Limits...
Characterization, tissue expression, and imprinting analysis of the porcine CDKN1C and NAP1L4 genes.

Li S, Li J, Tian J, Dong R, Wei J, Qiu X, Jiang C - J. Biomed. Biotechnol. (2012)

Bottom Line: By the IMpRH panel, porcine CDKN1C and NAP1L4 genes were assigned to porcine chromosome 2, closely linked with IMpRH06175 and with LOD of 15.78 and 17.94, respectively.The transcription levels of porcine CDKN1C and NAP1L4 were significantly higher in placenta than in other neonatal tissues (P < 0.01) although both genes showed the highest expression levels in the lung and kidney of one-month pigs (P < 0.01).Imprinting analysis demonstrated that in pigs, CDKN1C was maternally expressed in neonatal heart, tongue, bladder, ovary, spleen, liver, skeletal muscle, stomach, small intestine, and placenta and biallelically expressed in lung and kidney, while NAP1L4 was biallelically expressed in the 12 neonatal tissues examined.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science, Southwest University, Chongqing, China.

ABSTRACT
CDKN1C and NAP1L4 in human CDKN1C/KCNQ1OT1 imprinted domain are two key candidate genes responsible for BWS (Beckwith-Wiedemann syndrome) and cancer. In order to increase understanding of these genes in pigs, their cDNAs are characterized in this paper. By the IMpRH panel, porcine CDKN1C and NAP1L4 genes were assigned to porcine chromosome 2, closely linked with IMpRH06175 and with LOD of 15.78 and 17.94, respectively. By real-time quantitative RT-PCR and polymorphism-based method, tissue and allelic expression of both genes were determined using F1 pigs of Rongchang and Landrace reciprocal crosses. The transcription levels of porcine CDKN1C and NAP1L4 were significantly higher in placenta than in other neonatal tissues (P < 0.01) although both genes showed the highest expression levels in the lung and kidney of one-month pigs (P < 0.01). Imprinting analysis demonstrated that in pigs, CDKN1C was maternally expressed in neonatal heart, tongue, bladder, ovary, spleen, liver, skeletal muscle, stomach, small intestine, and placenta and biallelically expressed in lung and kidney, while NAP1L4 was biallelically expressed in the 12 neonatal tissues examined. It is concluded that imprinting of CDKN1C is conservative in mammals but has tissue specificity in pigs, and imprinting of NAP1L4 is controversial in mammalian species.

Show MeSH

Related in: MedlinePlus

Maternal or biallelic expression of CDKN1C (a) and NAP1L4 (b) in 0-day pigs. RC: Rongchang pigs, LD: Landrace pigs, P: placenta, L: lung, K: kidney, Sm: skeletal muscle. Arrows indicate SNP sites.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3303864&req=5

fig2: Maternal or biallelic expression of CDKN1C (a) and NAP1L4 (b) in 0-day pigs. RC: Rongchang pigs, LD: Landrace pigs, P: placenta, L: lung, K: kidney, Sm: skeletal muscle. Arrows indicate SNP sites.

Mentions: Alignment of Rongchang and Landrace sequences revealed an SNP (A/G) at position 916 of CDKN1C (HQ679903). Since the polymorphism did not provide any available RFLP site, direct sequencing of RT-PCR products was used for the discrimination of the expressed alleles. Sequencing of the PCR products revealed that three newborn pigs from RC × LD and four from LD × RC occurred to be heterozygous for the SNP, while their parental pigs were homozygous for the allele A and G, respectively. The (RC × LD) F1 pigs only expressed the Landrace allele G, and (LD × RC) F1 pigs only expressed the Rongchang A allele in placenta (Figure 2(a)) as well as in tongue, bladder, ovary, spleen, liver, skeletal muscle, stomach, and small intestine. However, biallelic expression of porcine CDKN1C was detected in lung and kidney (Figure 2(a)).


Characterization, tissue expression, and imprinting analysis of the porcine CDKN1C and NAP1L4 genes.

Li S, Li J, Tian J, Dong R, Wei J, Qiu X, Jiang C - J. Biomed. Biotechnol. (2012)

Maternal or biallelic expression of CDKN1C (a) and NAP1L4 (b) in 0-day pigs. RC: Rongchang pigs, LD: Landrace pigs, P: placenta, L: lung, K: kidney, Sm: skeletal muscle. Arrows indicate SNP sites.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303864&req=5

fig2: Maternal or biallelic expression of CDKN1C (a) and NAP1L4 (b) in 0-day pigs. RC: Rongchang pigs, LD: Landrace pigs, P: placenta, L: lung, K: kidney, Sm: skeletal muscle. Arrows indicate SNP sites.
Mentions: Alignment of Rongchang and Landrace sequences revealed an SNP (A/G) at position 916 of CDKN1C (HQ679903). Since the polymorphism did not provide any available RFLP site, direct sequencing of RT-PCR products was used for the discrimination of the expressed alleles. Sequencing of the PCR products revealed that three newborn pigs from RC × LD and four from LD × RC occurred to be heterozygous for the SNP, while their parental pigs were homozygous for the allele A and G, respectively. The (RC × LD) F1 pigs only expressed the Landrace allele G, and (LD × RC) F1 pigs only expressed the Rongchang A allele in placenta (Figure 2(a)) as well as in tongue, bladder, ovary, spleen, liver, skeletal muscle, stomach, and small intestine. However, biallelic expression of porcine CDKN1C was detected in lung and kidney (Figure 2(a)).

Bottom Line: By the IMpRH panel, porcine CDKN1C and NAP1L4 genes were assigned to porcine chromosome 2, closely linked with IMpRH06175 and with LOD of 15.78 and 17.94, respectively.The transcription levels of porcine CDKN1C and NAP1L4 were significantly higher in placenta than in other neonatal tissues (P < 0.01) although both genes showed the highest expression levels in the lung and kidney of one-month pigs (P < 0.01).Imprinting analysis demonstrated that in pigs, CDKN1C was maternally expressed in neonatal heart, tongue, bladder, ovary, spleen, liver, skeletal muscle, stomach, small intestine, and placenta and biallelically expressed in lung and kidney, while NAP1L4 was biallelically expressed in the 12 neonatal tissues examined.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science, Southwest University, Chongqing, China.

ABSTRACT
CDKN1C and NAP1L4 in human CDKN1C/KCNQ1OT1 imprinted domain are two key candidate genes responsible for BWS (Beckwith-Wiedemann syndrome) and cancer. In order to increase understanding of these genes in pigs, their cDNAs are characterized in this paper. By the IMpRH panel, porcine CDKN1C and NAP1L4 genes were assigned to porcine chromosome 2, closely linked with IMpRH06175 and with LOD of 15.78 and 17.94, respectively. By real-time quantitative RT-PCR and polymorphism-based method, tissue and allelic expression of both genes were determined using F1 pigs of Rongchang and Landrace reciprocal crosses. The transcription levels of porcine CDKN1C and NAP1L4 were significantly higher in placenta than in other neonatal tissues (P < 0.01) although both genes showed the highest expression levels in the lung and kidney of one-month pigs (P < 0.01). Imprinting analysis demonstrated that in pigs, CDKN1C was maternally expressed in neonatal heart, tongue, bladder, ovary, spleen, liver, skeletal muscle, stomach, small intestine, and placenta and biallelically expressed in lung and kidney, while NAP1L4 was biallelically expressed in the 12 neonatal tissues examined. It is concluded that imprinting of CDKN1C is conservative in mammals but has tissue specificity in pigs, and imprinting of NAP1L4 is controversial in mammalian species.

Show MeSH
Related in: MedlinePlus