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A water soluble CoQ10 formulation improves intracellular distribution and promotes mitochondrial respiration in cultured cells.

Bergamini C, Moruzzi N, Sblendido A, Lenaz G, Fato R - PLoS ONE (2012)

Bottom Line: Controversial clinical and in vitro results are mainly due to the high hydrophobicity of this compound, which reduces its bioavailability.Our results show that the water soluble formulation is more efficient in increasing ubiquinone levels.The improved cellular energy metabolism related to increased CoQ(10) content represents a strong rationale for the clinical use of coenzyme Q(10) and highlights the biological effects of Qter®, that make it the eligible CoQ(10) formulation for the ubiquinone supplementation.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry G. Moruzzi, University of Bologna, Bologna, Italy.

ABSTRACT

Background: Mitochondria are both the cellular powerhouse and the major source of reactive oxygen species. Coenzyme Q(10) plays a key role in mitochondrial energy production and is recognized as a powerful antioxidant. For these reasons it can be argued that higher mitochondrial ubiquinone levels may enhance the energy state and protect from oxidative stress. Despite the large number of clinical studies on the effect of CoQ(10) supplementation, there are very few experimental data about the mitochondrial ubiquinone content and the cellular bioenergetic state after supplementation. Controversial clinical and in vitro results are mainly due to the high hydrophobicity of this compound, which reduces its bioavailability.

Principal findings: We measured the cellular and mitochondrial ubiquinone content in two cell lines (T67 and H9c2) after supplementation with a hydrophilic CoQ(10) formulation (Qter®) and native CoQ(10). Our results show that the water soluble formulation is more efficient in increasing ubiquinone levels. We have evaluated the bioenergetics effect of ubiquinone treatment, demonstrating that intracellular CoQ(10) content after Qter supplementation positively correlates with an improved mitochondrial functionality (increased oxygen consumption rate, transmembrane potential, ATP synthesis) and resistance to oxidative stress.

Conclusions: The improved cellular energy metabolism related to increased CoQ(10) content represents a strong rationale for the clinical use of coenzyme Q(10) and highlights the biological effects of Qter®, that make it the eligible CoQ(10) formulation for the ubiquinone supplementation.

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Related in: MedlinePlus

Malondialdehyde (MDA) levels in T67 cells treated with native CoQ10 or Qter.Cells were pre-treated for 24 hours with native CoQ10 and QTer (100 nM and 10 µM). Panel A shows the MDA levels in the absence of external oxidative stress. Panel B shows the MDA levels after 30 minutes exposure to 100 µM TBH. Data are the mean of two different experimental determinations and are normalized on total protein content.
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pone-0033712-g006: Malondialdehyde (MDA) levels in T67 cells treated with native CoQ10 or Qter.Cells were pre-treated for 24 hours with native CoQ10 and QTer (100 nM and 10 µM). Panel A shows the MDA levels in the absence of external oxidative stress. Panel B shows the MDA levels after 30 minutes exposure to 100 µM TBH. Data are the mean of two different experimental determinations and are normalized on total protein content.

Mentions: Figure 5A and 5B report the MitoSOX Red staining of H9c2 cells without (Fig. 5A) and with (Fig. 5B) 100 nM Qter® pre-treatment for 24 hours: the lower staining observed in Figure 5B suggests that cellular CoQ10 supplementation reduces the ROS level also in absence of an oxidative insult. In Figure 5C is reported the MitoSOX Red fluorescence intensity obtained by Image J software analysis. ROS damage can be evaluated by measuring the presence of oxidative products such as malondialdehyde (MDA) and conjugated dienes. We observed that Qter treatment caused a statistically significant reduction of all lipid oxidation markers. Figure 6A and 6B show the MDA levels in T67 cells, both in absence (Fig. 6A) and in presence (Fig. 6B) of an oxidative insult, induced by treatment with 100 µM TBH. Even in this case it is possible to appreciate the higher efficiency of Qter® supplementation with respect to native CoQ10.


