Limits...
Protective effect of blackcurrant on liver cell membrane of rats intoxicated with ethanol.

Szachowicz-Petelska B, Dobrzyńska I, Skrzydlewska E, Figaszewski Z - J. Membr. Biol. (2012)

Bottom Line: Ethanol increased phospholipid levels and altered the level of integral proteins as determined by decreased phenylalanine, cysteine, and lysine.Ethanol significantly enhanced changes in the surface charge density of the liver cell membranes.It is possible that the beneficial effect of blackcurrant is connected with its abilities to scavenge free radicals and to chelate metal ions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemistry, University in Białystok, Al. Piłsudskiego 11/4, 15-443 Białystok, Poland.

ABSTRACT
Chronic ethanol intoxication oxidative stress participates in the development of many diseases. Nutrition and the interaction of food nutrients with ethanol metabolism may modulate alcohol toxicity. One such compound is blackcurrant, which also has antioxidant abilities. We investigated the effect of blackcurrant as an antioxidant on the composition and electrical charge of liver cell membranes in ethanol-intoxicated rats. Qualitative and quantitative phospholipid composition and the presence of integral membrane proteins were determined by high-performance liquid chromatography. Electrophoresis was used to determine the surface charge density of the rat liver cell membranes. Ethanol intoxication is characterized by changes in cell metabolism that alter the structure and function of cell membrane components. Ethanol increased phospholipid levels and altered the level of integral proteins as determined by decreased phenylalanine, cysteine, and lysine. Ethanol significantly enhanced changes in the surface charge density of the liver cell membranes. Administration of blackcurrant to rats intoxicated with ethanol significantly protected lipids and proteins against oxidative modifications. It is possible that the beneficial effect of blackcurrant is connected with its abilities to scavenge free radicals and to chelate metal ions.

Show MeSH

Related in: MedlinePlus

Typical separation of peptides from liver integral membrane proteins (UV detected at 220 nm)
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3345183&req=5

Fig1: Typical separation of peptides from liver integral membrane proteins (UV detected at 220 nm)

Mentions: After hydrolysis, the peptides were separated by HPLC on a LichroCART RP-18 column 100A (5 μm, 250 × 4.0 mm) equilibrated with solvent A (0.1 % trifluoroacetic acid (TFA) in H2O) and eluted with a linear gradient to 20 % solvent B (0.1 % TFA in acetonitrile) during the first 8 min, to 70 % solvent B during the next 20 min, and to 100 % solvent B during the final 4 min at 220 nm. The flow rate was 1 ml/min (Skrzydlewska et al. 1998). The Merck HPLC system was equipped with a pump, a UV detector, an analog interface module D-6000 A, and System Manager software. A typical separation of the peptide mixture containing liver integral membrane proteins is provided in Fig. 1.Fig. 1


Protective effect of blackcurrant on liver cell membrane of rats intoxicated with ethanol.

Szachowicz-Petelska B, Dobrzyńska I, Skrzydlewska E, Figaszewski Z - J. Membr. Biol. (2012)

Typical separation of peptides from liver integral membrane proteins (UV detected at 220 nm)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3345183&req=5

Fig1: Typical separation of peptides from liver integral membrane proteins (UV detected at 220 nm)
Mentions: After hydrolysis, the peptides were separated by HPLC on a LichroCART RP-18 column 100A (5 μm, 250 × 4.0 mm) equilibrated with solvent A (0.1 % trifluoroacetic acid (TFA) in H2O) and eluted with a linear gradient to 20 % solvent B (0.1 % TFA in acetonitrile) during the first 8 min, to 70 % solvent B during the next 20 min, and to 100 % solvent B during the final 4 min at 220 nm. The flow rate was 1 ml/min (Skrzydlewska et al. 1998). The Merck HPLC system was equipped with a pump, a UV detector, an analog interface module D-6000 A, and System Manager software. A typical separation of the peptide mixture containing liver integral membrane proteins is provided in Fig. 1.Fig. 1

Bottom Line: Ethanol increased phospholipid levels and altered the level of integral proteins as determined by decreased phenylalanine, cysteine, and lysine.Ethanol significantly enhanced changes in the surface charge density of the liver cell membranes.It is possible that the beneficial effect of blackcurrant is connected with its abilities to scavenge free radicals and to chelate metal ions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemistry, University in Białystok, Al. Piłsudskiego 11/4, 15-443 Białystok, Poland.

ABSTRACT
Chronic ethanol intoxication oxidative stress participates in the development of many diseases. Nutrition and the interaction of food nutrients with ethanol metabolism may modulate alcohol toxicity. One such compound is blackcurrant, which also has antioxidant abilities. We investigated the effect of blackcurrant as an antioxidant on the composition and electrical charge of liver cell membranes in ethanol-intoxicated rats. Qualitative and quantitative phospholipid composition and the presence of integral membrane proteins were determined by high-performance liquid chromatography. Electrophoresis was used to determine the surface charge density of the rat liver cell membranes. Ethanol intoxication is characterized by changes in cell metabolism that alter the structure and function of cell membrane components. Ethanol increased phospholipid levels and altered the level of integral proteins as determined by decreased phenylalanine, cysteine, and lysine. Ethanol significantly enhanced changes in the surface charge density of the liver cell membranes. Administration of blackcurrant to rats intoxicated with ethanol significantly protected lipids and proteins against oxidative modifications. It is possible that the beneficial effect of blackcurrant is connected with its abilities to scavenge free radicals and to chelate metal ions.

Show MeSH
Related in: MedlinePlus