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A crucial role of IL-17 and IFN-γ during acute rejection of peripheral nerve xenotransplantation in mice.

Yu X, Jiang Y, Lu L, Gong X, Sun X, Xuan Z, Lu L - PLoS ONE (2012)

Bottom Line: However, xenotransplant rejection is severe with cellular immunity, and Th1 cells play an important role in the process.The changes of IL-4 level had no significant difference between xenotransplanted group and sham control group.These data suggest that Th17 cells contribute to the acute rejection process of peripheral nerve xenotransplant in addition to Th1 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Hand Surgery, First Hospital, Jilin University, Changchun, China.

ABSTRACT
Nerve injuries causing segmental loss require nerve grafting. However, autografts and allografts have limitations for clinical use. Peripheral nerve xenotransplantation has become an area of great interest in clinical surgery research as an alternative graft strategy. However, xenotransplant rejection is severe with cellular immunity, and Th1 cells play an important role in the process. To better understand the process of rejection, we used peripheral nerve xenografts from rats to mice and found that mononuclear cells expressing IFN-γ and IL-17 infiltrated around the grafts, and IFN-γ and IL-17 producing CD4+ and CD8+ T cells increased during the process of acute rejection. The changes of IL-4 level had no significant difference between xenotransplanted group and sham control group. The rejection of xenograft was significantly prevented after the treatment of IL-17 and IFN-γ neutralizing antibodies. These data suggest that Th17 cells contribute to the acute rejection process of peripheral nerve xenotransplant in addition to Th1 cells.

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H&E staining of nerve grafts.Sham control or xenotransplanted sciatic nerve from mice was collected at 1, 3, 6, 11, 16, and 30 days posttransplantation, sectioned, and stained with H&E. Staining demonstrated xenograft adhesion to the surrounding tissue, and infiltration of mononuclear cells into the peripheral nerve and surrounding tissue. There was no significant difference during 30 days after xenotransplantation in control group. The level of mononuclear cell infiltration, distension, and necrosis significantly increased in all xenografts at all time point after transplantation compared with control group. Magnification: 200×. (We performed animal experiment twice, and stained 2 sections for each animal.)
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pone-0034419-g001: H&E staining of nerve grafts.Sham control or xenotransplanted sciatic nerve from mice was collected at 1, 3, 6, 11, 16, and 30 days posttransplantation, sectioned, and stained with H&E. Staining demonstrated xenograft adhesion to the surrounding tissue, and infiltration of mononuclear cells into the peripheral nerve and surrounding tissue. There was no significant difference during 30 days after xenotransplantation in control group. The level of mononuclear cell infiltration, distension, and necrosis significantly increased in all xenografts at all time point after transplantation compared with control group. Magnification: 200×. (We performed animal experiment twice, and stained 2 sections for each animal.)

Mentions: In order to examine the general cellular changes around the grafts during rejection, the tissue was collected, sectioned and visualized using routine H&E staining. Compared to the control group, we observed that xenograft adhesion to the surrounding tissue was progressively overwhelmed in all mice during the 30-day observation period in all grafted animals. Histologic evidence of rejection was identified in all grafted tissue samples. There was no significant difference during 30 days after xenotransplantation in control group. The level of mononuclear cell infiltration, distension, and necrosis significantly increased in all xenografts at different time point after xenotransplantation compared with the control group. (Fig. 1).


A crucial role of IL-17 and IFN-γ during acute rejection of peripheral nerve xenotransplantation in mice.

Yu X, Jiang Y, Lu L, Gong X, Sun X, Xuan Z, Lu L - PLoS ONE (2012)

H&E staining of nerve grafts.Sham control or xenotransplanted sciatic nerve from mice was collected at 1, 3, 6, 11, 16, and 30 days posttransplantation, sectioned, and stained with H&E. Staining demonstrated xenograft adhesion to the surrounding tissue, and infiltration of mononuclear cells into the peripheral nerve and surrounding tissue. There was no significant difference during 30 days after xenotransplantation in control group. The level of mononuclear cell infiltration, distension, and necrosis significantly increased in all xenografts at all time point after transplantation compared with control group. Magnification: 200×. (We performed animal experiment twice, and stained 2 sections for each animal.)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3316676&req=5

pone-0034419-g001: H&E staining of nerve grafts.Sham control or xenotransplanted sciatic nerve from mice was collected at 1, 3, 6, 11, 16, and 30 days posttransplantation, sectioned, and stained with H&E. Staining demonstrated xenograft adhesion to the surrounding tissue, and infiltration of mononuclear cells into the peripheral nerve and surrounding tissue. There was no significant difference during 30 days after xenotransplantation in control group. The level of mononuclear cell infiltration, distension, and necrosis significantly increased in all xenografts at all time point after transplantation compared with control group. Magnification: 200×. (We performed animal experiment twice, and stained 2 sections for each animal.)
Mentions: In order to examine the general cellular changes around the grafts during rejection, the tissue was collected, sectioned and visualized using routine H&E staining. Compared to the control group, we observed that xenograft adhesion to the surrounding tissue was progressively overwhelmed in all mice during the 30-day observation period in all grafted animals. Histologic evidence of rejection was identified in all grafted tissue samples. There was no significant difference during 30 days after xenotransplantation in control group. The level of mononuclear cell infiltration, distension, and necrosis significantly increased in all xenografts at different time point after xenotransplantation compared with the control group. (Fig. 1).

Bottom Line: However, xenotransplant rejection is severe with cellular immunity, and Th1 cells play an important role in the process.The changes of IL-4 level had no significant difference between xenotransplanted group and sham control group.These data suggest that Th17 cells contribute to the acute rejection process of peripheral nerve xenotransplant in addition to Th1 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Hand Surgery, First Hospital, Jilin University, Changchun, China.

ABSTRACT
Nerve injuries causing segmental loss require nerve grafting. However, autografts and allografts have limitations for clinical use. Peripheral nerve xenotransplantation has become an area of great interest in clinical surgery research as an alternative graft strategy. However, xenotransplant rejection is severe with cellular immunity, and Th1 cells play an important role in the process. To better understand the process of rejection, we used peripheral nerve xenografts from rats to mice and found that mononuclear cells expressing IFN-γ and IL-17 infiltrated around the grafts, and IFN-γ and IL-17 producing CD4+ and CD8+ T cells increased during the process of acute rejection. The changes of IL-4 level had no significant difference between xenotransplanted group and sham control group. The rejection of xenograft was significantly prevented after the treatment of IL-17 and IFN-γ neutralizing antibodies. These data suggest that Th17 cells contribute to the acute rejection process of peripheral nerve xenotransplant in addition to Th1 cells.

Show MeSH
Related in: MedlinePlus