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A crucial role of IL-17 and IFN-γ during acute rejection of peripheral nerve xenotransplantation in mice.

Yu X, Jiang Y, Lu L, Gong X, Sun X, Xuan Z, Lu L - PLoS ONE (2012)

Bottom Line: However, xenotransplant rejection is severe with cellular immunity, and Th1 cells play an important role in the process.The changes of IL-4 level had no significant difference between xenotransplanted group and sham control group.These data suggest that Th17 cells contribute to the acute rejection process of peripheral nerve xenotransplant in addition to Th1 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Hand Surgery, First Hospital, Jilin University, Changchun, China.

ABSTRACT
Nerve injuries causing segmental loss require nerve grafting. However, autografts and allografts have limitations for clinical use. Peripheral nerve xenotransplantation has become an area of great interest in clinical surgery research as an alternative graft strategy. However, xenotransplant rejection is severe with cellular immunity, and Th1 cells play an important role in the process. To better understand the process of rejection, we used peripheral nerve xenografts from rats to mice and found that mononuclear cells expressing IFN-γ and IL-17 infiltrated around the grafts, and IFN-γ and IL-17 producing CD4+ and CD8+ T cells increased during the process of acute rejection. The changes of IL-4 level had no significant difference between xenotransplanted group and sham control group. The rejection of xenograft was significantly prevented after the treatment of IL-17 and IFN-γ neutralizing antibodies. These data suggest that Th17 cells contribute to the acute rejection process of peripheral nerve xenotransplant in addition to Th1 cells.

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ELISA assays of IFN-γ, IL-17 and IL-4 in serum.Sera from collected blood at each time point (1, 3, 6, 11, 16, and 30 days) from xenotransplanted and sham control mice were used for ELISA analysis. Sera IFN-γ and IL-17 levels in the xenograft group were significantly higher than those in the serum in the control group (p<0.05) at day 1, day 3 and day 6, and decreased to control levels by day 11. There is no significant difference of IL-4 levels between recipients and control group. *, p<0.05; **, p<0.01; *** p<0.001. (We performed this experiment twice).
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pone-0034419-g003: ELISA assays of IFN-γ, IL-17 and IL-4 in serum.Sera from collected blood at each time point (1, 3, 6, 11, 16, and 30 days) from xenotransplanted and sham control mice were used for ELISA analysis. Sera IFN-γ and IL-17 levels in the xenograft group were significantly higher than those in the serum in the control group (p<0.05) at day 1, day 3 and day 6, and decreased to control levels by day 11. There is no significant difference of IL-4 levels between recipients and control group. *, p<0.05; **, p<0.01; *** p<0.001. (We performed this experiment twice).

Mentions: To provide a more quantitative assessment of changes in IFN-γ and IL-17 during rejection, concentrations of both cytokines were quantified by ELISA from sera of xenograft and sham control animals. The sera levels of IFN-γ and IL-17 at day 1, 3, and 6 were statistically higher in the xenograft recipients compared to the control group. The levels of both cytokines returned to control values by day 11 after transplantation. The IL-4 levels in sera did not show significant changes between the recipients with xenograft transplantation and the control group (Fig. 3).


A crucial role of IL-17 and IFN-γ during acute rejection of peripheral nerve xenotransplantation in mice.

Yu X, Jiang Y, Lu L, Gong X, Sun X, Xuan Z, Lu L - PLoS ONE (2012)

ELISA assays of IFN-γ, IL-17 and IL-4 in serum.Sera from collected blood at each time point (1, 3, 6, 11, 16, and 30 days) from xenotransplanted and sham control mice were used for ELISA analysis. Sera IFN-γ and IL-17 levels in the xenograft group were significantly higher than those in the serum in the control group (p<0.05) at day 1, day 3 and day 6, and decreased to control levels by day 11. There is no significant difference of IL-4 levels between recipients and control group. *, p<0.05; **, p<0.01; *** p<0.001. (We performed this experiment twice).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3316676&req=5

pone-0034419-g003: ELISA assays of IFN-γ, IL-17 and IL-4 in serum.Sera from collected blood at each time point (1, 3, 6, 11, 16, and 30 days) from xenotransplanted and sham control mice were used for ELISA analysis. Sera IFN-γ and IL-17 levels in the xenograft group were significantly higher than those in the serum in the control group (p<0.05) at day 1, day 3 and day 6, and decreased to control levels by day 11. There is no significant difference of IL-4 levels between recipients and control group. *, p<0.05; **, p<0.01; *** p<0.001. (We performed this experiment twice).
Mentions: To provide a more quantitative assessment of changes in IFN-γ and IL-17 during rejection, concentrations of both cytokines were quantified by ELISA from sera of xenograft and sham control animals. The sera levels of IFN-γ and IL-17 at day 1, 3, and 6 were statistically higher in the xenograft recipients compared to the control group. The levels of both cytokines returned to control values by day 11 after transplantation. The IL-4 levels in sera did not show significant changes between the recipients with xenograft transplantation and the control group (Fig. 3).

Bottom Line: However, xenotransplant rejection is severe with cellular immunity, and Th1 cells play an important role in the process.The changes of IL-4 level had no significant difference between xenotransplanted group and sham control group.These data suggest that Th17 cells contribute to the acute rejection process of peripheral nerve xenotransplant in addition to Th1 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Hand Surgery, First Hospital, Jilin University, Changchun, China.

ABSTRACT
Nerve injuries causing segmental loss require nerve grafting. However, autografts and allografts have limitations for clinical use. Peripheral nerve xenotransplantation has become an area of great interest in clinical surgery research as an alternative graft strategy. However, xenotransplant rejection is severe with cellular immunity, and Th1 cells play an important role in the process. To better understand the process of rejection, we used peripheral nerve xenografts from rats to mice and found that mononuclear cells expressing IFN-γ and IL-17 infiltrated around the grafts, and IFN-γ and IL-17 producing CD4+ and CD8+ T cells increased during the process of acute rejection. The changes of IL-4 level had no significant difference between xenotransplanted group and sham control group. The rejection of xenograft was significantly prevented after the treatment of IL-17 and IFN-γ neutralizing antibodies. These data suggest that Th17 cells contribute to the acute rejection process of peripheral nerve xenotransplant in addition to Th1 cells.

Show MeSH
Related in: MedlinePlus