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Oreocnide integrifolia Flavonoids Augment Reprogramming for Islet Neogenesis and β-Cell Regeneration in Pancreatectomized BALB/c Mice.

- Evid Based Complement Alternat Med (2012)

Bottom Line: Agents which can either trigger proliferation of β-cells or induce neogenesis of β-cells from precursors would be of pivotal role in reversing diabetic manifestations.Px animals displayed increased blood glucose levels and decreased insulin titres which were ameliorated by FRF supplementation.Additionally, transcripts levels of Ins1/2, Reg-3α/γ, Ngn-3, and Pdx-1 were upregulated during the initial 1 week.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, The M. S. University of Baroda, Gujarat Vadodara 390002, India.

ABSTRACT
Agents which can either trigger proliferation of β-cells or induce neogenesis of β-cells from precursors would be of pivotal role in reversing diabetic manifestations. We examined the role of flavonoid rich fraction (FRF) of Oreocnide integrifolia leaves using a mice model of experimental regeneration. BALB/c mice were subjected to ~70% pancreatectomy (Px) and supplemented with FRF for 7, 14, and 21 days after pancreatectomy. Px animals displayed increased blood glucose levels and decreased insulin titres which were ameliorated by FRF supplementation. FRF-treated mice demonstrated prominent newly formed islets budding off from ducts and depicting increased BrdU incorporation. Additionally, transcripts levels of Ins1/2, Reg-3α/γ, Ngn-3, and Pdx-1 were upregulated during the initial 1 week. The present study provides evidence of a nutraceutical contributing to islet neogenesis from ductal cells as the mode of β-cell regeneration and a potential therapeutic for clinical trials in management of diabetic manifestations.

No MeSH data available.


(a)–(d) Images represent BrdU incorporation in ducts, (e, f) acinar, and (g, h) glucagon cells after day 7 Px. White arrows denote BrdU-incorporated cells. Scale bar represents 50 μm. All values are expressed as mean + SEM of six animals (one-way ANOVA), where, •P < 0.05, ▪P < 0.01, ♦P < 0.001: sham was compared to other experimental groups; βP < 0.01, δP < 0.001: Px day 7 was compared to Px day 7 + FRF.
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fig4: (a)–(d) Images represent BrdU incorporation in ducts, (e, f) acinar, and (g, h) glucagon cells after day 7 Px. White arrows denote BrdU-incorporated cells. Scale bar represents 50 μm. All values are expressed as mean + SEM of six animals (one-way ANOVA), where, •P < 0.05, ▪P < 0.01, ♦P < 0.001: sham was compared to other experimental groups; βP < 0.01, δP < 0.001: Px day 7 was compared to Px day 7 + FRF.

Mentions: Insulin immunoreactivity along with BrdU incorporation in ducts (Figure 4(a)) by day 7 after Px showed clearly increased BrdU labelling. A quantitative estimate of BrdU label in the ducts revealed significantly higher labelling in Px + FRF than in Px pancreas (Figures 4(a) and 4(b)). In comparison, smaller ducts seemed to show relatively higher labelling than the larger ducts (Figure 4(c)). Moreover, Px + FRF-treated mice demonstrated CK-19/insulin coexpression within ducts (Figure 4(d)).


Oreocnide integrifolia Flavonoids Augment Reprogramming for Islet Neogenesis and β-Cell Regeneration in Pancreatectomized BALB/c Mice.

- Evid Based Complement Alternat Med (2012)

(a)–(d) Images represent BrdU incorporation in ducts, (e, f) acinar, and (g, h) glucagon cells after day 7 Px. White arrows denote BrdU-incorporated cells. Scale bar represents 50 μm. All values are expressed as mean + SEM of six animals (one-way ANOVA), where, •P < 0.05, ▪P < 0.01, ♦P < 0.001: sham was compared to other experimental groups; βP < 0.01, δP < 0.001: Px day 7 was compared to Px day 7 + FRF.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3303763&req=5

fig4: (a)–(d) Images represent BrdU incorporation in ducts, (e, f) acinar, and (g, h) glucagon cells after day 7 Px. White arrows denote BrdU-incorporated cells. Scale bar represents 50 μm. All values are expressed as mean + SEM of six animals (one-way ANOVA), where, •P < 0.05, ▪P < 0.01, ♦P < 0.001: sham was compared to other experimental groups; βP < 0.01, δP < 0.001: Px day 7 was compared to Px day 7 + FRF.
Mentions: Insulin immunoreactivity along with BrdU incorporation in ducts (Figure 4(a)) by day 7 after Px showed clearly increased BrdU labelling. A quantitative estimate of BrdU label in the ducts revealed significantly higher labelling in Px + FRF than in Px pancreas (Figures 4(a) and 4(b)). In comparison, smaller ducts seemed to show relatively higher labelling than the larger ducts (Figure 4(c)). Moreover, Px + FRF-treated mice demonstrated CK-19/insulin coexpression within ducts (Figure 4(d)).

Bottom Line: Agents which can either trigger proliferation of β-cells or induce neogenesis of β-cells from precursors would be of pivotal role in reversing diabetic manifestations.Px animals displayed increased blood glucose levels and decreased insulin titres which were ameliorated by FRF supplementation.Additionally, transcripts levels of Ins1/2, Reg-3α/γ, Ngn-3, and Pdx-1 were upregulated during the initial 1 week.

View Article: PubMed Central - PubMed

Affiliation: Department of Zoology, Faculty of Science, The M. S. University of Baroda, Gujarat Vadodara 390002, India.

ABSTRACT
Agents which can either trigger proliferation of β-cells or induce neogenesis of β-cells from precursors would be of pivotal role in reversing diabetic manifestations. We examined the role of flavonoid rich fraction (FRF) of Oreocnide integrifolia leaves using a mice model of experimental regeneration. BALB/c mice were subjected to ~70% pancreatectomy (Px) and supplemented with FRF for 7, 14, and 21 days after pancreatectomy. Px animals displayed increased blood glucose levels and decreased insulin titres which were ameliorated by FRF supplementation. FRF-treated mice demonstrated prominent newly formed islets budding off from ducts and depicting increased BrdU incorporation. Additionally, transcripts levels of Ins1/2, Reg-3α/γ, Ngn-3, and Pdx-1 were upregulated during the initial 1 week. The present study provides evidence of a nutraceutical contributing to islet neogenesis from ductal cells as the mode of β-cell regeneration and a potential therapeutic for clinical trials in management of diabetic manifestations.

No MeSH data available.