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Changes in surface-charge density of blood cells after sudden unexpected death.

Kotyńska J, Petelska AD, Szeremeta M, Niemcunowicz-Janica A, Figaszewski ZA - J. Membr. Biol. (2012)

Bottom Line: Values of parameters characterizing the membrane--that is, the total surface concentrations of both acidic and basic groups and their association constants with solution ions--were calculated on the basis of a four-equilibria mathematical model.The model was validated by comparison of these values to experimental data.We established that examined electric properties of the cell membranes are affected by sudden unexpected death.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemistry, University of Bialystok, Al. J. Pilsudskiego 11/4, 15-443 Bialystok, Poland.

ABSTRACT
The objective of the investigation was evaluation of postmortem changes of electric charge of human erythrocyte and thrombocyte membranes after sudden unexpected death. The surface charge density values were determined on the basis of the electrophoretic mobility measurements of the cells carried out at various pHs of electrolyte solution. The interactions between both erythrocyte and thrombocyte membranes and electrolyte ions were studied. Values of parameters characterizing the membrane--that is, the total surface concentrations of both acidic and basic groups and their association constants with solution ions--were calculated on the basis of a four-equilibria mathematical model. The model was validated by comparison of these values to experimental data. We established that examined electric properties of the cell membranes are affected by sudden unexpected death. Postmortem processes occurring in the cell membranes can lead to disorders of existing equilibria, which in turn result in changes in values of all the above-mentioned parameters.

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pH dependence of surface charge density of erythrocytes. Circles control, squares sudden unexpected death. Experimental values are indicated by points and the theoretical values by the curve
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Fig1: pH dependence of surface charge density of erythrocytes. Circles control, squares sudden unexpected death. Experimental values are indicated by points and the theoretical values by the curve

Mentions: Both experimental and theoretical surface charge density values of the cell membranes as a function of pH are presented in Figs. 1 and 2. The former are indicated by points, and the latter are indicated by curves.Fig. 1


Changes in surface-charge density of blood cells after sudden unexpected death.

Kotyńska J, Petelska AD, Szeremeta M, Niemcunowicz-Janica A, Figaszewski ZA - J. Membr. Biol. (2012)

pH dependence of surface charge density of erythrocytes. Circles control, squares sudden unexpected death. Experimental values are indicated by points and the theoretical values by the curve
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3345184&req=5

Fig1: pH dependence of surface charge density of erythrocytes. Circles control, squares sudden unexpected death. Experimental values are indicated by points and the theoretical values by the curve
Mentions: Both experimental and theoretical surface charge density values of the cell membranes as a function of pH are presented in Figs. 1 and 2. The former are indicated by points, and the latter are indicated by curves.Fig. 1

Bottom Line: Values of parameters characterizing the membrane--that is, the total surface concentrations of both acidic and basic groups and their association constants with solution ions--were calculated on the basis of a four-equilibria mathematical model.The model was validated by comparison of these values to experimental data.We established that examined electric properties of the cell membranes are affected by sudden unexpected death.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemistry, University of Bialystok, Al. J. Pilsudskiego 11/4, 15-443 Bialystok, Poland.

ABSTRACT
The objective of the investigation was evaluation of postmortem changes of electric charge of human erythrocyte and thrombocyte membranes after sudden unexpected death. The surface charge density values were determined on the basis of the electrophoretic mobility measurements of the cells carried out at various pHs of electrolyte solution. The interactions between both erythrocyte and thrombocyte membranes and electrolyte ions were studied. Values of parameters characterizing the membrane--that is, the total surface concentrations of both acidic and basic groups and their association constants with solution ions--were calculated on the basis of a four-equilibria mathematical model. The model was validated by comparison of these values to experimental data. We established that examined electric properties of the cell membranes are affected by sudden unexpected death. Postmortem processes occurring in the cell membranes can lead to disorders of existing equilibria, which in turn result in changes in values of all the above-mentioned parameters.

Show MeSH
Related in: MedlinePlus