Limits...
Anti-Inflammatory Activities of Cinnamomum cassia Constituents In Vitro and In Vivo.

Liao JC, Deng JS, Chiu CS, Hou WC, Huang SS, Shie PH, Huang GJ - Evid Based Complement Alternat Med (2012)

Bottom Line: Western blotting revealed that cinnamic aldehyde blocked protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear transcription factor kappa B (NF-κB), and IκBα, significantly.Cinnamic aldehyde decreased the NO, TNF-α, and PGE(2) levels on the serum level after Carr injection.These findings demonstrated that cinnamic aldehyde has excellent anti-inflammatory activities and thus has great potential to be used as a source for natural health products.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan.

ABSTRACT
We have investigated the anti-inflammatory effects of Cinnamomum cassia constituents (cinnamic aldehyde, cinnamic alcohol, cinnamic acid, and coumarin) using lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) and carrageenan (Carr)-induced mouse paw edema model. When RAW264.7 macrophages were treated with cinnamic aldehyde together with LPS, a significant concentration-dependent inhibition of nitric oxide (NO), tumor necrosis factor (TNF-α), and prostaglandin E2 (PGE(2)) levels productions were detected. Western blotting revealed that cinnamic aldehyde blocked protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear transcription factor kappa B (NF-κB), and IκBα, significantly. In the anti-inflammatory test, cinnamic aldehyde decreased the paw edema after Carr administration, and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the paw tissue. We also demonstrated cinnamic aldehyde attenuated the malondialdehyde (MDA) level and myeloperoxidase (MPO) activity in the edema paw after Carr injection. Cinnamic aldehyde decreased the NO, TNF-α, and PGE(2) levels on the serum level after Carr injection. Western blotting revealed that cinnamic aldehyde decreased Carr-induced iNOS, COX-2, and NF-κB expressions in the edema paw. These findings demonstrated that cinnamic aldehyde has excellent anti-inflammatory activities and thus has great potential to be used as a source for natural health products.

No MeSH data available.


Related in: MedlinePlus

Chemical structure of Cinnamomum cassia constituents (cinnamic aldehyde, cinnamic alcohol, cinnamic acid, and coumarin) (a) and cytotoxic effects of Cinnamomum cassia constituents in RAW264.7 cells (b). Cells were incubated for 24 h with 100 ng/mL of LPS in the absence or presence of samples (0, 6.25, 12.5, 25, and 50 μM). Samples were added 1 h before incubation with LPS (lipopolysaccharide). Cell viability assay was performed using MTT assay. The data were presented as mean ± S.D. for three different experiments performed in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3318905&req=5

fig1: Chemical structure of Cinnamomum cassia constituents (cinnamic aldehyde, cinnamic alcohol, cinnamic acid, and coumarin) (a) and cytotoxic effects of Cinnamomum cassia constituents in RAW264.7 cells (b). Cells were incubated for 24 h with 100 ng/mL of LPS in the absence or presence of samples (0, 6.25, 12.5, 25, and 50 μM). Samples were added 1 h before incubation with LPS (lipopolysaccharide). Cell viability assay was performed using MTT assay. The data were presented as mean ± S.D. for three different experiments performed in triplicate.

Mentions: LPS (endotoxin from Escherichia coli, serotype 0127:B8), Carr, indomethacin, cinnamic aldehyde (≥98%), cinnamic alcohol (≥98%), cinnamic acid (≥99%), coumarin (≥99%) (Figure 1(a)) and other chemicals were purchased from Sigma Chemical Co. (St. Louis, USA). TNF-α and PGE2 were purchased from Biosource International Inc. (Camarillo, CA, USA). Anti-iNOS, anti-COX-2, anti-NF-κB, anti-IκBα, and anti-β-actin antibody (Santa Cruz, USA) and a protein assay kit (Bio-Rad Laboratories Ltd., Watford, Herts, UK) were obtained as indicated. Poly-(vinylidene fluoride) membrane (Immobilon-P) was obtained from Millipore Corp. (Bedford, MA, USA).


Anti-Inflammatory Activities of Cinnamomum cassia Constituents In Vitro and In Vivo.

