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Microarray Analyses of Genes Differentially Expressed by Diet (Black Beans and Soy Flour) during Azoxymethane-Induced Colon Carcinogenesis in Rats.

Rondini EA, Bennink MR - J Nutr Metab (2012)

Bottom Line: AOM treatment induced a number of genes involved in immunity, including several MHC II-associated antigens and innate defense genes (RatNP-3, Lyz2, Pla2g2a).Genes involved in the extracellular matrix (Col1a1, Fn1) and innate immunity (RatNP-3, Pla2g2a) were induced by AOM in all diets, but to a lower extent in bean-fed animals.This profile suggests beans inhibit colon carcinogenesis by modulating cellular kinetics and reducing inflammation, potentially by preserving mucosal barrier function.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science and Human Nutrition, 106 GM Trout Building, Michigan State University, East Lansing, MI 48824, USA.

ABSTRACT
We previously demonstrated that black bean (BB) and soy flour (SF)-based diets inhibit azoxymethane (AOM)-induced colon cancer. The objective of this study was to identify genes altered by carcinogen treatment in normal-appearing colonic mucosa and those attenuated by bean feeding. Ninety-five male F344 rats were fed control (AIN) diets upon arrival. At 4 and 5 weeks, rats were injected with AOM (15 mg/kg) or saline and one week later administered an AIN, BB-, or SF-based diet. Rats were sacrificed after 31 weeks, and microarrays were conducted on RNA isolated from the distal colonic mucosa. AOM treatment induced a number of genes involved in immunity, including several MHC II-associated antigens and innate defense genes (RatNP-3, Lyz2, Pla2g2a). BB- and SF-fed rats exhibited a higher expression of genes involved in energy metabolism and water and sodium absorption and lower expression of innate (RatNP-3, Pla2g2a, Tlr4, Dmbt1) and cell cycle-associated (Cdc2, Ccnb1, Top2a) genes. Genes involved in the extracellular matrix (Col1a1, Fn1) and innate immunity (RatNP-3, Pla2g2a) were induced by AOM in all diets, but to a lower extent in bean-fed animals. This profile suggests beans inhibit colon carcinogenesis by modulating cellular kinetics and reducing inflammation, potentially by preserving mucosal barrier function.

No MeSH data available.


Related in: MedlinePlus

Functional classification of genes significantly altered by carcinogen (AOM) and by dietary treatment in the distal colon mucosa of rats detected by microarrays. A total of 155 genes were altered by carcinogen (AOM) and 257 by dietary treatment (AIN versus BB versus SF, P < 0.05).
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Related In: Results  -  Collection


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fig2: Functional classification of genes significantly altered by carcinogen (AOM) and by dietary treatment in the distal colon mucosa of rats detected by microarrays. A total of 155 genes were altered by carcinogen (AOM) and 257 by dietary treatment (AIN versus BB versus SF, P < 0.05).

Mentions: Among the 8799 genes and ESTs present on the rat genome UG34A array, a total of 155 transcripts were significantly affected by injection regime (AOM versus saline), 257 by dietary treatment (AIN, BB, SF), and 5 were affected by both (P < 0.05). Transcripts differentially expressed by either carcinogen or diet were broadly classified into one of twelve functional categories and results are depicted in Figure 2.


Microarray Analyses of Genes Differentially Expressed by Diet (Black Beans and Soy Flour) during Azoxymethane-Induced Colon Carcinogenesis in Rats.

Rondini EA, Bennink MR - J Nutr Metab (2012)

Functional classification of genes significantly altered by carcinogen (AOM) and by dietary treatment in the distal colon mucosa of rats detected by microarrays. A total of 155 genes were altered by carcinogen (AOM) and 257 by dietary treatment (AIN versus BB versus SF, P < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3306975&req=5

fig2: Functional classification of genes significantly altered by carcinogen (AOM) and by dietary treatment in the distal colon mucosa of rats detected by microarrays. A total of 155 genes were altered by carcinogen (AOM) and 257 by dietary treatment (AIN versus BB versus SF, P < 0.05).
Mentions: Among the 8799 genes and ESTs present on the rat genome UG34A array, a total of 155 transcripts were significantly affected by injection regime (AOM versus saline), 257 by dietary treatment (AIN, BB, SF), and 5 were affected by both (P < 0.05). Transcripts differentially expressed by either carcinogen or diet were broadly classified into one of twelve functional categories and results are depicted in Figure 2.

Bottom Line: AOM treatment induced a number of genes involved in immunity, including several MHC II-associated antigens and innate defense genes (RatNP-3, Lyz2, Pla2g2a).Genes involved in the extracellular matrix (Col1a1, Fn1) and innate immunity (RatNP-3, Pla2g2a) were induced by AOM in all diets, but to a lower extent in bean-fed animals.This profile suggests beans inhibit colon carcinogenesis by modulating cellular kinetics and reducing inflammation, potentially by preserving mucosal barrier function.

View Article: PubMed Central - PubMed

Affiliation: Department of Food Science and Human Nutrition, 106 GM Trout Building, Michigan State University, East Lansing, MI 48824, USA.

ABSTRACT
We previously demonstrated that black bean (BB) and soy flour (SF)-based diets inhibit azoxymethane (AOM)-induced colon cancer. The objective of this study was to identify genes altered by carcinogen treatment in normal-appearing colonic mucosa and those attenuated by bean feeding. Ninety-five male F344 rats were fed control (AIN) diets upon arrival. At 4 and 5 weeks, rats were injected with AOM (15 mg/kg) or saline and one week later administered an AIN, BB-, or SF-based diet. Rats were sacrificed after 31 weeks, and microarrays were conducted on RNA isolated from the distal colonic mucosa. AOM treatment induced a number of genes involved in immunity, including several MHC II-associated antigens and innate defense genes (RatNP-3, Lyz2, Pla2g2a). BB- and SF-fed rats exhibited a higher expression of genes involved in energy metabolism and water and sodium absorption and lower expression of innate (RatNP-3, Pla2g2a, Tlr4, Dmbt1) and cell cycle-associated (Cdc2, Ccnb1, Top2a) genes. Genes involved in the extracellular matrix (Col1a1, Fn1) and innate immunity (RatNP-3, Pla2g2a) were induced by AOM in all diets, but to a lower extent in bean-fed animals. This profile suggests beans inhibit colon carcinogenesis by modulating cellular kinetics and reducing inflammation, potentially by preserving mucosal barrier function.

No MeSH data available.


Related in: MedlinePlus