Limits...
Decreased GABA receptor in the cerebral cortex of epileptic rats: effect of Bacopa monnieri and Bacoside-A.

Mathew J, Balakrishnan S, Antony S, Abraham PM, Paulose CS - J. Biomed. Sci. (2012)

Bottom Line: Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control.GABAAά5 subunit and Cyclic AMP responsible element binding protein were up regulated.Confocal imaging study confirmed the decreased GABA receptors in epileptic rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin-682 022, Kerala, India.

ABSTRACT

Background: Gamma amino butyric acid (GABA), the principal inhibitory neurotransmitter in the cerebral cortex, maintains the inhibitory tones that counter balances neuronal excitation. When this balance is perturbed, seizures may ensue.

Methods: In the present study, alterations of the general GABA, GABAA and GABAB receptors in the cerebral cortex of the epileptic rat and the therapeutic application of Bacopa monnieri were investigated.

Results: Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control. Real Time PCR amplification of GABA receptor subunits such as GABAAά1, GABAAγ, GABAAδ, GABAB and GAD where down regulated (P < 0.001) in epileptic rats. GABAAά5 subunit and Cyclic AMP responsible element binding protein were up regulated. Confocal imaging study confirmed the decreased GABA receptors in epileptic rats. Epileptic rats have deficit in radial arm and Y maze performance.

Conclusions: Bacopa monnieri and Bacoside-A treatment reverses epilepsy associated changes to near control suggesting that decreased GABA receptors in the cerebral cortex have an important role in epileptic occurrence; Bacopa monnieri and Bacoside-A have therapeutic application in epilepsy management.

Show MeSH

Related in: MedlinePlus

GABAAα1 receptor subunit antibody staining in the cerebral cortex of Control. and experimental rats. C- Control, E- Epileptic, E+B- Epileptic + Bacopa monnieri, E+D- Epileptic + Bacoside-A, E+C- Epileptic + carbamazepine treated rats. Pixel intensity of experimental rats were C-103465 ± 3043, E- 76456 ± 1593**, E+B-95835@@ ± 2935, E+D-90564 ± 2553@@, E+C-87593 ± 1093@@. () Indicating the GABAAα1 receptor subunit. **p < 0.01 when compared to control, @@p < 0.01 when compared to epileptic group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3306740&req=5

Figure 10: GABAAα1 receptor subunit antibody staining in the cerebral cortex of Control. and experimental rats. C- Control, E- Epileptic, E+B- Epileptic + Bacopa monnieri, E+D- Epileptic + Bacoside-A, E+C- Epileptic + carbamazepine treated rats. Pixel intensity of experimental rats were C-103465 ± 3043, E- 76456 ± 1593**, E+B-95835@@ ± 2935, E+D-90564 ± 2553@@, E+C-87593 ± 1093@@. () Indicating the GABAAα1 receptor subunit. **p < 0.01 when compared to control, @@p < 0.01 when compared to epileptic group.

Mentions: GABAAα1 receptor subunit antibody staining in the hippocampus showed a significant decrease (p < 0.01) in the GABAAα1 receptor subunit in epileptic rat compared to control. Bacopa monnieri, Bacoside-A and Carbamazepine treated epileptic rats showed a significant reversal (p < 0.05) of the decrease in GABAAα1 receptor subunit staining in the hippocampus compared to epileptic rats (Figure 10).


Decreased GABA receptor in the cerebral cortex of epileptic rats: effect of Bacopa monnieri and Bacoside-A.

Mathew J, Balakrishnan S, Antony S, Abraham PM, Paulose CS - J. Biomed. Sci. (2012)

GABAAα1 receptor subunit antibody staining in the cerebral cortex of Control. and experimental rats. C- Control, E- Epileptic, E+B- Epileptic + Bacopa monnieri, E+D- Epileptic + Bacoside-A, E+C- Epileptic + carbamazepine treated rats. Pixel intensity of experimental rats were C-103465 ± 3043, E- 76456 ± 1593**, E+B-95835@@ ± 2935, E+D-90564 ± 2553@@, E+C-87593 ± 1093@@. () Indicating the GABAAα1 receptor subunit. **p < 0.01 when compared to control, @@p < 0.01 when compared to epileptic group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3306740&req=5

Figure 10: GABAAα1 receptor subunit antibody staining in the cerebral cortex of Control. and experimental rats. C- Control, E- Epileptic, E+B- Epileptic + Bacopa monnieri, E+D- Epileptic + Bacoside-A, E+C- Epileptic + carbamazepine treated rats. Pixel intensity of experimental rats were C-103465 ± 3043, E- 76456 ± 1593**, E+B-95835@@ ± 2935, E+D-90564 ± 2553@@, E+C-87593 ± 1093@@. () Indicating the GABAAα1 receptor subunit. **p < 0.01 when compared to control, @@p < 0.01 when compared to epileptic group.
Mentions: GABAAα1 receptor subunit antibody staining in the hippocampus showed a significant decrease (p < 0.01) in the GABAAα1 receptor subunit in epileptic rat compared to control. Bacopa monnieri, Bacoside-A and Carbamazepine treated epileptic rats showed a significant reversal (p < 0.05) of the decrease in GABAAα1 receptor subunit staining in the hippocampus compared to epileptic rats (Figure 10).

Bottom Line: Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control.GABAAά5 subunit and Cyclic AMP responsible element binding protein were up regulated.Confocal imaging study confirmed the decreased GABA receptors in epileptic rats.

View Article: PubMed Central - HTML - PubMed

Affiliation: Molecular Neurobiology and Cell Biology Unit, Centre for Neuroscience, Department of Biotechnology, Cochin University of Science and Technology, Cochin-682 022, Kerala, India.

ABSTRACT

Background: Gamma amino butyric acid (GABA), the principal inhibitory neurotransmitter in the cerebral cortex, maintains the inhibitory tones that counter balances neuronal excitation. When this balance is perturbed, seizures may ensue.

Methods: In the present study, alterations of the general GABA, GABAA and GABAB receptors in the cerebral cortex of the epileptic rat and the therapeutic application of Bacopa monnieri were investigated.

Results: Scatchard analysis of [3H]GABA, [3H]bicuculline and [3H]baclofen in the cerebral cortex of the epileptic rat showed significant decrease in Bmax (P < 0.001) compared to control. Real Time PCR amplification of GABA receptor subunits such as GABAAά1, GABAAγ, GABAAδ, GABAB and GAD where down regulated (P < 0.001) in epileptic rats. GABAAά5 subunit and Cyclic AMP responsible element binding protein were up regulated. Confocal imaging study confirmed the decreased GABA receptors in epileptic rats. Epileptic rats have deficit in radial arm and Y maze performance.

Conclusions: Bacopa monnieri and Bacoside-A treatment reverses epilepsy associated changes to near control suggesting that decreased GABA receptors in the cerebral cortex have an important role in epileptic occurrence; Bacopa monnieri and Bacoside-A have therapeutic application in epilepsy management.

Show MeSH
Related in: MedlinePlus