The Wnt secretion protein Evi/Gpr177 promotes glioma tumourigenesis.
Bottom Line: Malignant astrocytomas are highly aggressive brain tumours with poor prognosis.Evi/Wls is a core Wnt signalling component and a specific regulator of pan-Wnt protein secretion, affecting both canonical and non-canonical signalling.We further show that Evi/Wls overexpression is sufficient to promote downstream Wnt signalling.
Affiliation: German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics and Heidelberg University, Faculty of Medicine Mannheim, Department of Cell and Molecular Biology, Heidelberg, Germany. firstname.lastname@example.orgShow MeSH
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Mentions: Light microscopic analysis of SLGC spheres transduced with Evi shRNA versus control shRNA revealed strong morphological differences: Evi silenced spheres were significantly smaller and lost their packed condensed morphology compared to control spheres (Fig 4A and Fig S4 of Supporting information). Moreover, the amount of viable cells was significantly reduced in Evi depleted spheroid cultures (Fig 4B). Further analysis of apoptotic cells after Evi shRNA transduction revealed an increase in apoptosis as demonstrated by a significant rise in the sub-G1 fraction with no significant changes in cell cycle distribution (Fig 4C and D). These experiments demonstrate that Evi is required for cell survival in primary patient-derived glioblastoma cells.
Affiliation: German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics and Heidelberg University, Faculty of Medicine Mannheim, Department of Cell and Molecular Biology, Heidelberg, Germany. email@example.com