Limits...
Cloning and expression of embryogenesis-regulating genes in Araucaria angustifolia (Bert.) O. Kuntze (Brazilian Pine).

Schlögl PS, Dos Santos AL, Vieira Ldo N, Floh EI, Guerra MP - Genet. Mol. Biol. (2012)

Bottom Line: Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago.Few studies have covered molecular aspects of embryogenesis in the Brazilian pine, the only economically important native conifer in Brazil.Thus eight embryogenesis-regulating genes, viz., ARGONAUTE 1, CUP-SHAPED COTYLEDON 1, WUSCHEL-related WOX, S-LOCUS LECTIN PROTEIN KINASE, SCARECROW-like, VICILIN 7S, LEAFY COTYLEDON 1, and REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1, were analyzed through semi-quantitative RT-PCR during embryo development and germination.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Departamento de Fitotecnia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.

ABSTRACT
Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago. Apart from morphological and structural differences in embryogenesis and seed origin, a set of embryogenesis-regulating genes and the molecular mechanisms involved in embryo development seem to have been conserved alike in both taxa. Few studies have covered molecular aspects of embryogenesis in the Brazilian pine, the only economically important native conifer in Brazil. Thus eight embryogenesis-regulating genes, viz., ARGONAUTE 1, CUP-SHAPED COTYLEDON 1, WUSCHEL-related WOX, S-LOCUS LECTIN PROTEIN KINASE, SCARECROW-like, VICILIN 7S, LEAFY COTYLEDON 1, and REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1, were analyzed through semi-quantitative RT-PCR during embryo development and germination. All the eight were found to be differentially expressed in the various developmental stages of zygotic embryos, seeds and seedling tissues. To our knowledge, this is the first report on embryogenesis-regulating gene expression in members of the Araucariaceae family, as well as in plants with recalcitrant seeds.

No MeSH data available.


Phylogenetic tree of ARGONAUTE proteins. Multiple alignments were done using ClustalW (version 1.74) software. MEGA software was used in bootstrap analysis and tree construction. Bootstrap percentages are indicated at each fork. Accession numbers: Arabidopsis thaliana - AGO1: NP_849784, AtAGO2: NP_174413, AtAGO3: NP_174414, AtAGO4: NP_565633, AtAGO5: Q9SJK3, AtAGO6: NP180853, AtAGO7: NP_177103, AtAGO8: NP_197602, AtAGO9: CAD66636, AtZLL: NP_199194. Populus trichocarpa - PtZLL: XP_002314663, PtZLL1: XP_002312555, PtAGO1: XP_002329692, PtAGO5: XP_002298162. Brassica napus - BnZLL: ABY52943. Vitis vinifera - VvZLL: XP-002281687, VvAGO5: XP_002271699. Physcomitrella patens - PptAGO1: XP_001757611. Ricinus communis -RcZLL: XP_002517060, RcAGO5: XP_002523757. Araucaria angustifolia - AaAGO: GW924721.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3313508&req=5

f2-gmb-35-1-172: Phylogenetic tree of ARGONAUTE proteins. Multiple alignments were done using ClustalW (version 1.74) software. MEGA software was used in bootstrap analysis and tree construction. Bootstrap percentages are indicated at each fork. Accession numbers: Arabidopsis thaliana - AGO1: NP_849784, AtAGO2: NP_174413, AtAGO3: NP_174414, AtAGO4: NP_565633, AtAGO5: Q9SJK3, AtAGO6: NP180853, AtAGO7: NP_177103, AtAGO8: NP_197602, AtAGO9: CAD66636, AtZLL: NP_199194. Populus trichocarpa - PtZLL: XP_002314663, PtZLL1: XP_002312555, PtAGO1: XP_002329692, PtAGO5: XP_002298162. Brassica napus - BnZLL: ABY52943. Vitis vinifera - VvZLL: XP-002281687, VvAGO5: XP_002271699. Physcomitrella patens - PptAGO1: XP_001757611. Ricinus communis -RcZLL: XP_002517060, RcAGO5: XP_002523757. Araucaria angustifolia - AaAGO: GW924721.

Mentions: AaAGO 1 (GW924721) shared high-deduced amino acid sequence identity with not only putative AGO1 homologues from Populus trichocarpa (97%), but also with AGO1 and ZLL (ZWILLE) proteins from Arabidopsis (91% and 89%, respectively). Sequence alignment with plant AGO proteins was to demonstrate the similarity among sequences from the different taxa (Figure 1). The cloned sequence from AaAGO represented 15% of the full length AGO1 sequence from Arabidopsis, and was shown to share high similarity within a region between the PAZ and PIWI domains (amino acid 693–851). In order to define AaAGO evolutionary relationships, alignments of deduced protein sequences of the AGO protein family from Arabidopsis and other plants were used to construct an unrooted neighbor-joining phylogenetic tree. AaAGO, AGO1, ZLL and AGO5 proteins from various plant species clustered together in Clade I (Vaucheret, 2007), thereby forming a putative cluster of orthologues with 100% bootstrap support, thus implying that AaAGO belongs to the AGO 1 subgroup (Figure 2).


Cloning and expression of embryogenesis-regulating genes in Araucaria angustifolia (Bert.) O. Kuntze (Brazilian Pine).

