Limits...
Antiaging effect of pine pollen in human diploid fibroblasts and in a mouse model induced by D-galactose.

Mao GX, Zheng LD, Cao YB, Chen ZM, Lv YD, Wang YZ, Hu XL, Wang GF, Yan J - Oxid Med Cell Longev (2012)

Bottom Line: The present paper was designed to investigate the effect of pine pollen against aging in human diploid fibroblast 2BS cells and in an accelerated aging model, which was established by subcutaneous injections with D-galactose daily for 8 weeks in C57BL/6J mice.Besides, pine pollen reversed D-galactose-induced aging effects in neural activity and inflammatory cytokine levels, as indicated by improved memory latency time and reduced error rate in step-down test and decreased concentrations of IL-6 and TNF-α in model mice.Similar to the role of AGEs (advanced glycation endproducts) formation inhibitor aminoguanidine (AG), pine pollen inhibited D-galactose-induced increment of AGEs levels thus reversed the aging phenotypes in model mice.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Key Laboratory of Geriatrics, Zhejiang Hospital, Hangzhou, China.

ABSTRACT
The present paper was designed to investigate the effect of pine pollen against aging in human diploid fibroblast 2BS cells and in an accelerated aging model, which was established by subcutaneous injections with D-galactose daily for 8 weeks in C57BL/6J mice. Pine pollen (1 mg/mL and 2 mg/mL) is proved to delay the replicative senescence of 2BS cells as evidenced by enhanced cell proliferation, decreased SA-β-Gal activity, and reversed expression of senescence-associated molecular markers, such as p53, p21(Waf1), p16(INK4a), PTEN, and p27(Kip1) in late PD cells. Besides, pine pollen reversed D-galactose-induced aging effects in neural activity and inflammatory cytokine levels, as indicated by improved memory latency time and reduced error rate in step-down test and decreased concentrations of IL-6 and TNF-α in model mice. Similar to the role of AGEs (advanced glycation endproducts) formation inhibitor aminoguanidine (AG), pine pollen inhibited D-galactose-induced increment of AGEs levels thus reversed the aging phenotypes in model mice. Furthermore, the declined antioxidant activity was obviously reversed upon pine pollen treatment, which may account for its inhibitory effect on nonenzymatic glycation (NEG) in vivo. Our finding presents pine pollen as an attractive agent with potential to retard aging and attenuate age-related diseases in humans.

Show MeSH

Related in: MedlinePlus

SA-β-gal staining of 2BS cells grown from PD30 in DMEM supplemented with 1 mg/mL or 2 mg/mL pine pollen (PP). Cells of none-confluent state were washed with PBS, fixed with 3% formaldehyde, and stained in staining solution containing 1 mg/mL 5-bromo-4-chloro-3-indolyl-β-D-galactoside for 16 h. 2BS cells at PD30 were set as young control. Cells were microphotographed at a magnification of 10 × 10. *P < 0.01 versus 30PD control group; #P < 0.01 versus 55PD control group.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3345248&req=5

fig2: SA-β-gal staining of 2BS cells grown from PD30 in DMEM supplemented with 1 mg/mL or 2 mg/mL pine pollen (PP). Cells of none-confluent state were washed with PBS, fixed with 3% formaldehyde, and stained in staining solution containing 1 mg/mL 5-bromo-4-chloro-3-indolyl-β-D-galactoside for 16 h. 2BS cells at PD30 were set as young control. Cells were microphotographed at a magnification of 10 × 10. *P < 0.01 versus 30PD control group; #P < 0.01 versus 55PD control group.

Mentions: As expected, only sporadic SA-β-gal positive cells were observed in young control cells. As shown in Figure 2, the SA-β-gal positive rates of 1 mg/mL and 2 mg/mL pine pollen treatment PD55 cells were 27.2 ± 4.3% and 25.3 ± 4.9%, respectively, which were much lower than that of PD55 control cells (81.7 ± 7.1%). These results indicate indirectly that the pine pollen delayed the population senescence of 2BS cells.


