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Molecular epidemiology of Neisseria meningitidis serogroup B in Brazil.

de Filippis I, de Lemos AP, Hostetler JB, Wollenberg K, Sacchi CT, Dunning Hotopp JC, Harrison LH, Bash MC, Prevots DR - PLoS ONE (2012)

Bottom Line: OMP variation was associated with but not predicted by cc or ST.The majority of nadA were similar to reference allele 1.A predominant serogroup B lineage has circulated in Brazil for over a decade with significant regional and temporal diversity in ST, fetA, and porB, but not in nadA and fHbp.

View Article: PubMed Central - PubMed

Affiliation: National Quality Control Institute (INCQS), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil.

ABSTRACT

Background: Neisseria meningitidis serogroup B has been predominant in Brazil, but no broadly effective vaccine is available to prevent endemic meningococcal disease. To understand genetic diversity among serogroup B strains in Brazil, we selected a nationally representative sample of clinical disease isolates from 2004, and a temporally representative sample for the state of São Paulo (1988-2006) for study (n = 372).

Methods: We performed multi-locus sequence typing (MLST) and sequence analysis of five outer membrane protein (OMP) genes, including novel vaccine targets fHbp and nadA.

Results: In 2004, strain B:4:P1.15,19 clonal complex ST-32/ET-5 (cc32) predominated throughout Brazil; regional variation in MLST sequence type (ST), fetA, and porB was significant but diversity was limited for nadA and fHbp. Between 1988 and 1996, the São Paulo isolates shifted from clonal complex ST-41/44/Lineage 3 (cc41/44) to cc32. OMP variation was associated with but not predicted by cc or ST. Overall, fHbp variant 1/subfamily B was present in 80% of isolates and showed little diversity. The majority of nadA were similar to reference allele 1.

Conclusions: A predominant serogroup B lineage has circulated in Brazil for over a decade with significant regional and temporal diversity in ST, fetA, and porB, but not in nadA and fHbp.

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Related in: MedlinePlus

Maximum-likelihood phylogeny of the MLST DNA sequences from São Paulo.Annotations are for OMP the neisseria.org (http://pubmlst.org/neisseria.org/) (PorA, PorB, FetA, and FHbp, respectively), clonal complex, and year of collection. Numbers above the branches are bootstrap percentages.
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pone-0033016-g003: Maximum-likelihood phylogeny of the MLST DNA sequences from São Paulo.Annotations are for OMP the neisseria.org (http://pubmlst.org/neisseria.org/) (PorA, PorB, FetA, and FHbp, respectively), clonal complex, and year of collection. Numbers above the branches are bootstrap percentages.

Mentions: By phylogenetic analysis of concatenated MLST genes of the São Paulo samples, the year of collection did not correspond to the branching structure of the tree (Figure 3) and the dominant STs were found in all years. As with 2004 isolates, the São Paulo OMP diversity was low overall within the cc32 clade and the diversity observed did not correspond to phylogenetic structure, year of isolation or other OMP type.


Molecular epidemiology of Neisseria meningitidis serogroup B in Brazil.

de Filippis I, de Lemos AP, Hostetler JB, Wollenberg K, Sacchi CT, Dunning Hotopp JC, Harrison LH, Bash MC, Prevots DR - PLoS ONE (2012)

Maximum-likelihood phylogeny of the MLST DNA sequences from São Paulo.Annotations are for OMP the neisseria.org (http://pubmlst.org/neisseria.org/) (PorA, PorB, FetA, and FHbp, respectively), clonal complex, and year of collection. Numbers above the branches are bootstrap percentages.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303791&req=5

pone-0033016-g003: Maximum-likelihood phylogeny of the MLST DNA sequences from São Paulo.Annotations are for OMP the neisseria.org (http://pubmlst.org/neisseria.org/) (PorA, PorB, FetA, and FHbp, respectively), clonal complex, and year of collection. Numbers above the branches are bootstrap percentages.
Mentions: By phylogenetic analysis of concatenated MLST genes of the São Paulo samples, the year of collection did not correspond to the branching structure of the tree (Figure 3) and the dominant STs were found in all years. As with 2004 isolates, the São Paulo OMP diversity was low overall within the cc32 clade and the diversity observed did not correspond to phylogenetic structure, year of isolation or other OMP type.

Bottom Line: OMP variation was associated with but not predicted by cc or ST.The majority of nadA were similar to reference allele 1.A predominant serogroup B lineage has circulated in Brazil for over a decade with significant regional and temporal diversity in ST, fetA, and porB, but not in nadA and fHbp.

View Article: PubMed Central - PubMed

Affiliation: National Quality Control Institute (INCQS), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil.

ABSTRACT

Background: Neisseria meningitidis serogroup B has been predominant in Brazil, but no broadly effective vaccine is available to prevent endemic meningococcal disease. To understand genetic diversity among serogroup B strains in Brazil, we selected a nationally representative sample of clinical disease isolates from 2004, and a temporally representative sample for the state of São Paulo (1988-2006) for study (n = 372).

Methods: We performed multi-locus sequence typing (MLST) and sequence analysis of five outer membrane protein (OMP) genes, including novel vaccine targets fHbp and nadA.

Results: In 2004, strain B:4:P1.15,19 clonal complex ST-32/ET-5 (cc32) predominated throughout Brazil; regional variation in MLST sequence type (ST), fetA, and porB was significant but diversity was limited for nadA and fHbp. Between 1988 and 1996, the São Paulo isolates shifted from clonal complex ST-41/44/Lineage 3 (cc41/44) to cc32. OMP variation was associated with but not predicted by cc or ST. Overall, fHbp variant 1/subfamily B was present in 80% of isolates and showed little diversity. The majority of nadA were similar to reference allele 1.

Conclusions: A predominant serogroup B lineage has circulated in Brazil for over a decade with significant regional and temporal diversity in ST, fetA, and porB, but not in nadA and fHbp.

Show MeSH
Related in: MedlinePlus