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Mate tea prevents oxidative stress in the blood and hippocampus of rats with acute or chronic ethanol administration.

Scolaro B, Delwing-de Lima D, da Cruz JG, Delwing-Dal Magro D - Oxid Med Cell Longev (2012)

Bottom Line: The aim of this study was to evaluate the influence of acute and chronic intake of mate tea on the effects elicited by acute and chronic administration of ethanol.Both ethanol administrations significantly increased TBARS in plasma and hippocampus of rats and altered antioxidant enzyme activities, changes which were reverted by mate tea administration.Data indicate that acute and chronic ethanol administration induced oxidative stress in hippocampus and blood and that mate tea treatment was able to prevent this situation.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciências Exatas e Naturais, Departamento de Ciências Naturais, Universidade Regional de Blumenau, Blumenau, SC, Brazil. deboradelwing@furb.br

ABSTRACT

Objective: The aim of this study was to evaluate the influence of acute and chronic intake of mate tea on the effects elicited by acute and chronic administration of ethanol.

Methods: Oxidative stress was evaluated by measuring thiobarbituric acid-reactive substances (TBARS), as well as the activities of the antioxidant enzymes, catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) in the hippocampus and blood of rats. Male Wistar rats were randomly assigned to four groups, for both acute and chronic treatment: (1) control group, (2) treated group, (3) intoxicated group, (4) and intoxicated group treated with mate tea.

Results: Both ethanol administrations significantly increased TBARS in plasma and hippocampus of rats and altered antioxidant enzyme activities, changes which were reverted by mate tea administration.

Conclusions: Data indicate that acute and chronic ethanol administration induced oxidative stress in hippocampus and blood and that mate tea treatment was able to prevent this situation.

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Related in: MedlinePlus

Effects of acute administration of ethanol, mate tea, and mate tea plus ethanol on thiobarbituric acid reactive substances (TBA-RSs) (a), on catalase (b) and superoxide dismutase (c) activities in rat hippocampus. Data are mean ± SD for 5 independent experiments (animals) performed in duplicate. **P < 0.01; ***P < 0.001 compared to control group (Duncan multiple-range test). One catalase (CAT) unit is defined as 1 μmol of H2O2 consumed per minute. One superoxide dismutase (SOD) unit is defined as the amount of SOD necessary to inhibit 50% of pyrogallol autoxidation.
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fig1: Effects of acute administration of ethanol, mate tea, and mate tea plus ethanol on thiobarbituric acid reactive substances (TBA-RSs) (a), on catalase (b) and superoxide dismutase (c) activities in rat hippocampus. Data are mean ± SD for 5 independent experiments (animals) performed in duplicate. **P < 0.01; ***P < 0.001 compared to control group (Duncan multiple-range test). One catalase (CAT) unit is defined as 1 μmol of H2O2 consumed per minute. One superoxide dismutase (SOD) unit is defined as the amount of SOD necessary to inhibit 50% of pyrogallol autoxidation.

Mentions: We first studied the effects of acute ethanol, mate tea, and mate tea plus ethanol administration on an important parameter of lipoperoxidation, namely, TBARS and on the antioxidant enzymes activities of CAT, GSH-Px, and SOD in the hippocampus and blood of rats. Figure 1 shows that acute ethanol administration significantly increased TBARS (81%) (a) as well as decreased CAT (40%) (b) and SOD (40%) (c) activities in the hippocampus of rats and that acute mate tea administration prevented the increase in TBARS (F(3,16) = 12.895; P < 0.001), as well as the reduction of CAT (F(3,16) = 4.795; P < 0.01) and SOD (F(3,16) = 11.825; P < 0.001) activity, caused by acute ethanol administration, when compared to the control group. However acute ethanol administration had no effect on GSH-Px (t(8) = 0.490; P > 0.05) activity, when compared to the control group (result not shown). As can be observed in Figure 2, acute ethanol administration significantly increased TBARS levels (35%) (a) and CAT (61%) (b) activity in plasma and erythrocytes of rats, respectively, while acute mate tea administration was able to prevent the increase in TBARS (F(3,16) = 11.898; P < 0.001), caused by ethanol, in the plasma of rats. In contrast, such prevention was not observed for the increased CAT activity observed in the erythrocytes of rats (F(3,16) = 5.809; P < 0.01). In addition, GSH-Px (t(8) = 0.60; P > 0.05) and SOD (t(8) = −1.714; P > 0.05) activities did not undergo any significant alteration by acute ethanol administration, when compared to the control groups (results not shown).


Mate tea prevents oxidative stress in the blood and hippocampus of rats with acute or chronic ethanol administration.

