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Mate tea prevents oxidative stress in the blood and hippocampus of rats with acute or chronic ethanol administration.

Scolaro B, Delwing-de Lima D, da Cruz JG, Delwing-Dal Magro D - Oxid Med Cell Longev (2012)

Bottom Line: The aim of this study was to evaluate the influence of acute and chronic intake of mate tea on the effects elicited by acute and chronic administration of ethanol.Both ethanol administrations significantly increased TBARS in plasma and hippocampus of rats and altered antioxidant enzyme activities, changes which were reverted by mate tea administration.Data indicate that acute and chronic ethanol administration induced oxidative stress in hippocampus and blood and that mate tea treatment was able to prevent this situation.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciências Exatas e Naturais, Departamento de Ciências Naturais, Universidade Regional de Blumenau, Blumenau, SC, Brazil. deboradelwing@furb.br

ABSTRACT

Objective: The aim of this study was to evaluate the influence of acute and chronic intake of mate tea on the effects elicited by acute and chronic administration of ethanol.

Methods: Oxidative stress was evaluated by measuring thiobarbituric acid-reactive substances (TBARS), as well as the activities of the antioxidant enzymes, catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) in the hippocampus and blood of rats. Male Wistar rats were randomly assigned to four groups, for both acute and chronic treatment: (1) control group, (2) treated group, (3) intoxicated group, (4) and intoxicated group treated with mate tea.

Results: Both ethanol administrations significantly increased TBARS in plasma and hippocampus of rats and altered antioxidant enzyme activities, changes which were reverted by mate tea administration.

Conclusions: Data indicate that acute and chronic ethanol administration induced oxidative stress in hippocampus and blood and that mate tea treatment was able to prevent this situation.

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Related in: MedlinePlus

Effects of chronic administration of ethanol, mate tea, and mate tea plus ethanol on thiobarbituric acid reactive substances (TBA-RSs) (a), on catalase (b) and glutathione peroxidase (c) activities in rat hippocampus. Data are mean ± SD for 5 independent experiments (animals) performed in duplicate. *P < 0.05; **P < 0.01; ***P < 0.001 compared to control group (Duncan multiple-range test). One catalase (CAT) unit is defined as 1 μmol of H2O2 consumed per minute. One glutathione peroxidase (GSH-Px) unit is defined as 1 μmol of NADPH consumed per minute.
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fig3: Effects of chronic administration of ethanol, mate tea, and mate tea plus ethanol on thiobarbituric acid reactive substances (TBA-RSs) (a), on catalase (b) and glutathione peroxidase (c) activities in rat hippocampus. Data are mean ± SD for 5 independent experiments (animals) performed in duplicate. *P < 0.05; **P < 0.01; ***P < 0.001 compared to control group (Duncan multiple-range test). One catalase (CAT) unit is defined as 1 μmol of H2O2 consumed per minute. One glutathione peroxidase (GSH-Px) unit is defined as 1 μmol of NADPH consumed per minute.

Mentions: Subsequently, the effects of chronic ethanol, mate tea, and mate tea plus ethanol administration on TBARS and on the antioxidant enzymes activities of CAT, GSH-Px, and SOD were also evaluated in the hippocampus and plasma/erythrocytes of rats. As can be observed, Figure 3 shows that chronic ethanol administration significantly increased TBARS (48%) (a) and decreased CAT (21%) (b) and GSH-Px (8%) (c) activities in the hippocampus of rats and that mate tea pretreatment prevented the increase in TBARS (F(3,16) = 10.958; P < 0.001), as well as the decrease in CAT (F(3,16) = 3.247; P < 0.05) and GSH-PX (F(3,16) = 4.882; P < 0.01) activities in the rat hippocampus. SOD (t(8) = 1.471; P > 0.05) activity did not undergo any significant changes by chronic ethanol administration (result not shown). With regard to the chronic ethanol, mate tea and mate tea plus ethanol administration in the plasma/erythrocytes of rats, Figure 4 shows that chronic ethanol administration significantly increased TBARS (52%) (a) and SOD (38%) (b) activity as well as decreased CAT (42%) (c) and GSH-PX (13%) (d) activities in blood of rats. In addition, pretreatment with mate tea prevented the increase in TBARS (F(3,16) = 9.043; P < 0.001) in the plasma of rats, prevented the increase in SOD (F(3,16) = 9.032; P < 0.001) activity in erythrocytes, and also abolished the reductions in CAT (F(3,16) = 10.261; P < 0.001) and GSH-Px (F(3,16) = 28.783; P < 0.01) activities in erythrocytes, caused by chronic ethanol administration, respectively. Statistical analyses demonstrated that acute and chronic mate tea administration per se did not interfere in most of the parameters studied.


