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Potential stemness of frozen-thawed testicular biopsies without sperm in infertile men included into the in vitro fertilization programme.

Stimpfel M, Skutella T, Kubista M, Malicev E, Conrad S, Virant-Klun I - J. Biomed. Biotechnol. (2012)

Bottom Line: The patients were diagnosed with Sertoli Cell-Only Syndrome alone or combined with maturation arrest.High throughput real-time quantitative PCR followed by multivariate analysis revealed the formation of distinct cell clusters reflecting high degree of similarity and some of these cell clusters expressed the genes characteristic for pluripotent stem cells.In the presence of the follicular fluid, prepared as serum, putative testicular stem cells showed a certain degree of plasticity, and spontaneously differentiated into adipose-like and neuronal-like cells.

View Article: PubMed Central - PubMed

Affiliation: Reproductive Unit, Department of Obstetrics and Gynaecology, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia.

ABSTRACT
We describe the potential stemness of a small amount of frozen-thawed testicular tissue without sperm obtained by biopsy from six patients undergoing assisted reproductive treatment. The patients were diagnosed with Sertoli Cell-Only Syndrome alone or combined with maturation arrest. Trying to provide the natural stem cell niche for cultured stem cells, all isolated cells from enzymatically degraded biopsies where cultured together in different culture media and the presence of putative mesenchymal and putative pluripotent ES-like stem cells was indicated using different methods. High throughput real-time quantitative PCR followed by multivariate analysis revealed the formation of distinct cell clusters reflecting high degree of similarity and some of these cell clusters expressed the genes characteristic for pluripotent stem cells. In the presence of the follicular fluid, prepared as serum, putative testicular stem cells showed a certain degree of plasticity, and spontaneously differentiated into adipose-like and neuronal-like cells. Additionally, using differentiation protocols putative testicular stem cells were differentiated into neuronal- and pancreatic-like cells. This study shows that in assisted reproduction programmes, testicular tissue with no sperm might be an important source of stem cells, although it is discarded in daily medical practice; this requires further research.

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Cell culture grown in a DMEM/F12 medium supplemented with FBS. (a) The second passage of cell culture on day 76. (inverted microscope, Hoffman). Scale Bar: 100 μm.
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fig1: Cell culture grown in a DMEM/F12 medium supplemented with FBS. (a) The second passage of cell culture on day 76. (inverted microscope, Hoffman). Scale Bar: 100 μm.

Mentions: The cell cultures consisted of adherent fibroblasts or fibroblast-like cells. During passages, these cell cultures showed very little change. After passages up to 114 days, the cell cultures were morphologically similar to what they were at the beginning (Figure 1).


Potential stemness of frozen-thawed testicular biopsies without sperm in infertile men included into the in vitro fertilization programme.

Stimpfel M, Skutella T, Kubista M, Malicev E, Conrad S, Virant-Klun I - J. Biomed. Biotechnol. (2012)

Cell culture grown in a DMEM/F12 medium supplemented with FBS. (a) The second passage of cell culture on day 76. (inverted microscope, Hoffman). Scale Bar: 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303891&req=5

fig1: Cell culture grown in a DMEM/F12 medium supplemented with FBS. (a) The second passage of cell culture on day 76. (inverted microscope, Hoffman). Scale Bar: 100 μm.
Mentions: The cell cultures consisted of adherent fibroblasts or fibroblast-like cells. During passages, these cell cultures showed very little change. After passages up to 114 days, the cell cultures were morphologically similar to what they were at the beginning (Figure 1).

Bottom Line: The patients were diagnosed with Sertoli Cell-Only Syndrome alone or combined with maturation arrest.High throughput real-time quantitative PCR followed by multivariate analysis revealed the formation of distinct cell clusters reflecting high degree of similarity and some of these cell clusters expressed the genes characteristic for pluripotent stem cells.In the presence of the follicular fluid, prepared as serum, putative testicular stem cells showed a certain degree of plasticity, and spontaneously differentiated into adipose-like and neuronal-like cells.

View Article: PubMed Central - PubMed

Affiliation: Reproductive Unit, Department of Obstetrics and Gynaecology, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia.

ABSTRACT
We describe the potential stemness of a small amount of frozen-thawed testicular tissue without sperm obtained by biopsy from six patients undergoing assisted reproductive treatment. The patients were diagnosed with Sertoli Cell-Only Syndrome alone or combined with maturation arrest. Trying to provide the natural stem cell niche for cultured stem cells, all isolated cells from enzymatically degraded biopsies where cultured together in different culture media and the presence of putative mesenchymal and putative pluripotent ES-like stem cells was indicated using different methods. High throughput real-time quantitative PCR followed by multivariate analysis revealed the formation of distinct cell clusters reflecting high degree of similarity and some of these cell clusters expressed the genes characteristic for pluripotent stem cells. In the presence of the follicular fluid, prepared as serum, putative testicular stem cells showed a certain degree of plasticity, and spontaneously differentiated into adipose-like and neuronal-like cells. Additionally, using differentiation protocols putative testicular stem cells were differentiated into neuronal- and pancreatic-like cells. This study shows that in assisted reproduction programmes, testicular tissue with no sperm might be an important source of stem cells, although it is discarded in daily medical practice; this requires further research.

Show MeSH
Related in: MedlinePlus