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In vitro sensitivity of leukemia cells to propranolol.

Hajighasemi F, Mirshafiey A - J Clin Med Res (2009)

Bottom Line: Our results demonstrated that leukemic cell lines used in this study were sensitive to propranolol at ≥ 0.2 mM concentration of the drug.These results suggest that propranolol may have potential implication in chemoprevention of lymphoproliferative disorders along with its chronic long-term usage in cardiac problems.Propranolol; Leukemia; Cell lines; Sensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran.

ABSTRACT

Background: Propranolol, as a beta-adrenergic blocker is used for treatment of a large number of cardiovascular diseases such as hypertension and arrhythmias. The inhibitory effects of propranolol on tumor cells growth and also its cytotoxicity on cancerous cells have been revealed by several studies. In this study the sensitivity of a number of human leukemic cell lines to propranolol was evaluated in vitro.

Methods: Two human leukemic T cells (Molt-4 and Jurkat) and a monocyte (U937) cell line were used in this study. The cells were cultured in complete RPMI medium and then incubated with different concentrations of propranolol (0.0004 -0.4 mM) in the presence or absence of phytoheamagglutinin (20 μg/ml) for 12, 24 and 48 hours. The cytotoxic effect of the drug was then assessed by trypan blue dye exclusion and also 3-(4,5-dimethyl thiazol-2,5-diphenyltetrazoliumbromide) (MTT) reduction methods.

Results: Propranolol induced a significant dose dependent cytotoxic effect at ≥ 0.2 mM concentration on all three human cell lines (Molt-4, Jurkat and U937) used in this study, after 12 hours incubation onwards, compared to untreated control cells.

Conclusions: Our results demonstrated that leukemic cell lines used in this study were sensitive to propranolol at ≥ 0.2 mM concentration of the drug. These results suggest that propranolol may have potential implication in chemoprevention of lymphoproliferative disorders along with its chronic long-term usage in cardiac problems.

Keywords: Propranolol; Leukemia; Cell lines; Sensitivity.

No MeSH data available.


Related in: MedlinePlus

Effect of propranolol on proliferative responses of human leukemic Jurkat T-cell line. The Jurkat cells were treated with different concentrations of propranolol (0.0004 - 0.4 mM) for 12, 24 and 48 hours. The results are presented as % of viability demonstrated by trypan blue dye exclusion (TB) test (A) and cell number/control demonstrated by MTT assay (B). Data are mean ± SD of triplicate cultures. n = 3; P < 0.05 was considered significant.
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Figure 2: Effect of propranolol on proliferative responses of human leukemic Jurkat T-cell line. The Jurkat cells were treated with different concentrations of propranolol (0.0004 - 0.4 mM) for 12, 24 and 48 hours. The results are presented as % of viability demonstrated by trypan blue dye exclusion (TB) test (A) and cell number/control demonstrated by MTT assay (B). Data are mean ± SD of triplicate cultures. n = 3; P < 0.05 was considered significant.

Mentions: According to Figure 2(A and B), propranolol significantly inhibited proliferation of Jurkat cells at ≥ 0.2 mM propranolol concentration after 12 hours incubation compared with untreated control cells (P < 0.05 ).


In vitro sensitivity of leukemia cells to propranolol.

Hajighasemi F, Mirshafiey A - J Clin Med Res (2009)

Effect of propranolol on proliferative responses of human leukemic Jurkat T-cell line. The Jurkat cells were treated with different concentrations of propranolol (0.0004 - 0.4 mM) for 12, 24 and 48 hours. The results are presented as % of viability demonstrated by trypan blue dye exclusion (TB) test (A) and cell number/control demonstrated by MTT assay (B). Data are mean ± SD of triplicate cultures. n = 3; P < 0.05 was considered significant.
© Copyright Policy - open access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3318877&req=5

Figure 2: Effect of propranolol on proliferative responses of human leukemic Jurkat T-cell line. The Jurkat cells were treated with different concentrations of propranolol (0.0004 - 0.4 mM) for 12, 24 and 48 hours. The results are presented as % of viability demonstrated by trypan blue dye exclusion (TB) test (A) and cell number/control demonstrated by MTT assay (B). Data are mean ± SD of triplicate cultures. n = 3; P < 0.05 was considered significant.
Mentions: According to Figure 2(A and B), propranolol significantly inhibited proliferation of Jurkat cells at ≥ 0.2 mM propranolol concentration after 12 hours incubation compared with untreated control cells (P < 0.05 ).

Bottom Line: Our results demonstrated that leukemic cell lines used in this study were sensitive to propranolol at ≥ 0.2 mM concentration of the drug.These results suggest that propranolol may have potential implication in chemoprevention of lymphoproliferative disorders along with its chronic long-term usage in cardiac problems.Propranolol; Leukemia; Cell lines; Sensitivity.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Faculty of Medicine, Shahed University, Tehran, Iran.

ABSTRACT

Background: Propranolol, as a beta-adrenergic blocker is used for treatment of a large number of cardiovascular diseases such as hypertension and arrhythmias. The inhibitory effects of propranolol on tumor cells growth and also its cytotoxicity on cancerous cells have been revealed by several studies. In this study the sensitivity of a number of human leukemic cell lines to propranolol was evaluated in vitro.

Methods: Two human leukemic T cells (Molt-4 and Jurkat) and a monocyte (U937) cell line were used in this study. The cells were cultured in complete RPMI medium and then incubated with different concentrations of propranolol (0.0004 -0.4 mM) in the presence or absence of phytoheamagglutinin (20 μg/ml) for 12, 24 and 48 hours. The cytotoxic effect of the drug was then assessed by trypan blue dye exclusion and also 3-(4,5-dimethyl thiazol-2,5-diphenyltetrazoliumbromide) (MTT) reduction methods.

Results: Propranolol induced a significant dose dependent cytotoxic effect at ≥ 0.2 mM concentration on all three human cell lines (Molt-4, Jurkat and U937) used in this study, after 12 hours incubation onwards, compared to untreated control cells.

Conclusions: Our results demonstrated that leukemic cell lines used in this study were sensitive to propranolol at ≥ 0.2 mM concentration of the drug. These results suggest that propranolol may have potential implication in chemoprevention of lymphoproliferative disorders along with its chronic long-term usage in cardiac problems.

Keywords: Propranolol; Leukemia; Cell lines; Sensitivity.

No MeSH data available.


Related in: MedlinePlus