Liao JC, Deng JS, Chiu CS, Hou WC, Huang SS, Shie PH, Huang GJ - Evid Based Complement Alternat Med (2012)

Chemical structure of Cinnamomum cassia constituents (cinnamic aldehyde, cinnamic alcohol, cinnamic acid, and coumarin) (a) and cytotoxic effects of Cinnamomum cassia constituents in RAW264.7 cells (b). Cells were incubated for 24 h with 100 ng/mL of LPS in the absence or presence of samples (0, 6.25, 12.5, 25, and 50 μM). Samples were added 1 h before incubation with LPS (lipopolysaccharide). Cell viability assay was performed using MTT assay. The data were presented as mean ± S.D. for three different experiments performed in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3318905&req=5

fig1: Chemical structure of Cinnamomum cassia constituents (cinnamic aldehyde, cinnamic alcohol, cinnamic acid, and coumarin) (a) and cytotoxic effects of Cinnamomum cassia constituents in RAW264.7 cells (b). Cells were incubated for 24 h with 100 ng/mL of LPS in the absence or presence of samples (0, 6.25, 12.5, 25, and 50 μM). Samples were added 1 h before incubation with LPS (lipopolysaccharide). Cell viability assay was performed using MTT assay. The data were presented as mean ± S.D. for three different experiments performed in triplicate.
Mentions: LPS (endotoxin from Escherichia coli, serotype 0127:B8), Carr, indomethacin, cinnamic aldehyde (≥98%), cinnamic alcohol (≥98%), cinnamic acid (≥99%), coumarin (≥99%) (Figure 1(a)) and other chemicals were purchased from Sigma Chemical Co. (St. Louis, USA). TNF-α and PGE2 were purchased from Biosource International Inc. (Camarillo, CA, USA). Anti-iNOS, anti-COX-2, anti-NF-κB, anti-IκBα, and anti-β-actin antibody (Santa Cruz, USA) and a protein assay kit (Bio-Rad Laboratories Ltd., Watford, Herts, UK) were obtained as indicated. Poly-(vinylidene fluoride) membrane (Immobilon-P) was obtained from Millipore Corp. (Bedford, MA, USA).

Bottom Line: Western blotting revealed that cinnamic aldehyde blocked protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear transcription factor kappa B (NF-κB), and IκBα, significantly.Cinnamic aldehyde decreased the NO, TNF-α, and PGE(2) levels on the serum level after Carr injection.These findings demonstrated that cinnamic aldehyde has excellent anti-inflammatory activities and thus has great potential to be used as a source for natural health products.

View Article: PubMed Central - PubMed

Affiliation: School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan.

ABSTRACT
We have investigated the anti-inflammatory effects of Cinnamomum cassia constituents (cinnamic aldehyde, cinnamic alcohol, cinnamic acid, and coumarin) using lipopolysaccharide (LPS)-stimulated mouse macrophage (RAW264.7) and carrageenan (Carr)-induced mouse paw edema model. When RAW264.7 macrophages were treated with cinnamic aldehyde together with LPS, a significant concentration-dependent inhibition of nitric oxide (NO), tumor necrosis factor (TNF-α), and prostaglandin E2 (PGE(2)) levels productions were detected. Western blotting revealed that cinnamic aldehyde blocked protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear transcription factor kappa B (NF-κB), and IκBα, significantly. In the anti-inflammatory test, cinnamic aldehyde decreased the paw edema after Carr administration, and increased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the paw tissue. We also demonstrated cinnamic aldehyde attenuated the malondialdehyde (MDA) level and myeloperoxidase (MPO) activity in the edema paw after Carr injection. Cinnamic aldehyde decreased the NO, TNF-α, and PGE(2) levels on the serum level after Carr injection. Western blotting revealed that cinnamic aldehyde decreased Carr-induced iNOS, COX-2, and NF-κB expressions in the edema paw. These findings demonstrated that cinnamic aldehyde has excellent anti-inflammatory activities and thus has great potential to be used as a source for natural health products.

No MeSH data available.


Related in: MedlinePlus