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Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1.

Du YF, Liang L, Shi Y, Long QZ, Zeng J, Wang XY, He DL - Genet. Mol. Biol. (2012)

Bottom Line: In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells.The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis.DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, Shanxi, P.R. China.

ABSTRACT
Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the unfavorable behavior of prostate-cancer cells, thereby implying its potential significance as a promising therapeutic target, with DNMT3A simply in the role of helper.

No MeSH data available.


Related in: MedlinePlus

Results from proliferation (A), migration (B) and invasion (C, D) assays of TSU-PR1 cells after siRNA transfection. Scale bar = 200 μm.
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f3-gmb-35-1-164: Results from proliferation (A), migration (B) and invasion (C, D) assays of TSU-PR1 cells after siRNA transfection. Scale bar = 200 μm.

Mentions: MTT assaying indicated that the knockdown of DNMT3A/B (Group1) or DNMT3B alone (Group 2) significantly inhibited the proliferation of TSU-PR1 cells at 72 h (p < 0.05), whereas silencing DNMT3A (Group 3) alone had no inhibitory effect on the growth of TSU-PR1 cells compared to the negative control (p > 0.05) (Figure 3A).


Multi-target siRNA based on DNMT3A/B homologous conserved region influences cell cycle and apoptosis of human prostate cancer cell line TSU-PR1.

Du YF, Liang L, Shi Y, Long QZ, Zeng J, Wang XY, He DL - Genet. Mol. Biol. (2012)

Results from proliferation (A), migration (B) and invasion (C, D) assays of TSU-PR1 cells after siRNA transfection. Scale bar = 200 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3313507&req=5

f3-gmb-35-1-164: Results from proliferation (A), migration (B) and invasion (C, D) assays of TSU-PR1 cells after siRNA transfection. Scale bar = 200 μm.
Mentions: MTT assaying indicated that the knockdown of DNMT3A/B (Group1) or DNMT3B alone (Group 2) significantly inhibited the proliferation of TSU-PR1 cells at 72 h (p < 0.05), whereas silencing DNMT3A (Group 3) alone had no inhibitory effect on the growth of TSU-PR1 cells compared to the negative control (p > 0.05) (Figure 3A).

Bottom Line: In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells.The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis.DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, First Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, Shanxi, P.R. China.

ABSTRACT
Abnormal genome hypermethylation participates in the tumorigenesis and development of prostate cancer. Prostate cancer cells highly express DNA methyltransferase 3 (DMNT3) family genes, essential for maintaining genome methylation. In the present study, multi-target siRNA, based on the homologous region of the DNMT3 family, was designed for the in vitro investigation of its effects on the proliferation, migration, and invasion of TSU-PR1 prostate cancer cells. The consequential cell-cycle derangement, through DNMT3A/B or only DNMT3B silencing, was partially efficient, without affecting apoptosis. DNMT3A silencing had absolutely no effect on changing TSU-PR1 cell biological behavior. Hence, DNMT3B alone apparently plays a key role in maintaining the unfavorable behavior of prostate-cancer cells, thereby implying its potential significance as a promising therapeutic target, with DNMT3A simply in the role of helper.

No MeSH data available.


Related in: MedlinePlus