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Decreased GABA receptor in the cerebral cortex of epileptic rats: effect of Bacopa monnieri and Bacoside-A.

Mathew J, Balakrishnan S, Antony S, Abraham PM, Paulose CS - J. Biomed. Sci. (2012)

Bottom Line: Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control.GABAAά5 subunit and Cyclic AMP responsible element binding protein were up regulated.Confocal imaging study confirmed the decreased GABA receptors in epileptic rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin-682 022, Kerala, India.

ABSTRACT

Background: Gamma amino butyric acid (GABA), the principal inhibitory neurotransmitter in the cerebral cortex, maintains the inhibitory tones that counter balances neuronal excitation. When this balance is perturbed, seizures may ensue.

Methods: In the present study, alterations of the general GABA, GABAA and GABAB receptors in the cerebral cortex of the epileptic rat and the therapeutic application of Bacopa monnieri were investigated.

Results: Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control. Real Time PCR amplification of GABA receptor subunits such as GABAAά1, GABAAγ, GABAAδ, GABAB and GAD where down regulated (P < 0.001) in epileptic rats. GABAAά5 subunit and Cyclic AMP responsible element binding protein were up regulated. Confocal imaging study confirmed the decreased GABA receptors in epileptic rats. Epileptic rats have deficit in radial arm and Y maze performance.

Conclusions: Bacopa monnieri and Bacoside-A treatment reverses epilepsy associated changes to near control suggesting that decreased GABA receptors in the cerebral cortex have an important role in epileptic occurrence; Bacopa monnieri and Bacoside-A have therapeutic application in epilepsy management.

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Representative graph showing Real-Time amplification of GABA Aγ5 receptor subunit mRNA from the Control and experimental rats. The ΔΔCT method of relative quantification was used to determine the fold change in expression. Values are mean ± S.D of 4-6 separate experiments. C- Control, E- Epileptic, E+B- Epileptic + Bacopa monnieri, E+D- Epileptic + Bacoside-A, E+C- Epileptic + carbamazepine treated rats. ***p < 0.001 when compared to control, @@@p < 0.001 when compared to epileptic group.
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Figure 3: Representative graph showing Real-Time amplification of GABA Aγ5 receptor subunit mRNA from the Control and experimental rats. The ΔΔCT method of relative quantification was used to determine the fold change in expression. Values are mean ± S.D of 4-6 separate experiments. C- Control, E- Epileptic, E+B- Epileptic + Bacopa monnieri, E+D- Epileptic + Bacoside-A, E+C- Epileptic + carbamazepine treated rats. ***p < 0.001 when compared to control, @@@p < 0.001 when compared to epileptic group.

Mentions: Gene expression of GABAAά1, GABAAγ and GABAAδ, where showed significant down regulation (p < 0.001) in the cerebral cortex of the epileptic rats compared to the control. Gene expression of GABAAά5 receptor subunit showed significant up regulation compared to the control. Treatment using Bacopa monnieri, Bacocide-A and Carbamazepine reversed the changes to near control (Figures 1, 2, 3 and 4).


Decreased GABA receptor in the cerebral cortex of epileptic rats: effect of Bacopa monnieri and Bacoside-A.

Mathew J, Balakrishnan S, Antony S, Abraham PM, Paulose CS - J. Biomed. Sci. (2012)

Representative graph showing Real-Time amplification of GABA Aγ5 receptor subunit mRNA from the Control and experimental rats. The ΔΔCT method of relative quantification was used to determine the fold change in expression. Values are mean ± S.D of 4-6 separate experiments. C- Control, E- Epileptic, E+B- Epileptic + Bacopa monnieri, E+D- Epileptic + Bacoside-A, E+C- Epileptic + carbamazepine treated rats. ***p < 0.001 when compared to control, @@@p < 0.001 when compared to epileptic group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3306740&req=5

Figure 3: Representative graph showing Real-Time amplification of GABA Aγ5 receptor subunit mRNA from the Control and experimental rats. The ΔΔCT method of relative quantification was used to determine the fold change in expression. Values are mean ± S.D of 4-6 separate experiments. C- Control, E- Epileptic, E+B- Epileptic + Bacopa monnieri, E+D- Epileptic + Bacoside-A, E+C- Epileptic + carbamazepine treated rats. ***p < 0.001 when compared to control, @@@p < 0.001 when compared to epileptic group.
Mentions: Gene expression of GABAAά1, GABAAγ and GABAAδ, where showed significant down regulation (p < 0.001) in the cerebral cortex of the epileptic rats compared to the control. Gene expression of GABAAά5 receptor subunit showed significant up regulation compared to the control. Treatment using Bacopa monnieri, Bacocide-A and Carbamazepine reversed the changes to near control (Figures 1, 2, 3 and 4).

Bottom Line: Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control.GABAAά5 subunit and Cyclic AMP responsible element binding protein were up regulated.Confocal imaging study confirmed the decreased GABA receptors in epileptic rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin-682 022, Kerala, India.

ABSTRACT

Background: Gamma amino butyric acid (GABA), the principal inhibitory neurotransmitter in the cerebral cortex, maintains the inhibitory tones that counter balances neuronal excitation. When this balance is perturbed, seizures may ensue.

Methods: In the present study, alterations of the general GABA, GABAA and GABAB receptors in the cerebral cortex of the epileptic rat and the therapeutic application of Bacopa monnieri were investigated.

Results: Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control. Real Time PCR amplification of GABA receptor subunits such as GABAAά1, GABAAγ, GABAAδ, GABAB and GAD where down regulated (P < 0.001) in epileptic rats. GABAAά5 subunit and Cyclic AMP responsible element binding protein were up regulated. Confocal imaging study confirmed the decreased GABA receptors in epileptic rats. Epileptic rats have deficit in radial arm and Y maze performance.

Conclusions: Bacopa monnieri and Bacoside-A treatment reverses epilepsy associated changes to near control suggesting that decreased GABA receptors in the cerebral cortex have an important role in epileptic occurrence; Bacopa monnieri and Bacoside-A have therapeutic application in epilepsy management.

Show MeSH
Related in: MedlinePlus