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Evaluation of oxidative stress and antioxidant status in patients with cardiovascular disease in rural populations of the nilgiris, South India.

Kumar EP, Mukherjee R, Senthil R, Parasuraman S, Suresh B - ISRN Pharmacol (2012)

Bottom Line: Significantly reduced antioxidant levels and increased TBARS levels were found in the intervention group compared with the control group.Statistically significant differences were not found in the antioxidant and TBARS levels when comparing smokers versus nonsmokers, alcoholics versus nonalcoholics, and vegetarians versus nonvegetarians.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, JSS College of Pharmacy, Ootacamund 643001, India.

ABSTRACT
Objective. The objective of this work was to study the risk factors of ischaemic heart disease (IHD) in rural populations of the Nilgiris, south India, with stress on the various social habits and oxidant stress. Methods. A total of 72 patients with cardiovascular disease (CVD) and 12 healthy volunteers were screened. Forty-seven patients with CVD (intervention group) and 10 healthy volunteers (control group) were randomly selected for the study. Written informed consent was obtained from all the participants, and their demographic details were collected. A 6 mL blood sample was collected from each of the participants, and the serum was separated in the samples. The levels of enzymic (superoxide dismutase, catalase) and nonenzymic antioxidants (ascorbic acid) in the plasma were determined biochemically. The level of thiobarbituric acid species (TBARS), which is a predictor of lipid peroxidation, was measured. Results. The participants of the study were stratified as according to demographic and social variables. The values of all the antioxidants and TBARS were statistically compared. Significantly reduced antioxidant levels and increased TBARS levels were found in the intervention group compared with the control group. The results suggest that the lowered antioxidant level may be a result of the oxidant stress of the disease. Statistically significant differences were not found in the antioxidant and TBARS levels when comparing smokers versus nonsmokers, alcoholics versus nonalcoholics, and vegetarians versus nonvegetarians. Conclusion. The major causes of CVD amongst the rural populations of the Nilgiris, south India, are preventable causes such as smoking and high fat intake, all of which cause oxidative stress, as seen in our study through various serum markers.

No MeSH data available.


Related in: MedlinePlus

Requirement, allocation, and follow-up of participants.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig1: Requirement, allocation, and follow-up of participants.

Mentions: A 6 mL fasting blood sample was collected from each participant. The blood samples were centrifuged at 3000 RPM for 20 minutes, and the serum was separated and stored at −20°C until analysis. The levels of enzymic (superoxide dismutase (SOD) [9] and catalase (CAT) [10]) and nonenzymic (ascorbic acid [11]) antioxidants and predictor of lipid peroxidation as measured by the thiobarbituric acid species (TBARS) [12] in the serum were determined. Flow diagram of requirement and allocation of participants and analysis of the data are given in Figure 1.


Evaluation of oxidative stress and antioxidant status in patients with cardiovascular disease in rural populations of the nilgiris, South India.

Kumar EP, Mukherjee R, Senthil R, Parasuraman S, Suresh B - ISRN Pharmacol (2012)

Requirement, allocation, and follow-up of participants.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3316956&req=5

fig1: Requirement, allocation, and follow-up of participants.
Mentions: A 6 mL fasting blood sample was collected from each participant. The blood samples were centrifuged at 3000 RPM for 20 minutes, and the serum was separated and stored at −20°C until analysis. The levels of enzymic (superoxide dismutase (SOD) [9] and catalase (CAT) [10]) and nonenzymic (ascorbic acid [11]) antioxidants and predictor of lipid peroxidation as measured by the thiobarbituric acid species (TBARS) [12] in the serum were determined. Flow diagram of requirement and allocation of participants and analysis of the data are given in Figure 1.

Bottom Line: Significantly reduced antioxidant levels and increased TBARS levels were found in the intervention group compared with the control group.Statistically significant differences were not found in the antioxidant and TBARS levels when comparing smokers versus nonsmokers, alcoholics versus nonalcoholics, and vegetarians versus nonvegetarians.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, JSS College of Pharmacy, Ootacamund 643001, India.

ABSTRACT
Objective. The objective of this work was to study the risk factors of ischaemic heart disease (IHD) in rural populations of the Nilgiris, south India, with stress on the various social habits and oxidant stress. Methods. A total of 72 patients with cardiovascular disease (CVD) and 12 healthy volunteers were screened. Forty-seven patients with CVD (intervention group) and 10 healthy volunteers (control group) were randomly selected for the study. Written informed consent was obtained from all the participants, and their demographic details were collected. A 6 mL blood sample was collected from each of the participants, and the serum was separated in the samples. The levels of enzymic (superoxide dismutase, catalase) and nonenzymic antioxidants (ascorbic acid) in the plasma were determined biochemically. The level of thiobarbituric acid species (TBARS), which is a predictor of lipid peroxidation, was measured. Results. The participants of the study were stratified as according to demographic and social variables. The values of all the antioxidants and TBARS were statistically compared. Significantly reduced antioxidant levels and increased TBARS levels were found in the intervention group compared with the control group. The results suggest that the lowered antioxidant level may be a result of the oxidant stress of the disease. Statistically significant differences were not found in the antioxidant and TBARS levels when comparing smokers versus nonsmokers, alcoholics versus nonalcoholics, and vegetarians versus nonvegetarians. Conclusion. The major causes of CVD amongst the rural populations of the Nilgiris, south India, are preventable causes such as smoking and high fat intake, all of which cause oxidative stress, as seen in our study through various serum markers.

No MeSH data available.


Related in: MedlinePlus