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Genetic architecture of purple pigmentation and tagging of some loci to SSR markers in pearl millet, Pennisetum glaucum (L.) R. Br.

Varalakshmi P, Mohan Dev Tavva SS, Arjuna Rao PV, Subba Rao MV, Hash CT - Genet. Mol. Biol. (2012)

Bottom Line: One of the two loci governing purple seed was associated with the SSR locus Xpsmp2090 in linkage group 1, with a linkage value of 22 cM, while the other locus was associated with the SSR locus Xpsmp2270 in linkage group 6, with a linkage value of 23 cM.The locus for purple pigmentation of the midrib complex was either responsible for pigmentation of the panicle in a pleiotropic manner or was linked to it very closely and associated with the SSR locus Xpsmp2086 in linkage group 4, with a suggestive linkage value of 21 cM.These findings suggest that the locus for pigmentation of the midrib complex might regulate the basic steps in anthocyanin pigment development by acting as a structural gene while other loci regulate the formation of color in specific plant parts.

View Article: PubMed Central - PubMed

Affiliation: Plant Cytogenetics and Biotechnology Laboratory, Botany Department, Andhra University, Visakhapatnam, India.

ABSTRACT
This report describes the construction of integrated genetic maps in pearl millet involving certain purple phenotype and simple sequence repeat (SSR) markers. These maps provide a direct means of implementing DNA marker-assisted selection and of facilitating "map-based cloning" for engineering novel traits. The purple pigmentation of leaf sheath, midrib and leaf margin was inherited together 'en bloc' under the control of a single dominant locus (the 'midrib complex') and was inseparably associated with the locus governing the purple coloration of the internode. The purple panicle was caused by a single dominant locus. Each of the three characters (purple lamina, purple stigma and purple seed) was governed by two complementary loci. One of the two loci governing purple seed was associated with the SSR locus Xpsmp2090 in linkage group 1, with a linkage value of 22 cM, while the other locus was associated with the SSR locus Xpsmp2270 in linkage group 6, with a linkage value of 23 cM. The locus for purple pigmentation of the midrib complex was either responsible for pigmentation of the panicle in a pleiotropic manner or was linked to it very closely and associated with the SSR locus Xpsmp2086 in linkage group 4, with a suggestive linkage value of 21 cM. A dominant allele at this locus seems to be a prerequisite for the development of purple pigmentation in the lamina, stigma and seed. These findings suggest that the locus for pigmentation of the midrib complex might regulate the basic steps in anthocyanin pigment development by acting as a structural gene while other loci regulate the formation of color in specific plant parts.

No MeSH data available.


Related in: MedlinePlus

Purple and non-purple phenotypes of pearl millet. a. Plant of purple parental accession. b. Plant of green dwarf (d2) parental accession. c. Purple parent showing brachytic nature, pigmentation of leaf sheath (double arrow) and initiation of purple pigmentation in the lamina (single arrow). d. Leaf of purple parent showing initiation of purple pigmentation at the base of the lamina. e. Leaf of purple parent showing pigmentation of the entire lamina. f. Purple parent showing purple internode (double arrow) and green node (single arrow). g. F2 segregant with green lamina and purple midrib complex (purple midrib, purple sheath and purple lamina). h. Panicle of purple parent showing development of pigmentation on the exposed part. i. Purple panicle with purple stigmas at the time of emergence. j. Green panicle with white stigmas. k. F2 segregant with purple panicle and green stigmas at the initiation of anthesis. l. Grey (A) and purple (B) seeds from the parental accessions.
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f1-gmb-35-1-106: Purple and non-purple phenotypes of pearl millet. a. Plant of purple parental accession. b. Plant of green dwarf (d2) parental accession. c. Purple parent showing brachytic nature, pigmentation of leaf sheath (double arrow) and initiation of purple pigmentation in the lamina (single arrow). d. Leaf of purple parent showing initiation of purple pigmentation at the base of the lamina. e. Leaf of purple parent showing pigmentation of the entire lamina. f. Purple parent showing purple internode (double arrow) and green node (single arrow). g. F2 segregant with green lamina and purple midrib complex (purple midrib, purple sheath and purple lamina). h. Panicle of purple parent showing development of pigmentation on the exposed part. i. Purple panicle with purple stigmas at the time of emergence. j. Green panicle with white stigmas. k. F2 segregant with purple panicle and green stigmas at the initiation of anthesis. l. Grey (A) and purple (B) seeds from the parental accessions.

Mentions: The inheritance patterns of purple pigmentation of the leaf, panicle, stigma and seed coat were studied individually and in combinations using reciprocal crosses between plants of the two parental lines, viz., green dwarf (d2) and purple (IP3128; Figure 1a,b). A common feature of inheritance noted for all of these phenotypic markers was the lack of reciprocal differences as judged from the F1, F2 and testcross progeny phenotypes.


