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The Wnt secretion protein Evi/Gpr177 promotes glioma tumourigenesis.

Augustin I, Goidts V, Bongers A, Kerr G, Vollert G, Radlwimmer B, Hartmann C, Herold-Mende C, Reifenberger G, von Deimling A, Boutros M - EMBO Mol Med (2011)

Bottom Line: Malignant astrocytomas are highly aggressive brain tumours with poor prognosis.Evi/Wls is a core Wnt signalling component and a specific regulator of pan-Wnt protein secretion, affecting both canonical and non-canonical signalling.We further show that Evi/Wls overexpression is sufficient to promote downstream Wnt signalling.

View Article: PubMed Central - PubMed

Affiliation: German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics and Heidelberg University, Faculty of Medicine Mannheim, Department of Cell and Molecular Biology, Heidelberg, Germany. i.augustin@dkfz.de

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Wnt secretion is important for tumour cell migration and tumour formation in xenograft modelsEvi shRNA U87MG cells showed less transwell migration compared to control. Similar effect was achieved by siRNA transfection.Migration experiments were done as short-term assays to exclude anti-proliferative effects. Values represent mean ± SD from three-independent experiments (*p < 0.05; **p < 0.01).Reduced in vivo growth of glioma cells transfected with shRNA targeting Evi. The appearance of U87MG glioma xenografts formed by Evi shRNA or control transfected cells was reduced in the Evi downregulated glioma cells.
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fig05: Wnt secretion is important for tumour cell migration and tumour formation in xenograft modelsEvi shRNA U87MG cells showed less transwell migration compared to control. Similar effect was achieved by siRNA transfection.Migration experiments were done as short-term assays to exclude anti-proliferative effects. Values represent mean ± SD from three-independent experiments (*p < 0.05; **p < 0.01).Reduced in vivo growth of glioma cells transfected with shRNA targeting Evi. The appearance of U87MG glioma xenografts formed by Evi shRNA or control transfected cells was reduced in the Evi downregulated glioma cells.

Mentions: Glioblastoma are characterized by pronounced invasion of tumour cells into the surrounding healthy tissue (Tysnes & Mahesparan, 2001). To examine the consequences of Evi depletion in glioma cells on cell migration, we performed transwell migration experiments. As shown in Fig 5A and B, siRNA-based silencing of Evi expression caused a 32% inhibition of migration of glioma cells; a more robust lentiviral shRNA-induced downregulation of Evi led to an even stronger 63% decrease in migratory behaviour.


The Wnt secretion protein Evi/Gpr177 promotes glioma tumourigenesis.

Augustin I, Goidts V, Bongers A, Kerr G, Vollert G, Radlwimmer B, Hartmann C, Herold-Mende C, Reifenberger G, von Deimling A, Boutros M - EMBO Mol Med (2011)

Wnt secretion is important for tumour cell migration and tumour formation in xenograft modelsEvi shRNA U87MG cells showed less transwell migration compared to control. Similar effect was achieved by siRNA transfection.Migration experiments were done as short-term assays to exclude anti-proliferative effects. Values represent mean ± SD from three-independent experiments (*p < 0.05; **p < 0.01).Reduced in vivo growth of glioma cells transfected with shRNA targeting Evi. The appearance of U87MG glioma xenografts formed by Evi shRNA or control transfected cells was reduced in the Evi downregulated glioma cells.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3306557&req=5

fig05: Wnt secretion is important for tumour cell migration and tumour formation in xenograft modelsEvi shRNA U87MG cells showed less transwell migration compared to control. Similar effect was achieved by siRNA transfection.Migration experiments were done as short-term assays to exclude anti-proliferative effects. Values represent mean ± SD from three-independent experiments (*p < 0.05; **p < 0.01).Reduced in vivo growth of glioma cells transfected with shRNA targeting Evi. The appearance of U87MG glioma xenografts formed by Evi shRNA or control transfected cells was reduced in the Evi downregulated glioma cells.
Mentions: Glioblastoma are characterized by pronounced invasion of tumour cells into the surrounding healthy tissue (Tysnes & Mahesparan, 2001). To examine the consequences of Evi depletion in glioma cells on cell migration, we performed transwell migration experiments. As shown in Fig 5A and B, siRNA-based silencing of Evi expression caused a 32% inhibition of migration of glioma cells; a more robust lentiviral shRNA-induced downregulation of Evi led to an even stronger 63% decrease in migratory behaviour.

Bottom Line: Malignant astrocytomas are highly aggressive brain tumours with poor prognosis.Evi/Wls is a core Wnt signalling component and a specific regulator of pan-Wnt protein secretion, affecting both canonical and non-canonical signalling.We further show that Evi/Wls overexpression is sufficient to promote downstream Wnt signalling.

View Article: PubMed Central - PubMed

Affiliation: German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics and Heidelberg University, Faculty of Medicine Mannheim, Department of Cell and Molecular Biology, Heidelberg, Germany. i.augustin@dkfz.de

Show MeSH
Related in: MedlinePlus