Antiaging effect of pine pollen in human diploid fibroblasts and in a mouse model induced by D-galactose.

Mao GX, Zheng LD, Cao YB, Chen ZM, Lv YD, Wang YZ, Hu XL, Wang GF, Yan J - Oxid Med Cell Longev (2012)

SA-β-gal staining of 2BS cells grown from PD30 in DMEM supplemented with 1 mg/mL or 2 mg/mL pine pollen (PP). Cells of none-confluent state were washed with PBS, fixed with 3% formaldehyde, and stained in staining solution containing 1 mg/mL 5-bromo-4-chloro-3-indolyl-β-D-galactoside for 16 h. 2BS cells at PD30 were set as young control. Cells were microphotographed at a magnification of 10 × 10. *P < 0.01 versus 30PD control group; #P < 0.01 versus 55PD control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3345248&req=5

fig2: SA-β-gal staining of 2BS cells grown from PD30 in DMEM supplemented with 1 mg/mL or 2 mg/mL pine pollen (PP). Cells of none-confluent state were washed with PBS, fixed with 3% formaldehyde, and stained in staining solution containing 1 mg/mL 5-bromo-4-chloro-3-indolyl-β-D-galactoside for 16 h. 2BS cells at PD30 were set as young control. Cells were microphotographed at a magnification of 10 × 10. *P < 0.01 versus 30PD control group; #P < 0.01 versus 55PD control group.
Mentions: As expected, only sporadic SA-β-gal positive cells were observed in young control cells. As shown in Figure 2, the SA-β-gal positive rates of 1 mg/mL and 2 mg/mL pine pollen treatment PD55 cells were 27.2 ± 4.3% and 25.3 ± 4.9%, respectively, which were much lower than that of PD55 control cells (81.7 ± 7.1%). These results indicate indirectly that the pine pollen delayed the population senescence of 2BS cells.

Bottom Line: The present paper was designed to investigate the effect of pine pollen against aging in human diploid fibroblast 2BS cells and in an accelerated aging model, which was established by subcutaneous injections with D-galactose daily for 8 weeks in C57BL/6J mice.Besides, pine pollen reversed D-galactose-induced aging effects in neural activity and inflammatory cytokine levels, as indicated by improved memory latency time and reduced error rate in step-down test and decreased concentrations of IL-6 and TNF-α in model mice.Similar to the role of AGEs (advanced glycation endproducts) formation inhibitor aminoguanidine (AG), pine pollen inhibited D-galactose-induced increment of AGEs levels thus reversed the aging phenotypes in model mice.

View Article: PubMed Central - PubMed

Affiliation: Zhejiang Provincial Key Laboratory of Geriatrics, Zhejiang Hospital, Hangzhou, China.

ABSTRACT
The present paper was designed to investigate the effect of pine pollen against aging in human diploid fibroblast 2BS cells and in an accelerated aging model, which was established by subcutaneous injections with D-galactose daily for 8 weeks in C57BL/6J mice. Pine pollen (1 mg/mL and 2 mg/mL) is proved to delay the replicative senescence of 2BS cells as evidenced by enhanced cell proliferation, decreased SA-β-Gal activity, and reversed expression of senescence-associated molecular markers, such as p53, p21(Waf1), p16(INK4a), PTEN, and p27(Kip1) in late PD cells. Besides, pine pollen reversed D-galactose-induced aging effects in neural activity and inflammatory cytokine levels, as indicated by improved memory latency time and reduced error rate in step-down test and decreased concentrations of IL-6 and TNF-α in model mice. Similar to the role of AGEs (advanced glycation endproducts) formation inhibitor aminoguanidine (AG), pine pollen inhibited D-galactose-induced increment of AGEs levels thus reversed the aging phenotypes in model mice. Furthermore, the declined antioxidant activity was obviously reversed upon pine pollen treatment, which may account for its inhibitory effect on nonenzymatic glycation (NEG) in vivo. Our finding presents pine pollen as an attractive agent with potential to retard aging and attenuate age-related diseases in humans.

Show MeSH
Related in: MedlinePlus