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Chemokine and free fatty acid levels in insulin-resistant state of successful pregnancy: a preliminary observation.

Naruse K, Noguchi T, Sado T, Tsunemi T, Shigetomi H, Kanayama S, Akasaka J, Koike N, Oi H, Kobayashi H - Mediators Inflamm. (2012)

Bottom Line: FFA/albumin, MCP-1, and HMW adiponectin concentrations were significantly higher in PIH patients than in women with normal pregnancies.The 3 measurements of FFA/albumin showed a significant increase through the course of uncomplicated pregnancies.In contrast, MCP-1 and HMW adiponectin were significantly decreased during the course of pregnancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics & Gynecology, Nara Medical University, Kashihara City, Nara, Japan. naruse@naramed-u.ac.jp

ABSTRACT
Increased insulin resistance and inflammatory action are observed in pregnancy-induced hypertension (PIH), but similar insulin resistance is observed also in successful pregnancy. To estimate insulin resistance and inflammatory activity in normal pregnancy and PIH, serum concentrations of free fatty acids (FFA; corrected with albumin to estimate unbound FFA), monocyte chemoattractant protein (MCP)-1, and high-molecular weight (HMW) adiponectin were measured in severe PIH patients with a BMI less than 25 kg/m(2) and were measured 3 times during the course of pregnancy in women with normal pregnancies. FFA/albumin, MCP-1, and HMW adiponectin concentrations were significantly higher in PIH patients than in women with normal pregnancies. The 3 measurements of FFA/albumin showed a significant increase through the course of uncomplicated pregnancies. In contrast, MCP-1 and HMW adiponectin were significantly decreased during the course of pregnancy. These results suggest that the reduced MCP-1 concentration in normal pregnancy may be a pathway to inhibit the induction of pathological features from physiological insulin resistance and homeostatic inflammation.

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(a) Serum concentrations of FFA (μM)/albumin (g/dL), (b) MCP-1 (pg/mL), and (c) high-molecular weight (HMW) adiponectin (μg/mL) during normal pregnancy and PIH (for reference; see Table 1). In normal pregnancy, samplings were performed 3 times in each subject (see Table 2) and analyzed in pairs. Data are shown as the 90th, 75th, 50th, 25th, and 10th percentile of each measurement group. *P < 0.05 in repeated measures of ANOVA with the post hoc test.
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fig1: (a) Serum concentrations of FFA (μM)/albumin (g/dL), (b) MCP-1 (pg/mL), and (c) high-molecular weight (HMW) adiponectin (μg/mL) during normal pregnancy and PIH (for reference; see Table 1). In normal pregnancy, samplings were performed 3 times in each subject (see Table 2) and analyzed in pairs. Data are shown as the 90th, 75th, 50th, 25th, and 10th percentile of each measurement group. *P < 0.05 in repeated measures of ANOVA with the post hoc test.

Mentions: Subject characteristics are shown in Table 2. FFA concentrations were not significantly altered in normal pregnant women over the course of 3 measurements: 1st screening, 97.47 ± 13.05 μM; 2nd, 110.89 ± 12.78; 3rd, 120.85 ± 12.24. However, after the albumin correction (1st screening, 3.15 ± 0.04 g/dL; 2nd, 2.57 ± 0.03; 3rd, 2.52 ± 0.03) to estimate the alteration of unbound FFA, the value (FFA [μM]/albumin [g/dL]) was significantly increased throughout the course of pregnancy: 1st screening, 31.38 ± 4.29; 2nd, 42.51 ± 4.82; 3rd, 48.45 ± 5.10; P = 0.0048 (Figure 1). In contrast, MCP-1 concentrations decreased significantly during the course of pregnancy: 1st screening, 154.36 ± 20.27 pg/mL; 2nd, 110.56 ± 33.44; 3rd, 108.78 ± 28.17; P < 0.0001 (Figure 1). HMW adiponectin was also significantly decreased during the course of pregnancy: 1st screening, 3.48 ± 0.30 μg/mL; 2nd, 2.88 ± 0.27; 3rd, 2.86 ± 0.25; P = 0.0001 (Figure 1).


