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Immunoglobulin E-binding reactivities of natural pollen grain extracts from selected grass species in the Philippines.

Cabauatan CR, Ramos JD - Asia Pac Allergy (2012)

Bottom Line: Pollen grains have been reported to be present in the Philippine atmosphere but studies regarding their allergenicity are limited.Sensitization of the allergic individuals to the pollen extracts was detected with I. cylindrica and O. sativa to be the most frequently recognized with more that 92% reactivity, whereas for C. dactylon and Z. mays, were found to have less than 25% reactivity.Multiple IgE-binding proteins from S. indicus, S. spontaneum and C. barbata that were detected may be responsible for the allergic reactions among Filipino subjects.

View Article: PubMed Central - PubMed

Affiliation: Center for Natural Sciences, School of Arts and Sciences, Saint Mary's University, Bayombong, Nueva Vizcaya 3700, Philippines.

ABSTRACT

Background: Pollen grains have been reported to be present in the Philippine atmosphere but studies regarding their allergenicity are limited.

Objective: The present study aimed to profile the sensitization of allergic individuals to selected grass pollen species and to characterize the pollen proteins that may be responsible for this allergenic response.

Methods: The protein profile of the grass pollen extracts from Cynodon dactylon, Saccharum spontaneum, Sporobulus indicus, Chloris barbata, Oryza sativa, Imperata cylindrica, and Zea mays was analyzed by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis. The specific-IgE profile of the allergic individuals and the allergenic potential of the pollen extracts were evaluated through Enzyme-linked Immunosorbent Assay and IgE immunoblotting.

Results: Sensitization of the allergic individuals to the pollen extracts was detected with I. cylindrica and O. sativa to be the most frequently recognized with more that 92% reactivity, whereas for C. dactylon and Z. mays, were found to have less than 25% reactivity.

Conclusion: Multiple IgE-binding proteins from S. indicus, S. spontaneum and C. barbata that were detected may be responsible for the allergic reactions among Filipino subjects.

No MeSH data available.


Related in: MedlinePlus

Sodium dodecyl sulfate polyacrylamide gel electrophoresis profile of the selected grass pollen extracts resolved using a 20% reducing gel and was stained with silver nitrate.
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Figure 2: Sodium dodecyl sulfate polyacrylamide gel electrophoresis profile of the selected grass pollen extracts resolved using a 20% reducing gel and was stained with silver nitrate.

Mentions: The protein concentration of the extracts ranged from 11 to 22 mg/mL. About 22 mg of total protein was extracted for every gram of pollen grains as a starting material. In the SDS-PAGE (Fig. 2), the extracts resolved bands between 250 to 1.7 kDa. Though some of the proteins appear to be degraded, some bands can be seen at specific levels. Bands from Cynodon dactylon ranged from 72 to 4.6 kDa with distinct bands at 70, 28, 17 to 8, and at 4.6 kDa. Prominent bands can be seen at 60, 20 and 12 kDa for S. spontaneum; 26, 17-20 and 12 kDa for S. indicus; and at 95, and 36 kDa for C. barbata. For Oryza Sativa, it has 95 to 1.7 kDa bands which predominated at the 95, 70, 45, 36 to 28, and 20 to 1.7 kDa levels. Imperata cylindrica and Zea mays bands were equally distributed between the 250 to the 4.6 kDa range.


Immunoglobulin E-binding reactivities of natural pollen grain extracts from selected grass species in the Philippines.

Cabauatan CR, Ramos JD - Asia Pac Allergy (2012)

Sodium dodecyl sulfate polyacrylamide gel electrophoresis profile of the selected grass pollen extracts resolved using a 20% reducing gel and was stained with silver nitrate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3345327&req=5

Figure 2: Sodium dodecyl sulfate polyacrylamide gel electrophoresis profile of the selected grass pollen extracts resolved using a 20% reducing gel and was stained with silver nitrate.
Mentions: The protein concentration of the extracts ranged from 11 to 22 mg/mL. About 22 mg of total protein was extracted for every gram of pollen grains as a starting material. In the SDS-PAGE (Fig. 2), the extracts resolved bands between 250 to 1.7 kDa. Though some of the proteins appear to be degraded, some bands can be seen at specific levels. Bands from Cynodon dactylon ranged from 72 to 4.6 kDa with distinct bands at 70, 28, 17 to 8, and at 4.6 kDa. Prominent bands can be seen at 60, 20 and 12 kDa for S. spontaneum; 26, 17-20 and 12 kDa for S. indicus; and at 95, and 36 kDa for C. barbata. For Oryza Sativa, it has 95 to 1.7 kDa bands which predominated at the 95, 70, 45, 36 to 28, and 20 to 1.7 kDa levels. Imperata cylindrica and Zea mays bands were equally distributed between the 250 to the 4.6 kDa range.

Bottom Line: Pollen grains have been reported to be present in the Philippine atmosphere but studies regarding their allergenicity are limited.Sensitization of the allergic individuals to the pollen extracts was detected with I. cylindrica and O. sativa to be the most frequently recognized with more that 92% reactivity, whereas for C. dactylon and Z. mays, were found to have less than 25% reactivity.Multiple IgE-binding proteins from S. indicus, S. spontaneum and C. barbata that were detected may be responsible for the allergic reactions among Filipino subjects.

View Article: PubMed Central - PubMed

Affiliation: Center for Natural Sciences, School of Arts and Sciences, Saint Mary's University, Bayombong, Nueva Vizcaya 3700, Philippines.

ABSTRACT

Background: Pollen grains have been reported to be present in the Philippine atmosphere but studies regarding their allergenicity are limited.

Objective: The present study aimed to profile the sensitization of allergic individuals to selected grass pollen species and to characterize the pollen proteins that may be responsible for this allergenic response.

Methods: The protein profile of the grass pollen extracts from Cynodon dactylon, Saccharum spontaneum, Sporobulus indicus, Chloris barbata, Oryza sativa, Imperata cylindrica, and Zea mays was analyzed by Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis. The specific-IgE profile of the allergic individuals and the allergenic potential of the pollen extracts were evaluated through Enzyme-linked Immunosorbent Assay and IgE immunoblotting.

Results: Sensitization of the allergic individuals to the pollen extracts was detected with I. cylindrica and O. sativa to be the most frequently recognized with more that 92% reactivity, whereas for C. dactylon and Z. mays, were found to have less than 25% reactivity.

Conclusion: Multiple IgE-binding proteins from S. indicus, S. spontaneum and C. barbata that were detected may be responsible for the allergic reactions among Filipino subjects.

No MeSH data available.


Related in: MedlinePlus