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Effects of blood products on inflammatory response in endothelial cells in vitro.

Urner M, Herrmann IK, Buddeberg F, Schuppli C, Roth Z'graggen B, Hasler M, Schanz U, Mehr M, Spahn DR, Beck Schimmer B - PLoS ONE (2012)

Bottom Line: Platelet concentrates, and to a lesser extent also FFP, caused the most pronounced response, which was accentuated in already pre-stimulated endothelial cells.Inflammation upon blood product exposure is strongly accentuated when endothelial cells are pre-injured.High lipid contents in the respective blood products goes along with an accentuated inflammatory reaction from endothelial cells.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anesthesiology, University Hospital Zurich, Zurich, Switzerland.

ABSTRACT

Background: Transfusing blood products may induce inflammatory reactions within the vascular compartment potentially leading to a systemic inflammatory response. Experiments were designed to assess the inflammatory potential of different blood products in an endothelial cell-based in vitro model and to compare baseline levels of potentially activating substances in transfusion products.

Methods: The inflammatory response from pre-activated (endotoxin-stimulated) and non-activated endothelial cells as well as neutrophil endothelial transmigration in response to packed red blood cells (PRBC), platelet concentrates (PC) and fresh frozen plasma (FFP) was determined. Baseline inflammatory mediator and lipid concentrations in blood products were evaluated.

Results: Following incubation with all blood products, an increased inflammatory mediator release from endothelial cells was observed. Platelet concentrates, and to a lesser extent also FFP, caused the most pronounced response, which was accentuated in already pre-stimulated endothelial cells. Inflammatory response of endothelial cells as well as blood product-induced migration of neutrophils through the endothelium was in good agreement with the lipid content of the according blood product.

Conclusion: Within the group of different blood transfusion products both PC and FFP have a high inflammatory potential with regard to activation of endothelial cells. Inflammation upon blood product exposure is strongly accentuated when endothelial cells are pre-injured. High lipid contents in the respective blood products goes along with an accentuated inflammatory reaction from endothelial cells.

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Storage-dependency of lipid concentration in blood products.Storage-dependent concentration of lipids in packed red blood cells (PRBC) (A), and in pooled (B) and apheresis platelet concentrates (C). Concentrations of lipids were determined using the sulfo-vanillin colorimetric method. Results are shown as medians with interquartile ranges.
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pone-0033403-g004: Storage-dependency of lipid concentration in blood products.Storage-dependent concentration of lipids in packed red blood cells (PRBC) (A), and in pooled (B) and apheresis platelet concentrates (C). Concentrations of lipids were determined using the sulfo-vanillin colorimetric method. Results are shown as medians with interquartile ranges.

Mentions: To reveal potential storage-dependent changes in lipid content (breakdown products of membrane lipids), lipid concentrations were assessed in PRBC and PC. In PRBC no significant changes were observed over a storage period of up to 42 days (Spearman's rank correlation coefficient: 0.214; p-value: 0.180; Fig. 4 and Table S6). Regarding pooled and apheresis PC, a storage-age dependent increase in lipid concentrations after 6 days of storage was found (Fig. 4 and Table S6). The storage age had no significant influence on neutrophil transmigration (Table S7).


Effects of blood products on inflammatory response in endothelial cells in vitro.

Urner M, Herrmann IK, Buddeberg F, Schuppli C, Roth Z'graggen B, Hasler M, Schanz U, Mehr M, Spahn DR, Beck Schimmer B - PLoS ONE (2012)

Storage-dependency of lipid concentration in blood products.Storage-dependent concentration of lipids in packed red blood cells (PRBC) (A), and in pooled (B) and apheresis platelet concentrates (C). Concentrations of lipids were determined using the sulfo-vanillin colorimetric method. Results are shown as medians with interquartile ranges.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3306413&req=5

pone-0033403-g004: Storage-dependency of lipid concentration in blood products.Storage-dependent concentration of lipids in packed red blood cells (PRBC) (A), and in pooled (B) and apheresis platelet concentrates (C). Concentrations of lipids were determined using the sulfo-vanillin colorimetric method. Results are shown as medians with interquartile ranges.
Mentions: To reveal potential storage-dependent changes in lipid content (breakdown products of membrane lipids), lipid concentrations were assessed in PRBC and PC. In PRBC no significant changes were observed over a storage period of up to 42 days (Spearman's rank correlation coefficient: 0.214; p-value: 0.180; Fig. 4 and Table S6). Regarding pooled and apheresis PC, a storage-age dependent increase in lipid concentrations after 6 days of storage was found (Fig. 4 and Table S6). The storage age had no significant influence on neutrophil transmigration (Table S7).

Bottom Line: Platelet concentrates, and to a lesser extent also FFP, caused the most pronounced response, which was accentuated in already pre-stimulated endothelial cells.Inflammation upon blood product exposure is strongly accentuated when endothelial cells are pre-injured.High lipid contents in the respective blood products goes along with an accentuated inflammatory reaction from endothelial cells.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anesthesiology, University Hospital Zurich, Zurich, Switzerland.

ABSTRACT

Background: Transfusing blood products may induce inflammatory reactions within the vascular compartment potentially leading to a systemic inflammatory response. Experiments were designed to assess the inflammatory potential of different blood products in an endothelial cell-based in vitro model and to compare baseline levels of potentially activating substances in transfusion products.

Methods: The inflammatory response from pre-activated (endotoxin-stimulated) and non-activated endothelial cells as well as neutrophil endothelial transmigration in response to packed red blood cells (PRBC), platelet concentrates (PC) and fresh frozen plasma (FFP) was determined. Baseline inflammatory mediator and lipid concentrations in blood products were evaluated.

Results: Following incubation with all blood products, an increased inflammatory mediator release from endothelial cells was observed. Platelet concentrates, and to a lesser extent also FFP, caused the most pronounced response, which was accentuated in already pre-stimulated endothelial cells. Inflammatory response of endothelial cells as well as blood product-induced migration of neutrophils through the endothelium was in good agreement with the lipid content of the according blood product.

Conclusion: Within the group of different blood transfusion products both PC and FFP have a high inflammatory potential with regard to activation of endothelial cells. Inflammation upon blood product exposure is strongly accentuated when endothelial cells are pre-injured. High lipid contents in the respective blood products goes along with an accentuated inflammatory reaction from endothelial cells.

Show MeSH
Related in: MedlinePlus