Limits...
Hydatid Cyst Protoscolices Induce Cell Death in WEHI-164 Fibrosarcoma Cells and Inhibit the Proliferation of Baby Hamster Kidney Fibroblasts In Vitro.

Yousofi Darani H, Soozangar N, Khorami S, Taji F, Yousofi M, Shirzad H - J Parasitol Res (2012)

Bottom Line: Both in vitro and in vivo models have demonstrated that some parasites can interfere with tumor cell growth.After 48 h, lactate dehydrogenase (LDH) and cell counts were assayed for both treated cells and control groups.Following treatment with hydatid cyst protoscolices, cell proliferation of both cell types was inhibited, and lysis of fibrosarcoma cells increased.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Cell and Molecular Research Center, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.

ABSTRACT
Both in vitro and in vivo models have demonstrated that some parasites can interfere with tumor cell growth. The present study investigates the anticancer activity of hydatid cyst protoscolices on WEHI-164 fibrosarcoma cells and baby hamster kidney (BHK) fibroblast cells in vitro. Those above two cell types were treated with live hydatid cyst protoscolices or left untreated for control groups. After 48 h, lactate dehydrogenase (LDH) and cell counts were assayed for both treated cells and control groups. Following treatment with hydatid cyst protoscolices, cell proliferation of both cell types was inhibited, and lysis of fibrosarcoma cells increased. Based on these results, it appears that hydatid cyst protoscolices have strong anticancer activity, and additional studies are needed to further clarify the mechanisms of this activity.

No MeSH data available.


Related in: MedlinePlus

The number of viable or dead cells (×105) in flasks of BHK fibroblast cell 48 h after treatment with 100 hydatid cyst protoscolices compared to control cells (mean of triplicate performance).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3306915&req=5

fig3: The number of viable or dead cells (×105) in flasks of BHK fibroblast cell 48 h after treatment with 100 hydatid cyst protoscolices compared to control cells (mean of triplicate performance).

Mentions: In similar experiments, the effects of hydatid cyst protoscolices on the proliferation and viability of BHK fibroblast cells were evaluated in comparison with untreated BHK cells (control group). A significant difference between the number of viable cells detected in treated and control groups was observed. However, the difference in the number of dead cells associated with each group was not significant (Table 2 and Figure 3).


Hydatid Cyst Protoscolices Induce Cell Death in WEHI-164 Fibrosarcoma Cells and Inhibit the Proliferation of Baby Hamster Kidney Fibroblasts In Vitro.

Yousofi Darani H, Soozangar N, Khorami S, Taji F, Yousofi M, Shirzad H - J Parasitol Res (2012)

The number of viable or dead cells (×105) in flasks of BHK fibroblast cell 48 h after treatment with 100 hydatid cyst protoscolices compared to control cells (mean of triplicate performance).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3306915&req=5

fig3: The number of viable or dead cells (×105) in flasks of BHK fibroblast cell 48 h after treatment with 100 hydatid cyst protoscolices compared to control cells (mean of triplicate performance).
Mentions: In similar experiments, the effects of hydatid cyst protoscolices on the proliferation and viability of BHK fibroblast cells were evaluated in comparison with untreated BHK cells (control group). A significant difference between the number of viable cells detected in treated and control groups was observed. However, the difference in the number of dead cells associated with each group was not significant (Table 2 and Figure 3).

Bottom Line: Both in vitro and in vivo models have demonstrated that some parasites can interfere with tumor cell growth.After 48 h, lactate dehydrogenase (LDH) and cell counts were assayed for both treated cells and control groups.Following treatment with hydatid cyst protoscolices, cell proliferation of both cell types was inhibited, and lysis of fibrosarcoma cells increased.

View Article: PubMed Central - PubMed

Affiliation: Department of Parasitology, Cell and Molecular Research Center, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.

ABSTRACT
Both in vitro and in vivo models have demonstrated that some parasites can interfere with tumor cell growth. The present study investigates the anticancer activity of hydatid cyst protoscolices on WEHI-164 fibrosarcoma cells and baby hamster kidney (BHK) fibroblast cells in vitro. Those above two cell types were treated with live hydatid cyst protoscolices or left untreated for control groups. After 48 h, lactate dehydrogenase (LDH) and cell counts were assayed for both treated cells and control groups. Following treatment with hydatid cyst protoscolices, cell proliferation of both cell types was inhibited, and lysis of fibrosarcoma cells increased. Based on these results, it appears that hydatid cyst protoscolices have strong anticancer activity, and additional studies are needed to further clarify the mechanisms of this activity.

No MeSH data available.


Related in: MedlinePlus