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Decreased GABA receptor in the cerebral cortex of epileptic rats: effect of Bacopa monnieri and Bacoside-A.

Mathew J, Balakrishnan S, Antony S, Abraham PM, Paulose CS - J. Biomed. Sci. (2012)

Bottom Line: Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control.GABAAά5 subunit and Cyclic AMP responsible element binding protein were up regulated.Confocal imaging study confirmed the decreased GABA receptors in epileptic rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin-682 022, Kerala, India.

ABSTRACT

Background: Gamma amino butyric acid (GABA), the principal inhibitory neurotransmitter in the cerebral cortex, maintains the inhibitory tones that counter balances neuronal excitation. When this balance is perturbed, seizures may ensue.

Methods: In the present study, alterations of the general GABA, GABAA and GABAB receptors in the cerebral cortex of the epileptic rat and the therapeutic application of Bacopa monnieri were investigated.

Results: Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control. Real Time PCR amplification of GABA receptor subunits such as GABAAά1, GABAAγ, GABAAδ, GABAB and GAD where down regulated (P < 0.001) in epileptic rats. GABAAά5 subunit and Cyclic AMP responsible element binding protein were up regulated. Confocal imaging study confirmed the decreased GABA receptors in epileptic rats. Epileptic rats have deficit in radial arm and Y maze performance.

Conclusions: Bacopa monnieri and Bacoside-A treatment reverses epilepsy associated changes to near control suggesting that decreased GABA receptors in the cerebral cortex have an important role in epileptic occurrence; Bacopa monnieri and Bacoside-A have therapeutic application in epilepsy management.

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Representative graph showing radial arm maze performance of control and experimental rats epileptic rats required more daily trials to achieve three, four, and five consecutive criterion performances. Criterion performance was defined as consumption of the bait in the four baited arms of the radial maze during no more than five entries. There was no significant difference in the average number of trials required by kindled and control rats to achieve the initial criterion performance. C- Control, E- Epileptic, E+B- Epileptic + Bacopa monnieri, E+D- Epileptic + Bacoside-A, E+C- Epileptic + carbamazepine treated rats.
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Figure 8: Representative graph showing radial arm maze performance of control and experimental rats epileptic rats required more daily trials to achieve three, four, and five consecutive criterion performances. Criterion performance was defined as consumption of the bait in the four baited arms of the radial maze during no more than five entries. There was no significant difference in the average number of trials required by kindled and control rats to achieve the initial criterion performance. C- Control, E- Epileptic, E+B- Epileptic + Bacopa monnieri, E+D- Epileptic + Bacoside-A, E+C- Epileptic + carbamazepine treated rats.

Mentions: There was significant increase (p < 0.001) in the number of trials required to achieve five consecutive criterion performances in the epileptic rats compared to control. Treatment using Bacopa monnieri and Bacoside-A reversed this change to near control (Figure 8).


Decreased GABA receptor in the cerebral cortex of epileptic rats: effect of Bacopa monnieri and Bacoside-A.

Mathew J, Balakrishnan S, Antony S, Abraham PM, Paulose CS - J. Biomed. Sci. (2012)

Representative graph showing radial arm maze performance of control and experimental rats epileptic rats required more daily trials to achieve three, four, and five consecutive criterion performances. Criterion performance was defined as consumption of the bait in the four baited arms of the radial maze during no more than five entries. There was no significant difference in the average number of trials required by kindled and control rats to achieve the initial criterion performance. C- Control, E- Epileptic, E+B- Epileptic + Bacopa monnieri, E+D- Epileptic + Bacoside-A, E+C- Epileptic + carbamazepine treated rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3306740&req=5

Figure 8: Representative graph showing radial arm maze performance of control and experimental rats epileptic rats required more daily trials to achieve three, four, and five consecutive criterion performances. Criterion performance was defined as consumption of the bait in the four baited arms of the radial maze during no more than five entries. There was no significant difference in the average number of trials required by kindled and control rats to achieve the initial criterion performance. C- Control, E- Epileptic, E+B- Epileptic + Bacopa monnieri, E+D- Epileptic + Bacoside-A, E+C- Epileptic + carbamazepine treated rats.
Mentions: There was significant increase (p < 0.001) in the number of trials required to achieve five consecutive criterion performances in the epileptic rats compared to control. Treatment using Bacopa monnieri and Bacoside-A reversed this change to near control (Figure 8).

Bottom Line: Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control.GABAAά5 subunit and Cyclic AMP responsible element binding protein were up regulated.Confocal imaging study confirmed the decreased GABA receptors in epileptic rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin-682 022, Kerala, India.

ABSTRACT

Background: Gamma amino butyric acid (GABA), the principal inhibitory neurotransmitter in the cerebral cortex, maintains the inhibitory tones that counter balances neuronal excitation. When this balance is perturbed, seizures may ensue.

Methods: In the present study, alterations of the general GABA, GABAA and GABAB receptors in the cerebral cortex of the epileptic rat and the therapeutic application of Bacopa monnieri were investigated.

Results: Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control. Real Time PCR amplification of GABA receptor subunits such as GABAAά1, GABAAγ, GABAAδ, GABAB and GAD where down regulated (P < 0.001) in epileptic rats. GABAAά5 subunit and Cyclic AMP responsible element binding protein were up regulated. Confocal imaging study confirmed the decreased GABA receptors in epileptic rats. Epileptic rats have deficit in radial arm and Y maze performance.

Conclusions: Bacopa monnieri and Bacoside-A treatment reverses epilepsy associated changes to near control suggesting that decreased GABA receptors in the cerebral cortex have an important role in epileptic occurrence; Bacopa monnieri and Bacoside-A have therapeutic application in epilepsy management.

Show MeSH
Related in: MedlinePlus