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Biodegradation of pig manure by the housefly, Musca domestica: a viable ecological strategy for pig manure management.

Čičková H, Pastor B, Kozánek M, Martínez-Sánchez A, Rojo S, Takáč P - PLoS ONE (2012)

Bottom Line: Larval survival ranged from 46.9±2.1%, in manure without sawdust, to 76.8±11.9% in centrifuged slurry.Larval development took 6-11 days, depending on the manure type.Processing of 1 kg of wet manure produced 43.9-74.3 g of housefly pupae and the weight of the residue after biodegradation decreased to 0.18-0.65 kg, with marked differences among manure types.

View Article: PubMed Central - PubMed

Affiliation: Institute of Zoology, Slovak Academy of Sciences, Bratislava, Slovakia. helena.cickova@savba.sk

ABSTRACT
The technology for biodegradation of pig manure by using houseflies in a pilot plant capable of processing 500-700 kg of pig manure per week is described. A single adult cage loaded with 25,000 pupae produced 177.7±32.0 ml of eggs in a 15-day egg-collection period. With an inoculation ratio of 0.4-1.0 ml eggs/kg of manure, the amount of eggs produced by a single cage can suffice for the biodegradation of 178-444 kg of manure. Larval development varied among four different types of pig manure (centrifuged slurry, fresh manure, manure with sawdust, manure without sawdust). Larval survival ranged from 46.9±2.1%, in manure without sawdust, to 76.8±11.9% in centrifuged slurry. Larval development took 6-11 days, depending on the manure type. Processing of 1 kg of wet manure produced 43.9-74.3 g of housefly pupae and the weight of the residue after biodegradation decreased to 0.18-0.65 kg, with marked differences among manure types. Recommendations for the operation of industrial-scale biodegradation facilities are presented and discussed.

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Floor plans of the biodegradation facilities.(A) Miloslavov pilot plant, (B) Alpuente facility.
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pone-0032798-g003: Floor plans of the biodegradation facilities.(A) Miloslavov pilot plant, (B) Alpuente facility.

Mentions: The Miloslavov experimental pilot plant was located within the area of a pig farm. The key elements of the facility include the adult room, where the egg-production colony flies are kept, and the larval rearing room, where the manure biodegradation process takes place. In addition, the facility includes a washing/work room (for egg collection, handling of manure, and cleaning procedures), a drying room (for drying processed manure, from which the larvae/pupae were removed or killed), a storage room (for keeping the products of biodegradation), a laboratory (for running experiments), a toilet, and a lobby connecting these rooms (Fig. 3A). The facility and all equipment were cleaned on a regular basis with commercial sodium hypochlorite-based disinfectant. Equipment and surfaces coming into direct contact with manure and/or flies (i. e. rearing trays, oviposition cloths, screens, cages, shovels, etc.) were disinfected immediately after use. Prevention of fly escape was ensured by sticky tapes placed in both egg production and biodegradation rooms and by a UV trap in the biodegradation room.


Biodegradation of pig manure by the housefly, Musca domestica: a viable ecological strategy for pig manure management.

Čičková H, Pastor B, Kozánek M, Martínez-Sánchez A, Rojo S, Takáč P - PLoS ONE (2012)

Floor plans of the biodegradation facilities.(A) Miloslavov pilot plant, (B) Alpuente facility.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3303781&req=5

pone-0032798-g003: Floor plans of the biodegradation facilities.(A) Miloslavov pilot plant, (B) Alpuente facility.
Mentions: The Miloslavov experimental pilot plant was located within the area of a pig farm. The key elements of the facility include the adult room, where the egg-production colony flies are kept, and the larval rearing room, where the manure biodegradation process takes place. In addition, the facility includes a washing/work room (for egg collection, handling of manure, and cleaning procedures), a drying room (for drying processed manure, from which the larvae/pupae were removed or killed), a storage room (for keeping the products of biodegradation), a laboratory (for running experiments), a toilet, and a lobby connecting these rooms (Fig. 3A). The facility and all equipment were cleaned on a regular basis with commercial sodium hypochlorite-based disinfectant. Equipment and surfaces coming into direct contact with manure and/or flies (i. e. rearing trays, oviposition cloths, screens, cages, shovels, etc.) were disinfected immediately after use. Prevention of fly escape was ensured by sticky tapes placed in both egg production and biodegradation rooms and by a UV trap in the biodegradation room.

Bottom Line: Larval survival ranged from 46.9±2.1%, in manure without sawdust, to 76.8±11.9% in centrifuged slurry.Larval development took 6-11 days, depending on the manure type.Processing of 1 kg of wet manure produced 43.9-74.3 g of housefly pupae and the weight of the residue after biodegradation decreased to 0.18-0.65 kg, with marked differences among manure types.

View Article: PubMed Central - PubMed

Affiliation: Institute of Zoology, Slovak Academy of Sciences, Bratislava, Slovakia. helena.cickova@savba.sk

ABSTRACT
The technology for biodegradation of pig manure by using houseflies in a pilot plant capable of processing 500-700 kg of pig manure per week is described. A single adult cage loaded with 25,000 pupae produced 177.7±32.0 ml of eggs in a 15-day egg-collection period. With an inoculation ratio of 0.4-1.0 ml eggs/kg of manure, the amount of eggs produced by a single cage can suffice for the biodegradation of 178-444 kg of manure. Larval development varied among four different types of pig manure (centrifuged slurry, fresh manure, manure with sawdust, manure without sawdust). Larval survival ranged from 46.9±2.1%, in manure without sawdust, to 76.8±11.9% in centrifuged slurry. Larval development took 6-11 days, depending on the manure type. Processing of 1 kg of wet manure produced 43.9-74.3 g of housefly pupae and the weight of the residue after biodegradation decreased to 0.18-0.65 kg, with marked differences among manure types. Recommendations for the operation of industrial-scale biodegradation facilities are presented and discussed.

Show MeSH