Mate tea prevents oxidative stress in the blood and hippocampus of rats with acute or chronic ethanol administration.

Scolaro B, Delwing-de Lima D, da Cruz JG, Delwing-Dal Magro D - Oxid Med Cell Longev (2012)

Effects of chronic administration of ethanol, mate tea, and mate tea plus ethanol on thiobarbituric acid reactive substances (TBA-RSs) (a), on catalase (b) and glutathione peroxidase (c) activities in rat hippocampus. Data are mean ± SD for 5 independent experiments (animals) performed in duplicate. *P < 0.05; **P < 0.01; ***P < 0.001 compared to control group (Duncan multiple-range test). One catalase (CAT) unit is defined as 1 μmol of H2O2 consumed per minute. One glutathione peroxidase (GSH-Px) unit is defined as 1 μmol of NADPH consumed per minute.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3316984&req=5

fig3: Effects of chronic administration of ethanol, mate tea, and mate tea plus ethanol on thiobarbituric acid reactive substances (TBA-RSs) (a), on catalase (b) and glutathione peroxidase (c) activities in rat hippocampus. Data are mean ± SD for 5 independent experiments (animals) performed in duplicate. *P < 0.05; **P < 0.01; ***P < 0.001 compared to control group (Duncan multiple-range test). One catalase (CAT) unit is defined as 1 μmol of H2O2 consumed per minute. One glutathione peroxidase (GSH-Px) unit is defined as 1 μmol of NADPH consumed per minute.
Mentions: Subsequently, the effects of chronic ethanol, mate tea, and mate tea plus ethanol administration on TBARS and on the antioxidant enzymes activities of CAT, GSH-Px, and SOD were also evaluated in the hippocampus and plasma/erythrocytes of rats. As can be observed, Figure 3 shows that chronic ethanol administration significantly increased TBARS (48%) (a) and decreased CAT (21%) (b) and GSH-Px (8%) (c) activities in the hippocampus of rats and that mate tea pretreatment prevented the increase in TBARS (F(3,16) = 10.958; P < 0.001), as well as the decrease in CAT (F(3,16) = 3.247; P < 0.05) and GSH-PX (F(3,16) = 4.882; P < 0.01) activities in the rat hippocampus. SOD (t(8) = 1.471; P > 0.05) activity did not undergo any significant changes by chronic ethanol administration (result not shown). With regard to the chronic ethanol, mate tea and mate tea plus ethanol administration in the plasma/erythrocytes of rats, Figure 4 shows that chronic ethanol administration significantly increased TBARS (52%) (a) and SOD (38%) (b) activity as well as decreased CAT (42%) (c) and GSH-PX (13%) (d) activities in blood of rats. In addition, pretreatment with mate tea prevented the increase in TBARS (F(3,16) = 9.043; P < 0.001) in the plasma of rats, prevented the increase in SOD (F(3,16) = 9.032; P < 0.001) activity in erythrocytes, and also abolished the reductions in CAT (F(3,16) = 10.261; P < 0.001) and GSH-Px (F(3,16) = 28.783; P < 0.01) activities in erythrocytes, caused by chronic ethanol administration, respectively. Statistical analyses demonstrated that acute and chronic mate tea administration per se did not interfere in most of the parameters studied.

Bottom Line: The aim of this study was to evaluate the influence of acute and chronic intake of mate tea on the effects elicited by acute and chronic administration of ethanol.Both ethanol administrations significantly increased TBARS in plasma and hippocampus of rats and altered antioxidant enzyme activities, changes which were reverted by mate tea administration.Data indicate that acute and chronic ethanol administration induced oxidative stress in hippocampus and blood and that mate tea treatment was able to prevent this situation.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciências Exatas e Naturais, Departamento de Ciências Naturais, Universidade Regional de Blumenau, Blumenau, SC, Brazil. deboradelwing@furb.br

ABSTRACT

Objective: The aim of this study was to evaluate the influence of acute and chronic intake of mate tea on the effects elicited by acute and chronic administration of ethanol.

Methods: Oxidative stress was evaluated by measuring thiobarbituric acid-reactive substances (TBARS), as well as the activities of the antioxidant enzymes, catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) in the hippocampus and blood of rats. Male Wistar rats were randomly assigned to four groups, for both acute and chronic treatment: (1) control group, (2) treated group, (3) intoxicated group, (4) and intoxicated group treated with mate tea.

Results: Both ethanol administrations significantly increased TBARS in plasma and hippocampus of rats and altered antioxidant enzyme activities, changes which were reverted by mate tea administration.

Conclusions: Data indicate that acute and chronic ethanol administration induced oxidative stress in hippocampus and blood and that mate tea treatment was able to prevent this situation.

Show MeSH
Related in: MedlinePlus