Limits...
Both FA- and mPEG-conjugated chitosan nanoparticles for targeted cellular uptake and enhanced tumor tissue distribution.

Hou Z, Zhan C, Jiang Q, Hu Q, Li L, Chang D, Yang X, Wang Y, Li Y, Ye S, Xie L, Yi Y, Zhang Q - Nanoscale Res Lett (2011)

Bottom Line: Results show that the chitosan NPs presented a narrow-size distribution with an average diameter about 200 nm regardless of the type of functional group.In addition, MMC was easily loaded to the mPEG-FA-NPs with drug-loading content of 9.1%, and the drug releases were biphasic with an initial burst release, followed by a subsequent slower release.Laser confocal scanning imaging proved that both mPEG-FA-NPs and FA-NPs could greatly enhance uptake by HeLa cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: First Hospital, Xiamen University, Xiamen, 361003, China. Xly885@163.com.

ABSTRACT
Both folic acid (FA)- and methoxypoly(ethylene glycol) (mPEG)-conjugated chitosan nanoparticles (NPs) had been designed for targeted and prolong anticancer drug delivery system. The chitosan NPs were prepared with combination of ionic gelation and chemical cross-linking method, followed by conjugation with both FA and mPEG, respectively. FA-mPEG-NPs were compared with either NPs or mPEG-/FA-NPs in terms of their size, targeting cellular efficiency and tumor tissue distribution. The specificity of the mPEG-FA-NPs targeting cancerous cells was demonstrated by comparative intracellular uptake of NPs and mPEG-/FA-NPs by human adenocarcinoma HeLa cells. Mitomycin C (MMC), as a model drug, was loaded to the mPEG-FA-NPs. Results show that the chitosan NPs presented a narrow-size distribution with an average diameter about 200 nm regardless of the type of functional group. In addition, MMC was easily loaded to the mPEG-FA-NPs with drug-loading content of 9.1%, and the drug releases were biphasic with an initial burst release, followed by a subsequent slower release. Laser confocal scanning imaging proved that both mPEG-FA-NPs and FA-NPs could greatly enhance uptake by HeLa cells. In vivo animal experiments, using a nude mice xenograft model, demonstrated that an increased amount of mPEG-FA-NPs or FA-NPs were accumulated in the tumor tissue relative to the mPEG-NPs or NPs alone. These results suggest that both FA- and mPEG-conjugated chitosan NPs are potentially prolonged drug delivery system for tumor cell-selective targeting treatments.

No MeSH data available.


Related in: MedlinePlus

Schematic illustration of the preparation of NPs and the modification of NPs. GA, glutaraldehyde; EDC, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride; STPP, sodium triphosphate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3306018&req=5

Figure 1: Schematic illustration of the preparation of NPs and the modification of NPs. GA, glutaraldehyde; EDC, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride; STPP, sodium triphosphate.

Mentions: In this paper, we aim at conjugating both FA and mPEG to the surface of chitosan NPs in order to reach their target, prolong blood circulation, and reduce phagocytosis. Either FA- or mPEG-modified chitosan NPs (FA-NPs or mPEG-NPs) were also prepared for comparison. We chose mitomycin C (MMC) as model drugs to prepare drug-loaded chitosan NPs (MMC-mPEG-FA-NPs) through a covalent coupling. The preparation of NPs and the modification of NPs are illustrated in Figure 1.


Both FA- and mPEG-conjugated chitosan nanoparticles for targeted cellular uptake and enhanced tumor tissue distribution.

Hou Z, Zhan C, Jiang Q, Hu Q, Li L, Chang D, Yang X, Wang Y, Li Y, Ye S, Xie L, Yi Y, Zhang Q - Nanoscale Res Lett (2011)

Schematic illustration of the preparation of NPs and the modification of NPs. GA, glutaraldehyde; EDC, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride; STPP, sodium triphosphate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3306018&req=5

Figure 1: Schematic illustration of the preparation of NPs and the modification of NPs. GA, glutaraldehyde; EDC, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride; STPP, sodium triphosphate.
Mentions: In this paper, we aim at conjugating both FA and mPEG to the surface of chitosan NPs in order to reach their target, prolong blood circulation, and reduce phagocytosis. Either FA- or mPEG-modified chitosan NPs (FA-NPs or mPEG-NPs) were also prepared for comparison. We chose mitomycin C (MMC) as model drugs to prepare drug-loaded chitosan NPs (MMC-mPEG-FA-NPs) through a covalent coupling. The preparation of NPs and the modification of NPs are illustrated in Figure 1.

Bottom Line: Results show that the chitosan NPs presented a narrow-size distribution with an average diameter about 200 nm regardless of the type of functional group.In addition, MMC was easily loaded to the mPEG-FA-NPs with drug-loading content of 9.1%, and the drug releases were biphasic with an initial burst release, followed by a subsequent slower release.Laser confocal scanning imaging proved that both mPEG-FA-NPs and FA-NPs could greatly enhance uptake by HeLa cells.

View Article: PubMed Central - HTML - PubMed

Affiliation: First Hospital, Xiamen University, Xiamen, 361003, China. Xly885@163.com.

ABSTRACT
Both folic acid (FA)- and methoxypoly(ethylene glycol) (mPEG)-conjugated chitosan nanoparticles (NPs) had been designed for targeted and prolong anticancer drug delivery system. The chitosan NPs were prepared with combination of ionic gelation and chemical cross-linking method, followed by conjugation with both FA and mPEG, respectively. FA-mPEG-NPs were compared with either NPs or mPEG-/FA-NPs in terms of their size, targeting cellular efficiency and tumor tissue distribution. The specificity of the mPEG-FA-NPs targeting cancerous cells was demonstrated by comparative intracellular uptake of NPs and mPEG-/FA-NPs by human adenocarcinoma HeLa cells. Mitomycin C (MMC), as a model drug, was loaded to the mPEG-FA-NPs. Results show that the chitosan NPs presented a narrow-size distribution with an average diameter about 200 nm regardless of the type of functional group. In addition, MMC was easily loaded to the mPEG-FA-NPs with drug-loading content of 9.1%, and the drug releases were biphasic with an initial burst release, followed by a subsequent slower release. Laser confocal scanning imaging proved that both mPEG-FA-NPs and FA-NPs could greatly enhance uptake by HeLa cells. In vivo animal experiments, using a nude mice xenograft model, demonstrated that an increased amount of mPEG-FA-NPs or FA-NPs were accumulated in the tumor tissue relative to the mPEG-NPs or NPs alone. These results suggest that both FA- and mPEG-conjugated chitosan NPs are potentially prolonged drug delivery system for tumor cell-selective targeting treatments.

No MeSH data available.


Related in: MedlinePlus