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Low densities of serotonin and peptide YY cells in the colon of patients with irritable bowel syndrome.

El-Salhy M, Gundersen D, Ostgaard H, Lomholt-Beck B, Hatlebakk JG, Hausken T - Dig. Dis. Sci. (2011)

Bottom Line: Serotonin and PYY cell densities were reduced in the colon of IBS patients.PP, entroglucagon, and somatostatin-immunoreactive cells were too few to enable reliable quantification.PYY stimulates absorption of water and electrolytes, and inhibits prostaglandin (PG) E2, and vasoactive intestinal peptide, which stimulates intestinal fluid secretion and is a major regulator of the "ileal brake".

View Article: PubMed Central - PubMed

Affiliation: Section for Gastroenterology, Department of Medicine, Stord Helse-Fonna Hospital, Box 4000, 54 09 Stord, Norway. magdy.el-salhy@helse-fonna.no

ABSTRACT

Background: The gut hormones are important in regulating gastrointestinal motility. Disturbances in gastrointestinal motility have been reported in patients with irritable bowel syndrome (IBS). Reduced endocrine cell density, as revealed by chromogranin A, has been reported in the colon of IBS patients.

Aims: To investigate a possible abnormality in the colonic endocrine cells of IBS patients.

Methods: A total of 41 patients with IBS according to Rome Criteria III and 20 controls were included in the study. Biopsies from the right and left colon were obtained from both patients and controls during colonoscopy. The biopsies were immunostained for serotonin, peptide YY (PYY), pancreatic polypeptide (PP), entroglucagon, and somatostatin cells. Cell densities were quantified by computerized image analysis.

Results: Serotonin and PYY cell densities were reduced in the colon of IBS patients. PP, entroglucagon, and somatostatin-immunoreactive cells were too few to enable reliable quantification.

Conclusion: The cause of these observations could be primary genetic defect(s), secondary to altered serotonin and/or PYY signaling systems and/or subclinical inflammation. Serotonin activates the submucosal sensory branch of the enteric nervous system and controls gastrointestinal motility and chloride secretion via interneurons and motor neurons. PYY stimulates absorption of water and electrolytes, and inhibits prostaglandin (PG) E2, and vasoactive intestinal peptide, which stimulates intestinal fluid secretion and is a major regulator of the "ileal brake". Although the cause and effect relationship of these findings is difficult to elucidate, the abnormalities reported here might contribute to the symptoms associated with IBS.

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Related in: MedlinePlus

PYY cell density in the colon in IBS patients and as a whole and in the two subtypes of IBS (a), in the right colon (b), and in the left colon (c). Statistical significance expressed as in Fig. 3
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Fig4: PYY cell density in the colon in IBS patients and as a whole and in the two subtypes of IBS (a), in the right colon (b), and in the left colon (c). Statistical significance expressed as in Fig. 3

Mentions: PYY cell density was lower than controls in the colon as a whole and in the right and left colon. PYY cell density was reduced also in both sub-types of IBS (Figs. 2, 4).Fig. 4


Low densities of serotonin and peptide YY cells in the colon of patients with irritable bowel syndrome.

El-Salhy M, Gundersen D, Ostgaard H, Lomholt-Beck B, Hatlebakk JG, Hausken T - Dig. Dis. Sci. (2011)

PYY cell density in the colon in IBS patients and as a whole and in the two subtypes of IBS (a), in the right colon (b), and in the left colon (c). Statistical significance expressed as in Fig. 3
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3306780&req=5

Fig4: PYY cell density in the colon in IBS patients and as a whole and in the two subtypes of IBS (a), in the right colon (b), and in the left colon (c). Statistical significance expressed as in Fig. 3
Mentions: PYY cell density was lower than controls in the colon as a whole and in the right and left colon. PYY cell density was reduced also in both sub-types of IBS (Figs. 2, 4).Fig. 4

Bottom Line: Serotonin and PYY cell densities were reduced in the colon of IBS patients.PP, entroglucagon, and somatostatin-immunoreactive cells were too few to enable reliable quantification.PYY stimulates absorption of water and electrolytes, and inhibits prostaglandin (PG) E2, and vasoactive intestinal peptide, which stimulates intestinal fluid secretion and is a major regulator of the "ileal brake".

View Article: PubMed Central - PubMed

Affiliation: Section for Gastroenterology, Department of Medicine, Stord Helse-Fonna Hospital, Box 4000, 54 09 Stord, Norway. magdy.el-salhy@helse-fonna.no

ABSTRACT

Background: The gut hormones are important in regulating gastrointestinal motility. Disturbances in gastrointestinal motility have been reported in patients with irritable bowel syndrome (IBS). Reduced endocrine cell density, as revealed by chromogranin A, has been reported in the colon of IBS patients.

Aims: To investigate a possible abnormality in the colonic endocrine cells of IBS patients.

Methods: A total of 41 patients with IBS according to Rome Criteria III and 20 controls were included in the study. Biopsies from the right and left colon were obtained from both patients and controls during colonoscopy. The biopsies were immunostained for serotonin, peptide YY (PYY), pancreatic polypeptide (PP), entroglucagon, and somatostatin cells. Cell densities were quantified by computerized image analysis.

Results: Serotonin and PYY cell densities were reduced in the colon of IBS patients. PP, entroglucagon, and somatostatin-immunoreactive cells were too few to enable reliable quantification.

Conclusion: The cause of these observations could be primary genetic defect(s), secondary to altered serotonin and/or PYY signaling systems and/or subclinical inflammation. Serotonin activates the submucosal sensory branch of the enteric nervous system and controls gastrointestinal motility and chloride secretion via interneurons and motor neurons. PYY stimulates absorption of water and electrolytes, and inhibits prostaglandin (PG) E2, and vasoactive intestinal peptide, which stimulates intestinal fluid secretion and is a major regulator of the "ileal brake". Although the cause and effect relationship of these findings is difficult to elucidate, the abnormalities reported here might contribute to the symptoms associated with IBS.

Show MeSH
Related in: MedlinePlus