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Molecular cloning and in silico analysis of the duck (Anas platyrhynchos) MEF2A gene cDNA and its expression profile in muscle tissues during fetal development.

Liu H, Wang J, Si J, Jia J, Li L, Han C, Huang K, He H, Xu F - Genet. Mol. Biol. (2012)

Bottom Line: Modified sites in these domains were conserved among species and several variants were found.These results indicate that the conserved domains of duck MEF2A, including the MADS and MEF2 domains, are important for MEF2A transcription factor function.The expression of MEF2A in duck smooth muscle and cardiac muscle suggests that MEF2A plays a role in these two tissues.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, P.R. China.

ABSTRACT
The role of myogenic enhancer transcription factor 2a (MEF2A) in avian muscle during fetal development is unknown. In this work, we cloned the duck MEF2A cDNA sequence (GenBank accession no. HM460752) and examined its developmental expression profiles in cardiac muscle, non-vascular smooth muscle and skeletal muscle. Duck MEF2A cDNA comprised 1479 bp encoding 492 amino acid residues. In silico analysis showed that MEF2A contained MADS (MCM1, AGAMOUS, DEFICIENS and SRF - serum response factor), MEF2 and mitogen-activated protein kinase (MAPK) transcription domains with high homology to related proteins in other species. Modified sites in these domains were conserved among species and several variants were found. Quantitative PCR showed that MEF2A was expressed in all three muscles at each developmental stage examined, with the expression in smooth muscle being higher than in the other muscles. These results indicate that the conserved domains of duck MEF2A, including the MADS and MEF2 domains, are important for MEF2A transcription factor function. The expression of MEF2A in duck smooth muscle and cardiac muscle suggests that MEF2A plays a role in these two tissues.

No MeSH data available.


Secondary structure prediction of the D1, D2, D3 and V1 regions in Figure 1 and prediction of processing using the protean program in DNAstar software, based on the Chou-Fasman mathematic model. The numbers in the figure represent amino acid positions. A - α helix, B - β sheet and T - random turn.
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f3-gmb-35-1-182: Secondary structure prediction of the D1, D2, D3 and V1 regions in Figure 1 and prediction of processing using the protean program in DNAstar software, based on the Chou-Fasman mathematic model. The numbers in the figure represent amino acid positions. A - α helix, B - β sheet and T - random turn.

Mentions: Several modified residues were identified in MEF2A in addition to the conserved domains of MEF2A. Most of these were conserved among species, including acetyllysine sites at positions 117, 248, 253 and 394, phosphoserine sites at positions 98, 221, 233, 346, 399 and 439, and phosphothreonine sites at positions 302 and 310. Several modified sites differed between birds and mammals, such as the phosphoserine sites at positions 148, 153, 175, 176, 208 and 267. The modified sites could be grouped into four regions, identified as regions 1, 2 and 3 (D1, D2, and D3, respectively) and a region of variants 1 (V1) (Figure 3). The D1–D3 regions had the same characteristics as avian (duck and chick) MEF2A, which differed from those of mammals. The V1 region varied in all of the species examined. The secondary structures of the D1–D3 and V1 regions were predicted using the protean program in DANstar, and the results showed that the secondary structure distributions in the D1–D3 regions differed between birds and mammals (Figure 3).


Molecular cloning and in silico analysis of the duck (Anas platyrhynchos) MEF2A gene cDNA and its expression profile in muscle tissues during fetal development.

Liu H, Wang J, Si J, Jia J, Li L, Han C, Huang K, He H, Xu F - Genet. Mol. Biol. (2012)

Secondary structure prediction of the D1, D2, D3 and V1 regions in Figure 1 and prediction of processing using the protean program in DNAstar software, based on the Chou-Fasman mathematic model. The numbers in the figure represent amino acid positions. A - α helix, B - β sheet and T - random turn.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3313510&req=5

f3-gmb-35-1-182: Secondary structure prediction of the D1, D2, D3 and V1 regions in Figure 1 and prediction of processing using the protean program in DNAstar software, based on the Chou-Fasman mathematic model. The numbers in the figure represent amino acid positions. A - α helix, B - β sheet and T - random turn.
Mentions: Several modified residues were identified in MEF2A in addition to the conserved domains of MEF2A. Most of these were conserved among species, including acetyllysine sites at positions 117, 248, 253 and 394, phosphoserine sites at positions 98, 221, 233, 346, 399 and 439, and phosphothreonine sites at positions 302 and 310. Several modified sites differed between birds and mammals, such as the phosphoserine sites at positions 148, 153, 175, 176, 208 and 267. The modified sites could be grouped into four regions, identified as regions 1, 2 and 3 (D1, D2, and D3, respectively) and a region of variants 1 (V1) (Figure 3). The D1–D3 regions had the same characteristics as avian (duck and chick) MEF2A, which differed from those of mammals. The V1 region varied in all of the species examined. The secondary structures of the D1–D3 and V1 regions were predicted using the protean program in DANstar, and the results showed that the secondary structure distributions in the D1–D3 regions differed between birds and mammals (Figure 3).

Bottom Line: Modified sites in these domains were conserved among species and several variants were found.These results indicate that the conserved domains of duck MEF2A, including the MADS and MEF2 domains, are important for MEF2A transcription factor function.The expression of MEF2A in duck smooth muscle and cardiac muscle suggests that MEF2A plays a role in these two tissues.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, P.R. China.

ABSTRACT
The role of myogenic enhancer transcription factor 2a (MEF2A) in avian muscle during fetal development is unknown. In this work, we cloned the duck MEF2A cDNA sequence (GenBank accession no. HM460752) and examined its developmental expression profiles in cardiac muscle, non-vascular smooth muscle and skeletal muscle. Duck MEF2A cDNA comprised 1479 bp encoding 492 amino acid residues. In silico analysis showed that MEF2A contained MADS (MCM1, AGAMOUS, DEFICIENS and SRF - serum response factor), MEF2 and mitogen-activated protein kinase (MAPK) transcription domains with high homology to related proteins in other species. Modified sites in these domains were conserved among species and several variants were found. Quantitative PCR showed that MEF2A was expressed in all three muscles at each developmental stage examined, with the expression in smooth muscle being higher than in the other muscles. These results indicate that the conserved domains of duck MEF2A, including the MADS and MEF2 domains, are important for MEF2A transcription factor function. The expression of MEF2A in duck smooth muscle and cardiac muscle suggests that MEF2A plays a role in these two tissues.

No MeSH data available.