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Seawater acidification and elevated temperature affect gene expression patterns of the pearl oyster Pinctada fucata.

Liu W, Huang X, Lin J, He M - PLoS ONE (2012)

Bottom Line: Oceanic uptake of anthropogenic carbon dioxide results in decrease in seawater pH and increase in temperature.The results showed that under warming and acidic seawater conditions, expression of aspein and calmodulin showed no significant differences among different time point in condition 8.10 T.These results demonstrate that the declined seawater pH and elevated temperature will impact the physiological process, and potentially the adaptability of P. fucata to future warming and acidified ocean.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.

ABSTRACT
Oceanic uptake of anthropogenic carbon dioxide results in decrease in seawater pH and increase in temperature. In this study, we demonstrated the synergistic effects of elevated seawater temperature and declined seawater pH on gene expression patterns of aspein, calmodulin, nacrein, she-7-F10 and hsp70 in the pearl oyster Pinctada fucata. Under 'business-as-usual' scenarios, four treatments were examined: (1) ambient pH (8.10) and ambient temperature (27 °C) (control condition), (2) ambient pH and elevated temperature (+3 °C), (3) declined pH (7.70) and ambient temperature, (4) declined pH and elevated temperature. The results showed that under warming and acidic seawater conditions, expression of aspein and calmodulin showed no significant differences among different time point in condition 8.10 T. But the levels of aspein and calmodulin in conditions 8.10 T+3, 7.70 T and 7.70 T+3, and levels of nacrein, she-7-F10 in all the four treatments changed significantly. Low pH and pH × temperature interaction influenced the expression of aspein and calmodulin significantly after hours 48 and 96. Significant effects of low pH and pH × temperature interaction on the expression of nacrein were observed at hour 96. The expression level of she-7-F10 was affected significantly by pH after hours 48 and 96. The expression of hsp70 was significantly affected by temperature, pH, temperature × pH interaction at hour 6, and by temperature × pH interaction at hour 24. This study suggested that declined pH and pH × temperature interaction induced down regulation of calcification related genes, and the interaction between declined seawater pH and elevated temperature caused up regulation of hsp70 in P. facata. These results demonstrate that the declined seawater pH and elevated temperature will impact the physiological process, and potentially the adaptability of P. fucata to future warming and acidified ocean.

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Real-time PCR analysis of expression of aspein in response to elevated temperature and declined pH.
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pone-0033679-g001: Real-time PCR analysis of expression of aspein in response to elevated temperature and declined pH.

Mentions: The relative expression level of aspein fluctuated gradually during hours 0–96 in condition 8.10 T and showed no significant difference among different time point (p>0.1). In conditions 8.10 T+3, 7.70 T and 7.70 T+3, the levels decreased significantly to minimum values on hour 96 (p<0.1) (Fig. 1, Table 2). The expression of aspein was significantly affected by pH and pH×temperature interaction at hours 48 and 96 (p<0.1) (Table 3). The expression was significantly lower in condition 7.70 T and 7.70 T+3 than in condition 8.10 T and 8.10 T+3 at hours 48 and 96 (Fig. 1).


Seawater acidification and elevated temperature affect gene expression patterns of the pearl oyster Pinctada fucata.

Liu W, Huang X, Lin J, He M - PLoS ONE (2012)

Real-time PCR analysis of expression of aspein in response to elevated temperature and declined pH.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3306283&req=5

pone-0033679-g001: Real-time PCR analysis of expression of aspein in response to elevated temperature and declined pH.
Mentions: The relative expression level of aspein fluctuated gradually during hours 0–96 in condition 8.10 T and showed no significant difference among different time point (p>0.1). In conditions 8.10 T+3, 7.70 T and 7.70 T+3, the levels decreased significantly to minimum values on hour 96 (p<0.1) (Fig. 1, Table 2). The expression of aspein was significantly affected by pH and pH×temperature interaction at hours 48 and 96 (p<0.1) (Table 3). The expression was significantly lower in condition 7.70 T and 7.70 T+3 than in condition 8.10 T and 8.10 T+3 at hours 48 and 96 (Fig. 1).

Bottom Line: Oceanic uptake of anthropogenic carbon dioxide results in decrease in seawater pH and increase in temperature.The results showed that under warming and acidic seawater conditions, expression of aspein and calmodulin showed no significant differences among different time point in condition 8.10 T.These results demonstrate that the declined seawater pH and elevated temperature will impact the physiological process, and potentially the adaptability of P. fucata to future warming and acidified ocean.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.

ABSTRACT
Oceanic uptake of anthropogenic carbon dioxide results in decrease in seawater pH and increase in temperature. In this study, we demonstrated the synergistic effects of elevated seawater temperature and declined seawater pH on gene expression patterns of aspein, calmodulin, nacrein, she-7-F10 and hsp70 in the pearl oyster Pinctada fucata. Under 'business-as-usual' scenarios, four treatments were examined: (1) ambient pH (8.10) and ambient temperature (27 °C) (control condition), (2) ambient pH and elevated temperature (+3 °C), (3) declined pH (7.70) and ambient temperature, (4) declined pH and elevated temperature. The results showed that under warming and acidic seawater conditions, expression of aspein and calmodulin showed no significant differences among different time point in condition 8.10 T. But the levels of aspein and calmodulin in conditions 8.10 T+3, 7.70 T and 7.70 T+3, and levels of nacrein, she-7-F10 in all the four treatments changed significantly. Low pH and pH × temperature interaction influenced the expression of aspein and calmodulin significantly after hours 48 and 96. Significant effects of low pH and pH × temperature interaction on the expression of nacrein were observed at hour 96. The expression level of she-7-F10 was affected significantly by pH after hours 48 and 96. The expression of hsp70 was significantly affected by temperature, pH, temperature × pH interaction at hour 6, and by temperature × pH interaction at hour 24. This study suggested that declined pH and pH × temperature interaction induced down regulation of calcification related genes, and the interaction between declined seawater pH and elevated temperature caused up regulation of hsp70 in P. facata. These results demonstrate that the declined seawater pH and elevated temperature will impact the physiological process, and potentially the adaptability of P. fucata to future warming and acidified ocean.

Show MeSH
Related in: MedlinePlus