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Microbial diversity of soils on the banks of the Solimões and Negro rivers, state of Amazonas, Brazil.

da Costa da Santos EK, Honda RT, Nozawa SR, Ferreira-Nozawa MS - Genet. Mol. Biol. (2012)

Bottom Line: Analysis of bacterial diversity in soils along the banks of the Solimões and Negro rivers, state of Amazonas, Brazil, was by partial sequencing of the genes codifying the rDNA16S region.Diversity of operational taxonomic units (OTU) and of the divergent sequences obtained were applied in comparative analysis of microbiological diversity in the two environments, based on richness estimators and OTU diversity indices.The higher OTU diversity in the Solimões was based on the higher number of parameters that evoke this.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Expressão Gênica, Universidade Nilton Lins, Manaus, AM, Brazil.

ABSTRACT
Analysis of bacterial diversity in soils along the banks of the Solimões and Negro rivers, state of Amazonas, Brazil, was by partial sequencing of the genes codifying the rDNA16S region. Diversity of operational taxonomic units (OTU) and of the divergent sequences obtained were applied in comparative analysis of microbiological diversity in the two environments, based on richness estimators and OTU diversity indices. The higher OTU diversity in the Solimões was based on the higher number of parameters that evoke this. The interaction between the nucleotide sequences of bacteria inhabiting the two riverine environments indicated that the two microrganism communities are similar in composition.

No MeSH data available.


OTU of NMS and SMS in a neighbor-joining tree obtained using the Neighbor-Joining method (bootstrap: 10,000). Distances were calculated using the Maximum-Composite-Likelihood method. Sequence sizes (bp) are shown in brackets.
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f3-gmb-35-1-134: OTU of NMS and SMS in a neighbor-joining tree obtained using the Neighbor-Joining method (bootstrap: 10,000). Distances were calculated using the Maximum-Composite-Likelihood method. Sequence sizes (bp) are shown in brackets.

Mentions: The relationship between OTUs in SMS and NMS was analyzed in combination, using the MEGA 4.1 software, when all 135 OTUs were aligned to obtain a single dendrogram (Figure 3). Notably, some sequences obtained from independent samples collected in either of the two sites were similar (zero distance). Furthermore, there was no formation of distinct clusters.


Microbial diversity of soils on the banks of the Solimões and Negro rivers, state of Amazonas, Brazil.

da Costa da Santos EK, Honda RT, Nozawa SR, Ferreira-Nozawa MS - Genet. Mol. Biol. (2012)

OTU of NMS and SMS in a neighbor-joining tree obtained using the Neighbor-Joining method (bootstrap: 10,000). Distances were calculated using the Maximum-Composite-Likelihood method. Sequence sizes (bp) are shown in brackets.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3313502&req=5

f3-gmb-35-1-134: OTU of NMS and SMS in a neighbor-joining tree obtained using the Neighbor-Joining method (bootstrap: 10,000). Distances were calculated using the Maximum-Composite-Likelihood method. Sequence sizes (bp) are shown in brackets.
Mentions: The relationship between OTUs in SMS and NMS was analyzed in combination, using the MEGA 4.1 software, when all 135 OTUs were aligned to obtain a single dendrogram (Figure 3). Notably, some sequences obtained from independent samples collected in either of the two sites were similar (zero distance). Furthermore, there was no formation of distinct clusters.

Bottom Line: Analysis of bacterial diversity in soils along the banks of the Solimões and Negro rivers, state of Amazonas, Brazil, was by partial sequencing of the genes codifying the rDNA16S region.Diversity of operational taxonomic units (OTU) and of the divergent sequences obtained were applied in comparative analysis of microbiological diversity in the two environments, based on richness estimators and OTU diversity indices.The higher OTU diversity in the Solimões was based on the higher number of parameters that evoke this.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Expressão Gênica, Universidade Nilton Lins, Manaus, AM, Brazil.

ABSTRACT
Analysis of bacterial diversity in soils along the banks of the Solimões and Negro rivers, state of Amazonas, Brazil, was by partial sequencing of the genes codifying the rDNA16S region. Diversity of operational taxonomic units (OTU) and of the divergent sequences obtained were applied in comparative analysis of microbiological diversity in the two environments, based on richness estimators and OTU diversity indices. The higher OTU diversity in the Solimões was based on the higher number of parameters that evoke this. The interaction between the nucleotide sequences of bacteria inhabiting the two riverine environments indicated that the two microrganism communities are similar in composition.

No MeSH data available.