Scolaro B, Delwing-de Lima D, da Cruz JG, Delwing-Dal Magro D - Oxid Med Cell Longev (2012)

Effects of acute administration of ethanol, mate tea, and mate tea plus ethanol on thiobarbituric acid reactive substances (TBA-RSs) (a), on catalase (b) and superoxide dismutase (c) activities in rat hippocampus. Data are mean ± SD for 5 independent experiments (animals) performed in duplicate. **P < 0.01; ***P < 0.001 compared to control group (Duncan multiple-range test). One catalase (CAT) unit is defined as 1 μmol of H2O2 consumed per minute. One superoxide dismutase (SOD) unit is defined as the amount of SOD necessary to inhibit 50% of pyrogallol autoxidation.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3316984&req=5

fig1: Effects of acute administration of ethanol, mate tea, and mate tea plus ethanol on thiobarbituric acid reactive substances (TBA-RSs) (a), on catalase (b) and superoxide dismutase (c) activities in rat hippocampus. Data are mean ± SD for 5 independent experiments (animals) performed in duplicate. **P < 0.01; ***P < 0.001 compared to control group (Duncan multiple-range test). One catalase (CAT) unit is defined as 1 μmol of H2O2 consumed per minute. One superoxide dismutase (SOD) unit is defined as the amount of SOD necessary to inhibit 50% of pyrogallol autoxidation.
Mentions: We first studied the effects of acute ethanol, mate tea, and mate tea plus ethanol administration on an important parameter of lipoperoxidation, namely, TBARS and on the antioxidant enzymes activities of CAT, GSH-Px, and SOD in the hippocampus and blood of rats. Figure 1 shows that acute ethanol administration significantly increased TBARS (81%) (a) as well as decreased CAT (40%) (b) and SOD (40%) (c) activities in the hippocampus of rats and that acute mate tea administration prevented the increase in TBARS (F(3,16) = 12.895; P < 0.001), as well as the reduction of CAT (F(3,16) = 4.795; P < 0.01) and SOD (F(3,16) = 11.825; P < 0.001) activity, caused by acute ethanol administration, when compared to the control group. However acute ethanol administration had no effect on GSH-Px (t(8) = 0.490; P > 0.05) activity, when compared to the control group (result not shown). As can be observed in Figure 2, acute ethanol administration significantly increased TBARS levels (35%) (a) and CAT (61%) (b) activity in plasma and erythrocytes of rats, respectively, while acute mate tea administration was able to prevent the increase in TBARS (F(3,16) = 11.898; P < 0.001), caused by ethanol, in the plasma of rats. In contrast, such prevention was not observed for the increased CAT activity observed in the erythrocytes of rats (F(3,16) = 5.809; P < 0.01). In addition, GSH-Px (t(8) = 0.60; P > 0.05) and SOD (t(8) = −1.714; P > 0.05) activities did not undergo any significant alteration by acute ethanol administration, when compared to the control groups (results not shown).

Bottom Line: The aim of this study was to evaluate the influence of acute and chronic intake of mate tea on the effects elicited by acute and chronic administration of ethanol.Both ethanol administrations significantly increased TBARS in plasma and hippocampus of rats and altered antioxidant enzyme activities, changes which were reverted by mate tea administration.Data indicate that acute and chronic ethanol administration induced oxidative stress in hippocampus and blood and that mate tea treatment was able to prevent this situation.

View Article: PubMed Central - PubMed

Affiliation: Centro de Ciências Exatas e Naturais, Departamento de Ciências Naturais, Universidade Regional de Blumenau, Blumenau, SC, Brazil. deboradelwing@furb.br

ABSTRACT

Objective: The aim of this study was to evaluate the influence of acute and chronic intake of mate tea on the effects elicited by acute and chronic administration of ethanol.

Methods: Oxidative stress was evaluated by measuring thiobarbituric acid-reactive substances (TBARS), as well as the activities of the antioxidant enzymes, catalase (CAT), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) in the hippocampus and blood of rats. Male Wistar rats were randomly assigned to four groups, for both acute and chronic treatment: (1) control group, (2) treated group, (3) intoxicated group, (4) and intoxicated group treated with mate tea.

Results: Both ethanol administrations significantly increased TBARS in plasma and hippocampus of rats and altered antioxidant enzyme activities, changes which were reverted by mate tea administration.

Conclusions: Data indicate that acute and chronic ethanol administration induced oxidative stress in hippocampus and blood and that mate tea treatment was able to prevent this situation.

Show MeSH
Related in: MedlinePlus