philippines.

Cabauatan CR, Ramos JD - Asia Pac Allergy (2012)

Bottom Line: Pollen grains have been reported to be present in the Philippine atmosphere but studies regarding their allergenicity are limited.Sensitization of the allergic individuals to the pollen extracts was detected with I. cylindrica and O. sativa to be the most frequently recognized with more that 92% reactivity, whereas for C. dactylon and Z. mays, were found to have less than 25% reactivity.Multiple IgE-binding proteins from S. indicus, S. spontaneum and C. barbata that were detected may be responsible for the allergic reactions among Filipino subjects.

View Article: PubMed Central - PubMed

Affiliation: Center for Natural Sciences, School of Arts and Sciences, Saint Mary's University, Bayombong, Nueva Vizcaya 3700, Philippines.

ABSTRACT

Background: Pollen grains have been reported to be present in the Philippine atmosphere but studies regarding their allergenicity are limited.

Objective: The present study aimed to profile the sensitization of allergic individuals to selected grass pollen species and to characterize the pollen proteins that may be responsible for this allergenic response.

Methods: The protein profile of the grass pollen extracts from Cynodon dactylon, Saccharum spontaneum, Sporobulus indicus, Chloris barbata, Oryza sativa, Imperata cylindrica, and Zea mays was analyzed by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis. The specific-IgE profile of the allergic individuals and the allergenic potential of the pollen extracts were evaluated through Enzyme-linked Immunosorbent Assay and IgE immunoblotting.

Results: Sensitization of the allergic individuals to the pollen extracts was detected with I. cylindrica and O. sativa to be the most frequently recognized with more that 92% reactivity, whereas for C. dactylon and Z. mays, were found to have less than 25% reactivity.

Conclusion: Multiple IgE-binding proteins from S. indicus, S. spontaneum and C. barbata that were detected may be responsible for the allergic reactions among Filipino subjects.

No MeSH data available.


Related in: MedlinePlus

IgE Immunoblot of selected sera from allergic patients against Sporobulus indicus (A), Saccharum spontaneum (B) and Chloris barbata (C) pollen extracts.
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Figure 5: IgE Immunoblot of selected sera from allergic patients against Sporobulus indicus (A), Saccharum spontaneum (B) and Chloris barbata (C) pollen extracts.

Mentions: Fig. 5 shows that S. indicus extract have eight bands that could be recognized by seven out of the ten tested sera. These faint but distinct bands ranged from high to low molecular weights (Fig. 5A). High molecular weight proteins recognized are approximately at 37, 50, 75 and 100 kDa while four lower molecular weight proteins were located between approximately 20 to 25 kDa. For S. spontaneum, 8 out of 10 of the sera showed three IgE reactive protein bands. One band is located at the 90 to 100 kDa molecular weight, another at the 55 kDa and one at 37 kDa (Fig. 5B). Three distinct bands were prominent for C. barbata (Fig. 5C). One, which is at 50 kDa was recognized by all of the 10 subjects. A 70 kDa band was recognized by two of the subjects while one band was shown at approximately 100 kDa.


Immunoglobulin E-binding reactivities of natural pollen grain extracts from selected grass species in the Philippines.

Cabauatan CR, Ramos JD - Asia Pac Allergy (2012)

IgE Immunoblot of selected sera from allergic patients against Sporobulus indicus (A), Saccharum spontaneum (B) and Chloris barbata (C) pollen extracts.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3345327&req=5

Figure 5: IgE Immunoblot of selected sera from allergic patients against Sporobulus indicus (A), Saccharum spontaneum (B) and Chloris barbata (C) pollen extracts.
Mentions: Fig. 5 shows that S. indicus extract have eight bands that could be recognized by seven out of the ten tested sera. These faint but distinct bands ranged from high to low molecular weights (Fig. 5A). High molecular weight proteins recognized are approximately at 37, 50, 75 and 100 kDa while four lower molecular weight proteins were located between approximately 20 to 25 kDa. For S. spontaneum, 8 out of 10 of the sera showed three IgE reactive protein bands. One band is located at the 90 to 100 kDa molecular weight, another at the 55 kDa and one at 37 kDa (Fig. 5B). Three distinct bands were prominent for C. barbata (Fig. 5C). One, which is at 50 kDa was recognized by all of the 10 subjects. A 70 kDa band was recognized by two of the subjects while one band was shown at approximately 100 kDa.

Bottom Line: Pollen grains have been reported to be present in the Philippine atmosphere but studies regarding their allergenicity are limited.Sensitization of the allergic individuals to the pollen extracts was detected with I. cylindrica and O. sativa to be the most frequently recognized with more that 92% reactivity, whereas for C. dactylon and Z. mays, were found to have less than 25% reactivity.Multiple IgE-binding proteins from S. indicus, S. spontaneum and C. barbata that were detected may be responsible for the allergic reactions among Filipino subjects.

View Article: PubMed Central - PubMed

Affiliation: Center for Natural Sciences, School of Arts and Sciences, Saint Mary's University, Bayombong, Nueva Vizcaya 3700, Philippines.

ABSTRACT

Background: Pollen grains have been reported to be present in the Philippine atmosphere but studies regarding their allergenicity are limited.

Objective: The present study aimed to profile the sensitization of allergic individuals to selected grass pollen species and to characterize the pollen proteins that may be responsible for this allergenic response.

Methods: The protein profile of the grass pollen extracts from Cynodon dactylon, Saccharum spontaneum, Sporobulus indicus, Chloris barbata, Oryza sativa, Imperata cylindrica, and Zea mays was analyzed by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis. The specific-IgE profile of the allergic individuals and the allergenic potential of the pollen extracts were evaluated through Enzyme-linked Immunosorbent Assay and IgE immunoblotting.

Results: Sensitization of the allergic individuals to the pollen extracts was detected with I. cylindrica and O. sativa to be the most frequently recognized with more that 92% reactivity, whereas for C. dactylon and Z. mays, were found to have less than 25% reactivity.

Conclusion: Multiple IgE-binding proteins from S. indicus, S. spontaneum and C. barbata that were detected may be responsible for the allergic reactions among Filipino subjects.

No MeSH data available.


Related in: MedlinePlus