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Parametric analysis of a novel semi-circular microfluidic CD-ELISA valve.

Lin SI - J Biol Eng (2011)

Bottom Line: Together with supporting experiments, simulation based on two-phase flow theory is used in this study, and the feasibility of this novel valve design is confirmed.From both the experimental results and the simulated results, it is evident that the narrowest channel width and the contact angle are the primary factors influencing valve burst frequency.These can be used as the main controlling factors during the design.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Power Mechanical Engineering, National Formosa University, Taiwan. samlin7@ms41.hinet.net.

ABSTRACT
CD-ELISA uses the microfluidic ranking method and centrifugal force to control the testing solution as it flows into the reaction region. The most challenging part of CD-ELISA is controlling the flow process for different biological testing solutions, i.e. the controlling sequence for the microfluidic channel valves. The microfluidic channel valve is therefore the most important fluid channel structure for CD-ELISA. In this study, we propose a valve design suitable for a wide range rotational speeds which can be applied for mass production (molding). Together with supporting experiments, simulation based on two-phase flow theory is used in this study, and the feasibility of this novel valve design is confirmed. Influencing design factors for the microfluidic channel valves in CD-ELISA are investigated, including various shapes of the arc, distance d, radius r, the location of the center of the circle, and the contact angle. From both the experimental results and the simulated results, it is evident that the narrowest channel width and the contact angle are the primary factors influencing valve burst frequency. These can be used as the main controlling factors during the design.

No MeSH data available.


Corresponding diagram for the Smaller The Better (STB) characteristic.
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Figure 10: Corresponding diagram for the Smaller The Better (STB) characteristic.

Mentions: Based on this selection principle, the values which optimize the design are selected, and the minimum and maximum average S/N ratio for each factor level is searched. Figure 9 shows the corresponding factor diagram for the 'Larger The Better' (LTB) characteristic. For the level of optimal LTB factors, the combination is A3 = 0.12, B2 = 0.08, and C1 = 0.155. Figure 10 shows the corresponding factor diagram for the 'Smaller The Better' (STB) characteristic. For the level of optimal STB factors, the combination is A2 = 1, B1 = 0.06, and C1 = 0.175. The optimal combination can be obtained from the eighth combination in orthogonal arrays L9 (33) in Table 3, which indicates a higher rotational speed.


Parametric analysis of a novel semi-circular microfluidic CD-ELISA valve.

Lin SI - J Biol Eng (2011)

Corresponding diagram for the Smaller The Better (STB) characteristic.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3306004&req=5

Figure 10: Corresponding diagram for the Smaller The Better (STB) characteristic.
Mentions: Based on this selection principle, the values which optimize the design are selected, and the minimum and maximum average S/N ratio for each factor level is searched. Figure 9 shows the corresponding factor diagram for the 'Larger The Better' (LTB) characteristic. For the level of optimal LTB factors, the combination is A3 = 0.12, B2 = 0.08, and C1 = 0.155. Figure 10 shows the corresponding factor diagram for the 'Smaller The Better' (STB) characteristic. For the level of optimal STB factors, the combination is A2 = 1, B1 = 0.06, and C1 = 0.175. The optimal combination can be obtained from the eighth combination in orthogonal arrays L9 (33) in Table 3, which indicates a higher rotational speed.

Bottom Line: Together with supporting experiments, simulation based on two-phase flow theory is used in this study, and the feasibility of this novel valve design is confirmed.From both the experimental results and the simulated results, it is evident that the narrowest channel width and the contact angle are the primary factors influencing valve burst frequency.These can be used as the main controlling factors during the design.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Power Mechanical Engineering, National Formosa University, Taiwan. samlin7@ms41.hinet.net.

ABSTRACT
CD-ELISA uses the microfluidic ranking method and centrifugal force to control the testing solution as it flows into the reaction region. The most challenging part of CD-ELISA is controlling the flow process for different biological testing solutions, i.e. the controlling sequence for the microfluidic channel valves. The microfluidic channel valve is therefore the most important fluid channel structure for CD-ELISA. In this study, we propose a valve design suitable for a wide range rotational speeds which can be applied for mass production (molding). Together with supporting experiments, simulation based on two-phase flow theory is used in this study, and the feasibility of this novel valve design is confirmed. Influencing design factors for the microfluidic channel valves in CD-ELISA are investigated, including various shapes of the arc, distance d, radius r, the location of the center of the circle, and the contact angle. From both the experimental results and the simulated results, it is evident that the narrowest channel width and the contact angle are the primary factors influencing valve burst frequency. These can be used as the main controlling factors during the design.

No MeSH data available.