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Involvement of the ubiquitin-proteasome system in the formation of experimental postsurgical peritoneal adhesions.

Di Filippo C, Petronella P, Freda F, Scorzelli M, Ferretti M, Canonico S, Rossi F, D'Amico M - Mediators Inflamm. (2012)

Bottom Line: We assayed the levels of UPS within the adhered tissue along with the development of peritoneal adhesions and used the specific UPS inhibitor bortezomib in order to assess the effect of the UPS blockade on the peritoneal adhesions.In the adhered tissue an increased values of ubiquitin and the 20S proteasome subunit, NFkB, IL-6, TNF-α and decreased values of IkB-beta were found.In contrast, bortezomib-treated rats showed a decreased number of peritoneal adhesions, decreased values of ubiquitin and the 20S proteasome, NFkB, IL-6, TNF-α, and increased levels of IkB-beta in the adhered peritoneal tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Second University of Naples, Naples, Italy.

ABSTRACT
We investigated the Ubiquitin-Proteasome System (UPS), major nonlysosomal intracellular protein degradation system, in the genesis of experimental postsurgical peritoneal adhesions. We assayed the levels of UPS within the adhered tissue along with the development of peritoneal adhesions and used the specific UPS inhibitor bortezomib in order to assess the effect of the UPS blockade on the peritoneal adhesions. We found a number of severe postsurgical peritoneal adhesions at day 5 after surgery increasing until day 10. In the adhered tissue an increased values of ubiquitin and the 20S proteasome subunit, NFkB, IL-6, TNF-α and decreased values of IkB-beta were found. In contrast, bortezomib-treated rats showed a decreased number of peritoneal adhesions, decreased values of ubiquitin and the 20S proteasome, NFkB, IL-6, TNF-α, and increased levels of IkB-beta in the adhered peritoneal tissue. The UPS system, therefore, is primarily involved in the formation of post-surgical peritoneal adhesions in rats.

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Correlation between the adhesion score and the local levels of ubiquitin and protesome 20S 10 days after the surgery.
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fig4: Correlation between the adhesion score and the local levels of ubiquitin and protesome 20S 10 days after the surgery.

Mentions: The qualitative evaluation by the peritoneal adhesions score indicates a substantial decrease of adhesions in the group of rats treated with bortezomib (e.g., 4 rats were scored 6 and 2 rats were scored 5) after 10 days of surgery if compared with the control group same day (Figure 2). This was accompinied by a significant reduction of the levels of both ubiquitin and proteasome 20S (P < 0.01 versus day 0) in the peritoneal tissue 5 days after surgery. The reduction was found to be significant even 10 days after surgical procedure (P < 0.01 versus day 0) (Figure 3). At this time point there was a significant correlation between the levels of ubiquitin/proteasome 20S and the adhesion score assigned to the rats after bortezomib treatment (Figure 4).


Involvement of the ubiquitin-proteasome system in the formation of experimental postsurgical peritoneal adhesions.

Di Filippo C, Petronella P, Freda F, Scorzelli M, Ferretti M, Canonico S, Rossi F, D'Amico M - Mediators Inflamm. (2012)

Correlation between the adhesion score and the local levels of ubiquitin and protesome 20S 10 days after the surgery.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3306991&req=5

fig4: Correlation between the adhesion score and the local levels of ubiquitin and protesome 20S 10 days after the surgery.
Mentions: The qualitative evaluation by the peritoneal adhesions score indicates a substantial decrease of adhesions in the group of rats treated with bortezomib (e.g., 4 rats were scored 6 and 2 rats were scored 5) after 10 days of surgery if compared with the control group same day (Figure 2). This was accompinied by a significant reduction of the levels of both ubiquitin and proteasome 20S (P < 0.01 versus day 0) in the peritoneal tissue 5 days after surgery. The reduction was found to be significant even 10 days after surgical procedure (P < 0.01 versus day 0) (Figure 3). At this time point there was a significant correlation between the levels of ubiquitin/proteasome 20S and the adhesion score assigned to the rats after bortezomib treatment (Figure 4).

Bottom Line: We assayed the levels of UPS within the adhered tissue along with the development of peritoneal adhesions and used the specific UPS inhibitor bortezomib in order to assess the effect of the UPS blockade on the peritoneal adhesions.In the adhered tissue an increased values of ubiquitin and the 20S proteasome subunit, NFkB, IL-6, TNF-α and decreased values of IkB-beta were found.In contrast, bortezomib-treated rats showed a decreased number of peritoneal adhesions, decreased values of ubiquitin and the 20S proteasome, NFkB, IL-6, TNF-α, and increased levels of IkB-beta in the adhered peritoneal tissue.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, Second University of Naples, Naples, Italy.

ABSTRACT
We investigated the Ubiquitin-Proteasome System (UPS), major nonlysosomal intracellular protein degradation system, in the genesis of experimental postsurgical peritoneal adhesions. We assayed the levels of UPS within the adhered tissue along with the development of peritoneal adhesions and used the specific UPS inhibitor bortezomib in order to assess the effect of the UPS blockade on the peritoneal adhesions. We found a number of severe postsurgical peritoneal adhesions at day 5 after surgery increasing until day 10. In the adhered tissue an increased values of ubiquitin and the 20S proteasome subunit, NFkB, IL-6, TNF-α and decreased values of IkB-beta were found. In contrast, bortezomib-treated rats showed a decreased number of peritoneal adhesions, decreased values of ubiquitin and the 20S proteasome, NFkB, IL-6, TNF-α, and increased levels of IkB-beta in the adhered peritoneal tissue. The UPS system, therefore, is primarily involved in the formation of post-surgical peritoneal adhesions in rats.

Show MeSH
Related in: MedlinePlus