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Harmful effects of carbamazepine on the postnatal development of the rat ventral prostate.

Oliva SU, Scarano WR, Okada FK, Miraglia SM - Reprod. Biol. Endocrinol. (2012)

Bottom Line: At the end of each treatment period, ventral prostates were removed and histologically processed.The prostate sections were submitted to the histopathological, morphological and stereological analyses using image analysis system.The results suggest a direct effect of the CBZ on rat ventral prostate, evidenced by increase of mast cell and macrophage populations during pre-puberty and puberty causing a ventral prostate accentuated damage in the adult phase.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Morphology and Genetics, Developmental Biology Laboratory, Federal University of São Paulo, Brazil.

ABSTRACT

Background: Carbamazepine (CBZ) is a first-line antiepileptic drug (AED), although it is also used for the treatments of psychiatric disorders and neuropathic pain. The CBZ utilization has been associated with male reproductive damage, including hormonal alterations, sexual dysfunction and reduction of sperm quality. The wide and long-term use of the CBZ is a common schedule in children and adolescents and alters the testosterone level in adult rats and humans. The objective of this work was to evaluate the CBZ side effects on the ventral prostate of rats from pre-puberty to sexual maturation, since the prostate is an androgen-dependent organ.

Methods: Twenty three day-old male albino Wistar rats received CBZ diluted in propylene glycol (20 mg/Kg/i.p via). The treatment lasted 20, 40 and 70 days, according to the different stages of the rat sexual maturation. At the end of each treatment period, ventral prostates were removed and histologically processed. The prostate sections were submitted to the histopathological, morphological and stereological analyses using image analysis system.

Results: Reductions of the glandular epithelium, glandular lumen and fibromuscular stroma volume of the ventral prostate were observed in adult rats treated with CBZ since the weaning. Triggering and degranulation of mast cells were observed in the fibromuscular stroma of prepubertal and pubertal CBZ treated rats.

Conclusions: The results suggest a direct effect of the CBZ on rat ventral prostate, evidenced by increase of mast cell and macrophage populations during pre-puberty and puberty causing a ventral prostate accentuated damage in the adult phase.

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Photomicrographs of ventral prostate sections of control and CBZ- treated rats at different ages submitted to AR IHC. Brown stain signifies AR positive reactivity. Observe immuno-stainable AR located in the nuclei of cells in the prostate epithelium (arrows); AR immuno-localization showed decrease in staining intensity in 93- day old CBZ-treated rats when compared with their control rats. Ep: glandular epithelium; Lu: glandular lumen. Bars: 15 μm.
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Figure 8: Photomicrographs of ventral prostate sections of control and CBZ- treated rats at different ages submitted to AR IHC. Brown stain signifies AR positive reactivity. Observe immuno-stainable AR located in the nuclei of cells in the prostate epithelium (arrows); AR immuno-localization showed decrease in staining intensity in 93- day old CBZ-treated rats when compared with their control rats. Ep: glandular epithelium; Lu: glandular lumen. Bars: 15 μm.

Mentions: The ventral prostate AR immunoreactivity presented the same pattern of staining in the following control and treated groups: C43 and CBZ43; C63 and CBZ63 (Figures 8A-D). However, AR immunoreactivity was diminished in the CBZ93 group in relation to C93 control group (Figures 8E, F). The optical density of nuclear reactivity confirmed the qualitative analysis and showed decrease in the AR reactivity in the CBZ93 group when compared to the C93 control group (Figure 9).


Harmful effects of carbamazepine on the postnatal development of the rat ventral prostate.

Oliva SU, Scarano WR, Okada FK, Miraglia SM - Reprod. Biol. Endocrinol. (2012)

Photomicrographs of ventral prostate sections of control and CBZ- treated rats at different ages submitted to AR IHC. Brown stain signifies AR positive reactivity. Observe immuno-stainable AR located in the nuclei of cells in the prostate epithelium (arrows); AR immuno-localization showed decrease in staining intensity in 93- day old CBZ-treated rats when compared with their control rats. Ep: glandular epithelium; Lu: glandular lumen. Bars: 15 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3344685&req=5

Figure 8: Photomicrographs of ventral prostate sections of control and CBZ- treated rats at different ages submitted to AR IHC. Brown stain signifies AR positive reactivity. Observe immuno-stainable AR located in the nuclei of cells in the prostate epithelium (arrows); AR immuno-localization showed decrease in staining intensity in 93- day old CBZ-treated rats when compared with their control rats. Ep: glandular epithelium; Lu: glandular lumen. Bars: 15 μm.
Mentions: The ventral prostate AR immunoreactivity presented the same pattern of staining in the following control and treated groups: C43 and CBZ43; C63 and CBZ63 (Figures 8A-D). However, AR immunoreactivity was diminished in the CBZ93 group in relation to C93 control group (Figures 8E, F). The optical density of nuclear reactivity confirmed the qualitative analysis and showed decrease in the AR reactivity in the CBZ93 group when compared to the C93 control group (Figure 9).

Bottom Line: At the end of each treatment period, ventral prostates were removed and histologically processed.The prostate sections were submitted to the histopathological, morphological and stereological analyses using image analysis system.The results suggest a direct effect of the CBZ on rat ventral prostate, evidenced by increase of mast cell and macrophage populations during pre-puberty and puberty causing a ventral prostate accentuated damage in the adult phase.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Morphology and Genetics, Developmental Biology Laboratory, Federal University of São Paulo, Brazil.

ABSTRACT

Background: Carbamazepine (CBZ) is a first-line antiepileptic drug (AED), although it is also used for the treatments of psychiatric disorders and neuropathic pain. The CBZ utilization has been associated with male reproductive damage, including hormonal alterations, sexual dysfunction and reduction of sperm quality. The wide and long-term use of the CBZ is a common schedule in children and adolescents and alters the testosterone level in adult rats and humans. The objective of this work was to evaluate the CBZ side effects on the ventral prostate of rats from pre-puberty to sexual maturation, since the prostate is an androgen-dependent organ.

Methods: Twenty three day-old male albino Wistar rats received CBZ diluted in propylene glycol (20 mg/Kg/i.p via). The treatment lasted 20, 40 and 70 days, according to the different stages of the rat sexual maturation. At the end of each treatment period, ventral prostates were removed and histologically processed. The prostate sections were submitted to the histopathological, morphological and stereological analyses using image analysis system.

Results: Reductions of the glandular epithelium, glandular lumen and fibromuscular stroma volume of the ventral prostate were observed in adult rats treated with CBZ since the weaning. Triggering and degranulation of mast cells were observed in the fibromuscular stroma of prepubertal and pubertal CBZ treated rats.

Conclusions: The results suggest a direct effect of the CBZ on rat ventral prostate, evidenced by increase of mast cell and macrophage populations during pre-puberty and puberty causing a ventral prostate accentuated damage in the adult phase.

Show MeSH
Related in: MedlinePlus