A water soluble CoQ10 formulation improves intracellular distribution and promotes mitochondrial respiration in cultured cells.

Bergamini C, Moruzzi N, Sblendido A, Lenaz G, Fato R - PLoS ONE (2012)

Malondialdehyde (MDA) levels in T67 cells treated with native CoQ10 or Qter.Cells were pre-treated for 24 hours with native CoQ10 and QTer (100 nM and 10 µM). Panel A shows the MDA levels in the absence of external oxidative stress. Panel B shows the MDA levels after 30 minutes exposure to 100 µM TBH. Data are the mean of two different experimental determinations and are normalized on total protein content.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303850&req=5

pone-0033712-g006: Malondialdehyde (MDA) levels in T67 cells treated with native CoQ10 or Qter.Cells were pre-treated for 24 hours with native CoQ10 and QTer (100 nM and 10 µM). Panel A shows the MDA levels in the absence of external oxidative stress. Panel B shows the MDA levels after 30 minutes exposure to 100 µM TBH. Data are the mean of two different experimental determinations and are normalized on total protein content.
Mentions: Figure 5A and 5B report the MitoSOX Red staining of H9c2 cells without (Fig. 5A) and with (Fig. 5B) 100 nM Qter® pre-treatment for 24 hours: the lower staining observed in Figure 5B suggests that cellular CoQ10 supplementation reduces the ROS level also in absence of an oxidative insult. In Figure 5C is reported the MitoSOX Red fluorescence intensity obtained by Image J software analysis. ROS damage can be evaluated by measuring the presence of oxidative products such as malondialdehyde (MDA) and conjugated dienes. We observed that Qter treatment caused a statistically significant reduction of all lipid oxidation markers. Figure 6A and 6B show the MDA levels in T67 cells, both in absence (Fig. 6A) and in presence (Fig. 6B) of an oxidative insult, induced by treatment with 100 µM TBH. Even in this case it is possible to appreciate the higher efficiency of Qter® supplementation with respect to native CoQ10.

Bottom Line: Controversial clinical and in vitro results are mainly due to the high hydrophobicity of this compound, which reduces its bioavailability.Our results show that the water soluble formulation is more efficient in increasing ubiquinone levels.The improved cellular energy metabolism related to increased CoQ(10) content represents a strong rationale for the clinical use of coenzyme Q(10) and highlights the biological effects of Qter®, that make it the eligible CoQ(10) formulation for the ubiquinone supplementation.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry G. Moruzzi, University of Bologna, Bologna, Italy.

ABSTRACT

Background: Mitochondria are both the cellular powerhouse and the major source of reactive oxygen species. Coenzyme Q(10) plays a key role in mitochondrial energy production and is recognized as a powerful antioxidant. For these reasons it can be argued that higher mitochondrial ubiquinone levels may enhance the energy state and protect from oxidative stress. Despite the large number of clinical studies on the effect of CoQ(10) supplementation, there are very few experimental data about the mitochondrial ubiquinone content and the cellular bioenergetic state after supplementation. Controversial clinical and in vitro results are mainly due to the high hydrophobicity of this compound, which reduces its bioavailability.

Principal findings: We measured the cellular and mitochondrial ubiquinone content in two cell lines (T67 and H9c2) after supplementation with a hydrophilic CoQ(10) formulation (Qter®) and native CoQ(10). Our results show that the water soluble formulation is more efficient in increasing ubiquinone levels. We have evaluated the bioenergetics effect of ubiquinone treatment, demonstrating that intracellular CoQ(10) content after Qter supplementation positively correlates with an improved mitochondrial functionality (increased oxygen consumption rate, transmembrane potential, ATP synthesis) and resistance to oxidative stress.

Conclusions: The improved cellular energy metabolism related to increased CoQ(10) content represents a strong rationale for the clinical use of coenzyme Q(10) and highlights the biological effects of Qter®, that make it the eligible CoQ(10) formulation for the ubiquinone supplementation.

Show MeSH
Related in: MedlinePlus