Chemokine and free fatty acid levels in insulin-resistant state of successful pregnancy: a preliminary observation.

Naruse K, Noguchi T, Sado T, Tsunemi T, Shigetomi H, Kanayama S, Akasaka J, Koike N, Oi H, Kobayashi H - Mediators Inflamm. (2012)

(a) Serum concentrations of FFA (μM)/albumin (g/dL), (b) MCP-1 (pg/mL), and (c) high-molecular weight (HMW) adiponectin (μg/mL) during normal pregnancy and PIH (for reference; see Table 1). In normal pregnancy, samplings were performed 3 times in each subject (see Table 2) and analyzed in pairs. Data are shown as the 90th, 75th, 50th, 25th, and 10th percentile of each measurement group. *P < 0.05 in repeated measures of ANOVA with the post hoc test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3306909&req=5

fig1: (a) Serum concentrations of FFA (μM)/albumin (g/dL), (b) MCP-1 (pg/mL), and (c) high-molecular weight (HMW) adiponectin (μg/mL) during normal pregnancy and PIH (for reference; see Table 1). In normal pregnancy, samplings were performed 3 times in each subject (see Table 2) and analyzed in pairs. Data are shown as the 90th, 75th, 50th, 25th, and 10th percentile of each measurement group. *P < 0.05 in repeated measures of ANOVA with the post hoc test.
Mentions: Subject characteristics are shown in Table 2. FFA concentrations were not significantly altered in normal pregnant women over the course of 3 measurements: 1st screening, 97.47 ± 13.05 μM; 2nd, 110.89 ± 12.78; 3rd, 120.85 ± 12.24. However, after the albumin correction (1st screening, 3.15 ± 0.04 g/dL; 2nd, 2.57 ± 0.03; 3rd, 2.52 ± 0.03) to estimate the alteration of unbound FFA, the value (FFA [μM]/albumin [g/dL]) was significantly increased throughout the course of pregnancy: 1st screening, 31.38 ± 4.29; 2nd, 42.51 ± 4.82; 3rd, 48.45 ± 5.10; P = 0.0048 (Figure 1). In contrast, MCP-1 concentrations decreased significantly during the course of pregnancy: 1st screening, 154.36 ± 20.27 pg/mL; 2nd, 110.56 ± 33.44; 3rd, 108.78 ± 28.17; P < 0.0001 (Figure 1). HMW adiponectin was also significantly decreased during the course of pregnancy: 1st screening, 3.48 ± 0.30 μg/mL; 2nd, 2.88 ± 0.27; 3rd, 2.86 ± 0.25; P = 0.0001 (Figure 1).

Bottom Line: FFA/albumin, MCP-1, and HMW adiponectin concentrations were significantly higher in PIH patients than in women with normal pregnancies.The 3 measurements of FFA/albumin showed a significant increase through the course of uncomplicated pregnancies.In contrast, MCP-1 and HMW adiponectin were significantly decreased during the course of pregnancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics & Gynecology, Nara Medical University, Kashihara City, Nara, Japan. naruse@naramed-u.ac.jp

ABSTRACT
Increased insulin resistance and inflammatory action are observed in pregnancy-induced hypertension (PIH), but similar insulin resistance is observed also in successful pregnancy. To estimate insulin resistance and inflammatory activity in normal pregnancy and PIH, serum concentrations of free fatty acids (FFA; corrected with albumin to estimate unbound FFA), monocyte chemoattractant protein (MCP)-1, and high-molecular weight (HMW) adiponectin were measured in severe PIH patients with a BMI less than 25 kg/m(2) and were measured 3 times during the course of pregnancy in women with normal pregnancies. FFA/albumin, MCP-1, and HMW adiponectin concentrations were significantly higher in PIH patients than in women with normal pregnancies. The 3 measurements of FFA/albumin showed a significant increase through the course of uncomplicated pregnancies. In contrast, MCP-1 and HMW adiponectin were significantly decreased during the course of pregnancy. These results suggest that the reduced MCP-1 concentration in normal pregnancy may be a pathway to inhibit the induction of pathological features from physiological insulin resistance and homeostatic inflammation.

Show MeSH
Related in: MedlinePlus