Schlögl PS, Dos Santos AL, Vieira Ldo N, Floh EI, Guerra MP - Genet. Mol. Biol. (2012)

Phylogenetic tree of ARGONAUTE proteins. Multiple alignments were done using ClustalW (version 1.74) software. MEGA software was used in bootstrap analysis and tree construction. Bootstrap percentages are indicated at each fork. Accession numbers: Arabidopsis thaliana - AGO1: NP_849784, AtAGO2: NP_174413, AtAGO3: NP_174414, AtAGO4: NP_565633, AtAGO5: Q9SJK3, AtAGO6: NP180853, AtAGO7: NP_177103, AtAGO8: NP_197602, AtAGO9: CAD66636, AtZLL: NP_199194. Populus trichocarpa - PtZLL: XP_002314663, PtZLL1: XP_002312555, PtAGO1: XP_002329692, PtAGO5: XP_002298162. Brassica napus - BnZLL: ABY52943. Vitis vinifera - VvZLL: XP-002281687, VvAGO5: XP_002271699. Physcomitrella patens - PptAGO1: XP_001757611. Ricinus communis -RcZLL: XP_002517060, RcAGO5: XP_002523757. Araucaria angustifolia - AaAGO: GW924721.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3313508&req=5

f2-gmb-35-1-172: Phylogenetic tree of ARGONAUTE proteins. Multiple alignments were done using ClustalW (version 1.74) software. MEGA software was used in bootstrap analysis and tree construction. Bootstrap percentages are indicated at each fork. Accession numbers: Arabidopsis thaliana - AGO1: NP_849784, AtAGO2: NP_174413, AtAGO3: NP_174414, AtAGO4: NP_565633, AtAGO5: Q9SJK3, AtAGO6: NP180853, AtAGO7: NP_177103, AtAGO8: NP_197602, AtAGO9: CAD66636, AtZLL: NP_199194. Populus trichocarpa - PtZLL: XP_002314663, PtZLL1: XP_002312555, PtAGO1: XP_002329692, PtAGO5: XP_002298162. Brassica napus - BnZLL: ABY52943. Vitis vinifera - VvZLL: XP-002281687, VvAGO5: XP_002271699. Physcomitrella patens - PptAGO1: XP_001757611. Ricinus communis -RcZLL: XP_002517060, RcAGO5: XP_002523757. Araucaria angustifolia - AaAGO: GW924721.
Mentions: AaAGO 1 (GW924721) shared high-deduced amino acid sequence identity with not only putative AGO1 homologues from Populus trichocarpa (97%), but also with AGO1 and ZLL (ZWILLE) proteins from Arabidopsis (91% and 89%, respectively). Sequence alignment with plant AGO proteins was to demonstrate the similarity among sequences from the different taxa (Figure 1). The cloned sequence from AaAGO represented 15% of the full length AGO1 sequence from Arabidopsis, and was shown to share high similarity within a region between the PAZ and PIWI domains (amino acid 693–851). In order to define AaAGO evolutionary relationships, alignments of deduced protein sequences of the AGO protein family from Arabidopsis and other plants were used to construct an unrooted neighbor-joining phylogenetic tree. AaAGO, AGO1, ZLL and AGO5 proteins from various plant species clustered together in Clade I (Vaucheret, 2007), thereby forming a putative cluster of orthologues with 100% bootstrap support, thus implying that AaAGO belongs to the AGO 1 subgroup (Figure 2).

Bottom Line: Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago.Few studies have covered molecular aspects of embryogenesis in the Brazilian pine, the only economically important native conifer in Brazil.Thus eight embryogenesis-regulating genes, viz., ARGONAUTE 1, CUP-SHAPED COTYLEDON 1, WUSCHEL-related WOX, S-LOCUS LECTIN PROTEIN KINASE, SCARECROW-like, VICILIN 7S, LEAFY COTYLEDON 1, and REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1, were analyzed through semi-quantitative RT-PCR during embryo development and germination.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Departamento de Fitotecnia, Universidade Federal de Santa Catarina, Florianópolis, SC, Brazil.

ABSTRACT
Angiosperm and gymnosperm plants evolved from a common ancestor about 300 million years ago. Apart from morphological and structural differences in embryogenesis and seed origin, a set of embryogenesis-regulating genes and the molecular mechanisms involved in embryo development seem to have been conserved alike in both taxa. Few studies have covered molecular aspects of embryogenesis in the Brazilian pine, the only economically important native conifer in Brazil. Thus eight embryogenesis-regulating genes, viz., ARGONAUTE 1, CUP-SHAPED COTYLEDON 1, WUSCHEL-related WOX, S-LOCUS LECTIN PROTEIN KINASE, SCARECROW-like, VICILIN 7S, LEAFY COTYLEDON 1, and REVERSIBLE GLYCOSYLATED POLYPEPTIDE 1, were analyzed through semi-quantitative RT-PCR during embryo development and germination. All the eight were found to be differentially expressed in the various developmental stages of zygotic embryos, seeds and seedling tissues. To our knowledge, this is the first report on embryogenesis-regulating gene expression in members of the Araucariaceae family, as well as in plants with recalcitrant seeds.

No MeSH data available.