Genetic architecture of purple pigmentation and tagging of some loci to SSR markers in pearl millet, Pennisetum glaucum (L.) R. Br.

Varalakshmi P, Mohan Dev Tavva SS, Arjuna Rao PV, Subba Rao MV, Hash CT - Genet. Mol. Biol. (2012)

Purple and non-purple phenotypes of pearl millet. a. Plant of purple parental accession. b. Plant of green dwarf (d2) parental accession. c. Purple parent showing brachytic nature, pigmentation of leaf sheath (double arrow) and initiation of purple pigmentation in the lamina (single arrow). d. Leaf of purple parent showing initiation of purple pigmentation at the base of the lamina. e. Leaf of purple parent showing pigmentation of the entire lamina. f. Purple parent showing purple internode (double arrow) and green node (single arrow). g. F2 segregant with green lamina and purple midrib complex (purple midrib, purple sheath and purple lamina). h. Panicle of purple parent showing development of pigmentation on the exposed part. i. Purple panicle with purple stigmas at the time of emergence. j. Green panicle with white stigmas. k. F2 segregant with purple panicle and green stigmas at the initiation of anthesis. l. Grey (A) and purple (B) seeds from the parental accessions.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3313498&req=5

f1-gmb-35-1-106: Purple and non-purple phenotypes of pearl millet. a. Plant of purple parental accession. b. Plant of green dwarf (d2) parental accession. c. Purple parent showing brachytic nature, pigmentation of leaf sheath (double arrow) and initiation of purple pigmentation in the lamina (single arrow). d. Leaf of purple parent showing initiation of purple pigmentation at the base of the lamina. e. Leaf of purple parent showing pigmentation of the entire lamina. f. Purple parent showing purple internode (double arrow) and green node (single arrow). g. F2 segregant with green lamina and purple midrib complex (purple midrib, purple sheath and purple lamina). h. Panicle of purple parent showing development of pigmentation on the exposed part. i. Purple panicle with purple stigmas at the time of emergence. j. Green panicle with white stigmas. k. F2 segregant with purple panicle and green stigmas at the initiation of anthesis. l. Grey (A) and purple (B) seeds from the parental accessions.
Mentions: The inheritance patterns of purple pigmentation of the leaf, panicle, stigma and seed coat were studied individually and in combinations using reciprocal crosses between plants of the two parental lines, viz., green dwarf (d2) and purple (IP3128; Figure 1a,b). A common feature of inheritance noted for all of these phenotypic markers was the lack of reciprocal differences as judged from the F1, F2 and testcross progeny phenotypes.

Bottom Line: One of the two loci governing purple seed was associated with the SSR locus Xpsmp2090 in linkage group 1, with a linkage value of 22 cM, while the other locus was associated with the SSR locus Xpsmp2270 in linkage group 6, with a linkage value of 23 cM.The locus for purple pigmentation of the midrib complex was either responsible for pigmentation of the panicle in a pleiotropic manner or was linked to it very closely and associated with the SSR locus Xpsmp2086 in linkage group 4, with a suggestive linkage value of 21 cM.These findings suggest that the locus for pigmentation of the midrib complex might regulate the basic steps in anthocyanin pigment development by acting as a structural gene while other loci regulate the formation of color in specific plant parts.

View Article: PubMed Central - PubMed

Affiliation: Plant Cytogenetics and Biotechnology Laboratory, Botany Department, Andhra University, Visakhapatnam, India.

ABSTRACT
This report describes the construction of integrated genetic maps in pearl millet involving certain purple phenotype and simple sequence repeat (SSR) markers. These maps provide a direct means of implementing DNA marker-assisted selection and of facilitating "map-based cloning" for engineering novel traits. The purple pigmentation of leaf sheath, midrib and leaf margin was inherited together 'en bloc' under the control of a single dominant locus (the 'midrib complex') and was inseparably associated with the locus governing the purple coloration of the internode. The purple panicle was caused by a single dominant locus. Each of the three characters (purple lamina, purple stigma and purple seed) was governed by two complementary loci. One of the two loci governing purple seed was associated with the SSR locus Xpsmp2090 in linkage group 1, with a linkage value of 22 cM, while the other locus was associated with the SSR locus Xpsmp2270 in linkage group 6, with a linkage value of 23 cM. The locus for purple pigmentation of the midrib complex was either responsible for pigmentation of the panicle in a pleiotropic manner or was linked to it very closely and associated with the SSR locus Xpsmp2086 in linkage group 4, with a suggestive linkage value of 21 cM. A dominant allele at this locus seems to be a prerequisite for the development of purple pigmentation in the lamina, stigma and seed. These findings suggest that the locus for pigmentation of the midrib complex might regulate the basic steps in anthocyanin pigment development by acting as a structural gene while other loci regulate the formation of color in specific plant parts.

No MeSH data available.


Related in: MedlinePlus