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Anti-proliferation Effect of Polypeptide Extracted from Scorpion Venom on Human Prostate Cancer Cells in vitro.

Zhang YY, Wu LC, Wang ZP, Wang ZX, Jia Q, Jiang GS, Zhang WD - J Clin Med Res (2009)

Bottom Line: PESV treatment on these cells resulted in a significantly dose-dependent growth inhibition with a G1 phase arrest at 40μg/mL after 48h treatment.PESV treatment strongly induced expression of p27 (Kip1), but resulted in a decrease in cyclin E, one of cyclins involved in G1 progression.Further, the apoptosis induction by PESV (40μg/mL) in DU145 cells was associated with an increase of pro-apoptotic protein Bax.

View Article: PubMed Central - PubMed

Affiliation: Key laboratory for Modern Medicine and Technology of Shandong Province, Institute of Basic Medicine, Shandong Academy of Medical Science, Jinan, China;

ABSTRACT

Background: Prostate cancer is a major cause of cancer-related death in men. Therefore there has been considerable interest to explore neoadjuvant therapy. Polypeptide extracted from scorpion venom (PESV), originally obtained from the East-Asian scorpion Buthus martensi Karsch (BmK), is being studied for both prevention and treatment of various human malignancies including prostate cancer.

Methods: The present study was to investigate the effect of PESV on cell proliferation, cell cycle, and apoptosis in human androgen-independent prostate cancer cells DU-145 in vitro.

Results: PESV treatment on these cells resulted in a significantly dose-dependent growth inhibition with a G1 phase arrest at 40μg/mL after 48h treatment. PESV treatment strongly induced expression of p27 (Kip1), but resulted in a decrease in cyclin E, one of cyclins involved in G1 progression. In other studies, PESV treatment also induced high apoptosis index (AI), confirmed by TdTmediated dUTP-biotin nick-end labeling (TUNEL) assay. Further, the apoptosis induction by PESV (40μg/mL) in DU145 cells was associated with an increase of pro-apoptotic protein Bax.

Conclusions: These results suggest that PESV modulates the expression of cell cycle-related and apoptosis-related proteins and induces growth inhibition and apoptosis of DU145 cells, providing a strong rationale for future studies to evaluate prevention or/and intervention strategies for PESV in pre-clinical prostate cancer models.

Keywords: Prostate cancer, PESV, cell proliferation, cell cycle, apoptosis.

No MeSH data available.


Related in: MedlinePlus

Grey Scale Intensity variants evaluated by Leica QWin V3 software of Cyclin E and p27 immunoreactivity in DU145 cells treated with PESV and control group. Higher grey scale intensity standing for weaker protein expression, and lower, sronger protein expression.
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Figure 6: Grey Scale Intensity variants evaluated by Leica QWin V3 software of Cyclin E and p27 immunoreactivity in DU145 cells treated with PESV and control group. Higher grey scale intensity standing for weaker protein expression, and lower, sronger protein expression.

Mentions: We determined the cell cycle phenotype of DU145 cell line by flow cytometry. As shown in Figure 4, DU145 cells exhibited PESV-associated G1/S arrest occurring at 48 h after exposure to PESV (40 mg/mL). Cells showed a lack of proliferation at 48 h in comparison with untreated controls (not shown), indicating that the PESV (40 mg/mL) caused proliferation arrest. We further detected the expression of cyclerelated protein cyclin E and p27 by immunohistochemical staining. Immunoreactivity was quantified within 5 random fields at 100x magnification. As shown in Fig. 5 and Fig. 6, cyclin E and p27(Kip1) immunostaining pattern was nuclear. DU145 cells exhibited lower expression of cyclin E protein and higher expression of p27(Kip1) protein after exposure to PESV (40 mg/mL) compared with untreated control.


Anti-proliferation Effect of Polypeptide Extracted from Scorpion Venom on Human Prostate Cancer Cells in vitro.

Zhang YY, Wu LC, Wang ZP, Wang ZX, Jia Q, Jiang GS, Zhang WD - J Clin Med Res (2009)

Grey Scale Intensity variants evaluated by Leica QWin V3 software of Cyclin E and p27 immunoreactivity in DU145 cells treated with PESV and control group. Higher grey scale intensity standing for weaker protein expression, and lower, sronger protein expression.
© Copyright Policy - open access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3318865&req=5

Figure 6: Grey Scale Intensity variants evaluated by Leica QWin V3 software of Cyclin E and p27 immunoreactivity in DU145 cells treated with PESV and control group. Higher grey scale intensity standing for weaker protein expression, and lower, sronger protein expression.
Mentions: We determined the cell cycle phenotype of DU145 cell line by flow cytometry. As shown in Figure 4, DU145 cells exhibited PESV-associated G1/S arrest occurring at 48 h after exposure to PESV (40 mg/mL). Cells showed a lack of proliferation at 48 h in comparison with untreated controls (not shown), indicating that the PESV (40 mg/mL) caused proliferation arrest. We further detected the expression of cyclerelated protein cyclin E and p27 by immunohistochemical staining. Immunoreactivity was quantified within 5 random fields at 100x magnification. As shown in Fig. 5 and Fig. 6, cyclin E and p27(Kip1) immunostaining pattern was nuclear. DU145 cells exhibited lower expression of cyclin E protein and higher expression of p27(Kip1) protein after exposure to PESV (40 mg/mL) compared with untreated control.

Bottom Line: PESV treatment on these cells resulted in a significantly dose-dependent growth inhibition with a G1 phase arrest at 40μg/mL after 48h treatment.PESV treatment strongly induced expression of p27 (Kip1), but resulted in a decrease in cyclin E, one of cyclins involved in G1 progression.Further, the apoptosis induction by PESV (40μg/mL) in DU145 cells was associated with an increase of pro-apoptotic protein Bax.

View Article: PubMed Central - PubMed

Affiliation: Key laboratory for Modern Medicine and Technology of Shandong Province, Institute of Basic Medicine, Shandong Academy of Medical Science, Jinan, China;

ABSTRACT

Background: Prostate cancer is a major cause of cancer-related death in men. Therefore there has been considerable interest to explore neoadjuvant therapy. Polypeptide extracted from scorpion venom (PESV), originally obtained from the East-Asian scorpion Buthus martensi Karsch (BmK), is being studied for both prevention and treatment of various human malignancies including prostate cancer.

Methods: The present study was to investigate the effect of PESV on cell proliferation, cell cycle, and apoptosis in human androgen-independent prostate cancer cells DU-145 in vitro.

Results: PESV treatment on these cells resulted in a significantly dose-dependent growth inhibition with a G1 phase arrest at 40μg/mL after 48h treatment. PESV treatment strongly induced expression of p27 (Kip1), but resulted in a decrease in cyclin E, one of cyclins involved in G1 progression. In other studies, PESV treatment also induced high apoptosis index (AI), confirmed by TdTmediated dUTP-biotin nick-end labeling (TUNEL) assay. Further, the apoptosis induction by PESV (40μg/mL) in DU145 cells was associated with an increase of pro-apoptotic protein Bax.

Conclusions: These results suggest that PESV modulates the expression of cell cycle-related and apoptosis-related proteins and induces growth inhibition and apoptosis of DU145 cells, providing a strong rationale for future studies to evaluate prevention or/and intervention strategies for PESV in pre-clinical prostate cancer models.

Keywords: Prostate cancer, PESV, cell proliferation, cell cycle, apoptosis.

No MeSH data available.


Related in: MedlinePlus