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Proteomic profiling of thyroid papillary carcinoma.

Ban Y, Yamamoto G, Takada M, Hayashi S, Ban Y, Shimizu K, Akasu H, Igarashi T, Bando Y, Tachikawa T, Hirano T - J Thyroid Res (2012)

Bottom Line: Reproducibility was confirmed with several known markers, but the one of two new candidate markers such as MLEC did not show large variations in expression levels.Furthermore, IHC confirmed the overexpression of both those markers in PTCs compared with normal surrounding tissues.Our protein data suggest that NPNT and MLEC could be a characteristic marker for PTC.

View Article: PubMed Central - PubMed

Affiliation: Division of Diabetes, Metabolism and Endocrinology, Department of Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8666, Japan.

ABSTRACT
Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy. We performed shotgun liquid chromatography (LC)/tandem mass spectrometry (MS/MS) analysis on pooled protein extracts from patients with PTC and compared the results with those from normal thyroid tissue validated by real-time (RT) PCR and immunohistochemistry (IHC). We detected 524 types of protein in PTC and 432 in normal thyroid gland. Among these proteins, 145 were specific to PTC and 53 were specific to normal thyroid gland. We have also identified two important new markers, nephronectin (NPNT) and malectin (MLEC). Reproducibility was confirmed with several known markers, but the one of two new candidate markers such as MLEC did not show large variations in expression levels. Furthermore, IHC confirmed the overexpression of both those markers in PTCs compared with normal surrounding tissues. Our protein data suggest that NPNT and MLEC could be a characteristic marker for PTC.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemical localization of NPNT and MLEC in normal thyroid tissue and PTC. Only weak staining of NPNT and MLEC were observed in follicular epithelium of normal thyroid tissue (surrounding tumor). In PTC, strong staining was seen with both antibodies (Normal: normal thyroid tissue, PTC: Papillary thyroid carcinoma).
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fig5: Immunohistochemical localization of NPNT and MLEC in normal thyroid tissue and PTC. Only weak staining of NPNT and MLEC were observed in follicular epithelium of normal thyroid tissue (surrounding tumor). In PTC, strong staining was seen with both antibodies (Normal: normal thyroid tissue, PTC: Papillary thyroid carcinoma).

Mentions: The distribution of NPNT and MLEC protein expression in normal thyroid tissue and PTC was examined by immunohistochemical analysis. Few positive reactions for NPNT and MLEC were observed in the follicular epithelium (surrounding tumor); on the other hand, carcinoma cells were strongly stained (Figure 5). Particularly for MLEC, no significant differences in gene expression were observed between normal thyroid tissue and PTC; nevertheless, the protein localization was clearly different.


Proteomic profiling of thyroid papillary carcinoma.

Ban Y, Yamamoto G, Takada M, Hayashi S, Ban Y, Shimizu K, Akasu H, Igarashi T, Bando Y, Tachikawa T, Hirano T - J Thyroid Res (2012)

Immunohistochemical localization of NPNT and MLEC in normal thyroid tissue and PTC. Only weak staining of NPNT and MLEC were observed in follicular epithelium of normal thyroid tissue (surrounding tumor). In PTC, strong staining was seen with both antibodies (Normal: normal thyroid tissue, PTC: Papillary thyroid carcinoma).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3306988&req=5

fig5: Immunohistochemical localization of NPNT and MLEC in normal thyroid tissue and PTC. Only weak staining of NPNT and MLEC were observed in follicular epithelium of normal thyroid tissue (surrounding tumor). In PTC, strong staining was seen with both antibodies (Normal: normal thyroid tissue, PTC: Papillary thyroid carcinoma).
Mentions: The distribution of NPNT and MLEC protein expression in normal thyroid tissue and PTC was examined by immunohistochemical analysis. Few positive reactions for NPNT and MLEC were observed in the follicular epithelium (surrounding tumor); on the other hand, carcinoma cells were strongly stained (Figure 5). Particularly for MLEC, no significant differences in gene expression were observed between normal thyroid tissue and PTC; nevertheless, the protein localization was clearly different.

Bottom Line: Reproducibility was confirmed with several known markers, but the one of two new candidate markers such as MLEC did not show large variations in expression levels.Furthermore, IHC confirmed the overexpression of both those markers in PTCs compared with normal surrounding tissues.Our protein data suggest that NPNT and MLEC could be a characteristic marker for PTC.

View Article: PubMed Central - PubMed

Affiliation: Division of Diabetes, Metabolism and Endocrinology, Department of Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8666, Japan.

ABSTRACT
Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy. We performed shotgun liquid chromatography (LC)/tandem mass spectrometry (MS/MS) analysis on pooled protein extracts from patients with PTC and compared the results with those from normal thyroid tissue validated by real-time (RT) PCR and immunohistochemistry (IHC). We detected 524 types of protein in PTC and 432 in normal thyroid gland. Among these proteins, 145 were specific to PTC and 53 were specific to normal thyroid gland. We have also identified two important new markers, nephronectin (NPNT) and malectin (MLEC). Reproducibility was confirmed with several known markers, but the one of two new candidate markers such as MLEC did not show large variations in expression levels. Furthermore, IHC confirmed the overexpression of both those markers in PTCs compared with normal surrounding tissues. Our protein data suggest that NPNT and MLEC could be a characteristic marker for PTC.

No MeSH data available.


Related in: MedlinePlus