PED/PEA-15 controls fibroblast motility and wound closure by ERK1/2-dependent mechanisms.
Bottom Line: Cultured skin fibroblasts isolated from TgPED mice showed a significant reduction in the ability to recolonize wounded area during scratch assay, compared to control fibroblasts.These changes were accompanied by reduced spreading and decreased formation of stress fibers and focal adhesion plaques.At the molecular level, TgPED fibroblasts displayed decreased RhoA activation and increased abundance of phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2).
Affiliation: Department of Cellular and Molecular Biology and Pathology, Federico II University of Naples, Naples, Italy.Show MeSH
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Mentions: Fibroblast motility is dependent on cell ability to adhere to substrates and to transfer into the cytoplasm the signal that induces the cytoskeleton reorganization (Ridley et al., 2003; Arnaout et al., 2007). TgPED fibroblasts showed no significant difference in adhesion compared to the controls (data not shown). Moreover, after 3 h plating on fibronectin, about 60% of Wt cells exhibited a spread cytoplasm. By contrast, the number of spread cells was about 30% for TgPED (Fig. 3A). To analyze actin cytoskeleton and organization of focal adhesion plaques, Wt and TgPED fibroblasts were stained with rhodamin-conjugated phalloidin or with specific anti-paxillin antibodies, respectively. Clearly, the results showed that at least 70% of TgPED fibroblasts displayed a marked decrease of stress fibers and focal adhesion plaques compared to controls (Fig. 3B).
Affiliation: Department of Cellular and Molecular Biology and Pathology, Federico II University of Naples, Naples, Italy.